TITLE

Simultaneous determination ofL-arginine and its mono- and dimethylated metabolites in human plasma by high-performance liquid chromatography-mass spectrometry

AUTHOR(S)
Huang, Lan-Fang; Guo, Fang-Qiu; Liang, Yi-Zeng; Li, Bo-Yang; Cheng, Ben-Mei
PUB. DATE
October 2004
SOURCE
Analytical & Bioanalytical Chemistry;Oct2004, Vol. 380 Issue 4, p643
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A simple, fast, sensitive, and reproducible isocratic liquid chromatography-mass spectrometry (LC-MS) method coupled with an atmospheric pressure chemical ionization (APCI) interface for simultaneous separation and determination ofL-arginine (ARG) and its methylated metabolites,N-monomethyl-L-arginine (MMA),N G,N G-dimethylarginine (asymmetric dimethyl arginine, ADMA), andN G,N' G-dimethylarginine (symmetric dimethyl arginine, SDMA), in human plasma is presented. Sample pretreatment is not required other than deproteinization with 5-sulfosalicylic acid (5-SSA). Satisfactory chromatographic separation was achieved on a 2.0×150-mm Shimadzu VP-ODS column by using a mobile phase consisting of water/acetonitrile (90/10, v/v) containing 0.5% trifluoroacetic acid (TFA). Positive selective ion monitoring (SIM) mode was chosen for quantification of each analyte. The positively protonated molecular ions [M+H]+ of ARG, MMA, ADMA, and SDMA were monitored atm/z175, 189, 203, and 203, respectively.L-Homoarginine was used as the internal standard (IS) for the assay. The limits of quantification (LOQs) were found to be 1.0 µmol L-1 for ARG, and 0.2 µmol L-1 for MMA, ADMA, and SDMA. The inter-assay precision and accuracy were in the range of 1.8-4.9% and -3.0-5.0%, respectively. The intra-assay precision and accuracy were in the order of 1.7-4.6 and -2.6-4.0%, respectively. The recoveries were between 90.0 and 106.6%. The levels of ARG, MMA, ADMA, and SDMA in human plasma were also determined using the developed method.
ACCESSION #
14854124

 

Related Articles

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics