HPLC micro-fractionation coupled to a cell-based assay for automated on-line primary screening of calcium antagonistic components in plant extracts

xe4;ivi#Tammela, P&; Wennberg, Tero; Vuorela, Heikki; Vuorela, Pia
October 2004
Analytical & Bioanalytical Chemistry;Oct2004, Vol. 380 Issue 4, p614
Academic Journal
High performance liquid chromatography (HPLC) micro-fractionation was successfully coupled to an automated45Ca2+ uptake assay using GH4C1 cells for the separation of natural product extracts and for the primary detection of their calcium antagonistic components. The reliability of the procedure was first established with a reference solution consisting of pure compounds with a known effect on the Ca2+ uptake. No loss of activity was observed to occur after HPLC micro-fractionation. Extracts ofPeucedanum palustreandPinus sylvestris, showing high and no inhibition of Ca2+ uptake as total extracts, respectively, were analysed and the inhibitory activity of theP. palustreextract could be traced to two components, identified as columbianadin and isoimperatorin. As expected, no significant inhibition was observed with the micro-fractionatedP. sylvestrissamples. In summary, the procedure was found to be applicable for primary detection of calcium antagonistic components in complex matrices and to significantly reduce the time previously needed for bioactivity-guided isolation.


Related Articles

  • High Resolution/Fast RP-HPLC Analysis of Synthetic Peptides Using 3μm Polymeric Particles. Lloyd, Linda L.; Millichip, Mark I.; Mapp, Keeley J. // LC-GC Europe;Sep2003 Applications Book, p18 

    Studies the use of reversed-phase high performance liquid chromatography in the analysis of synthetic peptides. Use of a binary Knauer high-pressure mixing gradient system in performing the method; Illustration of the separation of four peptide standards; Effectiveness of the method for peptide...

  • Ofloxacin Photodegradation Products Possess Antimicrobial Activity. Sunderland, J.; Tobin, C.M.; White, L.O.; MacGowan, A.P.; Hedges, A.J. // Drugs;Dec1999 Supplement 2, Vol. 58 Issue 6, p171 

    Reports on the use of parallel-line bioassay and high performance liquid chromatography to compare estimated relative potencies. Materials and methods; Results; Discussion.

  • Adjusting Conditions for a Routine Reversed-Phase HPLC Assay, Part 1: Changing the Column. Snyder, Lloyd R.; Dolan, John W. // LC-GC Europe;Apr2005, Vol. 18 Issue 4, p196 

    High performance liquid chromatography methods used for routine analysis in a regulated environment can cost many thousands of dollars when one considers the cost to develop, validate and document the method. Once that investment is made, users are very reluctant to make changes. For this...

  • Method Translation in Liquid Chromatography. Majors, Ronald E. // LC-GC Europe;Aug2011, Vol. 24 Issue 8, p412 

    With the recent emphasis on high-throughput high performance liquid chromatography (HPLC) and ultrahigh-pressure liquid chromatography (UHPLC), chromatographers are looking for ways to improve productivity. This instalment discusses the use of method translation calculators to make conversions...

  • Column Pressure Considerations in Analytical HPLC. Majors, Ronald E. // LC-GC North America;Nov2007, Vol. 25 Issue 11, p1074 

    The article examines the role of pressure in high performance liquid chromatography (HPLC). It looks at the impact of ultrahigh pressures encountered in ultrahigh-pressure liquid (UHPLC) on chromatographic parameters. It also addresses the concern of pressure changes in column pressure dictated...

  • Method Translation in Liquid Chromatography. Majors, Ronald E. // LC-GC North America;Jun2011, Vol. 29 Issue 6, p476 

    The article focuses on the use of method translation in liquid chromatography. It discusses the high performance liquid chromatography (HPLC) and compares it with the gas chromatography (GC) method translation. It talks about the need to set performance goals to determine if an isocratic method...

  • Fluorinated HPLC Phases -- Looking Beyond C18 for Reversed-Phase HPLC. Przybyciel, Matthew // LC-GC Europe;Jan2006, Vol. 19 Issue 1, p19 

    The article presents information about alkyl- and phenyl-fluorinated phases in high performance liquid chromatography. Reversed-phase high performance liquid chromatography is one of the most extensively used forms of chromatography. On the other hand analysts widely use the C8 and C18...

  • Top 10 HPLC Column Myths. Majors, Ronald E. // LC-GC Europe;Jan2007, Vol. 20 Issue 1, p13 

    In any field there are often ‘misconceptions’ or ‘myths’ that are perpetuated and passed on to the next generation. These myths are often driven by a lack of understanding by practitioners of the real issues. In this instalment of ‘Column Watch’, the 10...

  • Fluorinated HPLC Phases -- Looking Beyond C18 for Reversed-Phase HPLC. Przybyciel, Matthew // LC-GC North America;Jun2005, Vol. 23 Issue 6, p554 

    Guest columnist Matt Przybyciel reviews the structure, behavior, and applications of both alkyland phenyl-fluorinated phases in HPLC. The author presents some guidelines on potential areas of application for alkyl and phenylfluorinated phases, in which conventional C8, C18, and phenyl phases...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics