TITLE

Helicobacter pyloriIncreases the Epithelial Permeability to a Food Antigen in Human Gastric Biopsies

AUTHOR(S)
Matysiak-Budnik, Tamara; Coffin, Benoît; Lavergne-Slove, Anne; Sabate, Jean-Marc; Mégraud, Francis; Heyman, Martine
PUB. DATE
February 2004
SOURCE
American Journal of Gastroenterology;Feb2004, Vol. 99 Issue 2, p225
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
AIM: We have previously shown thatH. pyloriincreases the passage of intact protein across the digestive epithelium,in vitroandin vivoin mice. The aim of this study was to test whetherH. pylorican alter gastric permeability to a food-type antigen in humans.METHODS: Antral and corpus gastric biopsies obtained fromH. pylori-positive andH. pylori-negative patients suffering from nonulcer dyspepsia with normal esogastroduodenal endoscopy were mounted in adapted Ussing chambers to measure gastric permeability. Electrical resistance (R, an index of epithelial integrity) and fluxes of14C-mannitol (JMan, a marker of paracellular permeability) and3H-horseradish peroxidase (HRP, a test protein of antigen transport and processing) under intact (JHRPi) and degraded (JD) form, across the biopsies, were measured.RESULTS: At the corpus level, biopsies obtained fromH. pylori-positive patients, as compared toH. pylori-negative patients, presented significantly higher intact—but not degraded—HRP fluxes (JHRPi= 446± 297 and 219± 265 ng/h cm2,and 3,575± 2,594 ng/h · cm2, respectively), and an increase in paracellular permeability (Jman= 0.35± 0.1vs0.24± 0.08μmol/h·cm2,, respectively). At the antrum level, a tendency to increased intact HRP fluxes inH. pylori-positive patients, as compared toH. pylori-negative patients, was observed (JHRPi = 284± 250vs207± 203 ng/h · cm2,), without modifications of paracellular permeability.CONCLUSIONS: H. pyloriinfection increases absorption of an intact food antigen across the corpus gastric mucosa. This phenomenon may contribute to the maintenance of gastric inflammation and could play a role in the development of allergic sensitization to dietary antigens in susceptible individuals.
ACCESSION #
14816717

 

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