Elevated Concentrations ofα-Defensins in Gastric Juice of Patients withHelicobacter pyloriInfection

Isomoto, Hajime; Mukae, Hiroshi; Ishimoto, Hiroshi; Date, Yukari; Nishi, Yoshito; Inoue, Kenichiro; Wada, Akihiro; Hirayama, Toshiya; Nakazato, Masamitsu; Kohno, Shigeru
October 2004
American Journal of Gastroenterology;Oct2004, Vol. 99 Issue 10, p1916
Academic Journal
OBJECTIVE: Defensins (α- andβ-defensins) are endogenous antimicrobial peptides. Little is known aboutα-defensins duringHelicobacter pyloriinfection.METHODS: The concentrations of human neutrophil peptides (HNP-1, -2, and -3), the major components of neutrophils-derivedα-defensins, were measured by radioimmunoassay (RIA) in plasma and gastric juice of 61H. pylori-infected and 33 uninfected subjects, and before and after anti-H. pyloritreatment in 12 patients withH. pylori-associated gastritis. Interleukin (IL)-8 concentrations in gastric juice were measured by enzyme-linked immunosorbent assay. Histological grades of gastritis and neutrophil counts (/mm2) infiltrating in the gastric mucosa were determined using two biopsy specimens taken from the antrum and corpus. Immunohistochemistry and reverse-phase high performance liquid chromatography (RP-HPLC) were used to identify HNPs 1–3.RESULTS: HNP 1–3 concentrations in gastric juice were significantly higher inH. pylori-positive than inH. pylori-negative patients and significantly decreased after cure. HNP 1–3 concentrations in gastric juice correlated with IL-8 levels and neutrophil densities in the gastric mucosa and were associated with histological degree of gastritis, especially the grades of activity. Intense immunoreactivity for anti-HNPs 1–3 antiserum was noted in infiltrating neutrophils inH. pylori-infected mucosa. In RP-HPLC analysis, all of the HNP 1–3 molecules were identified as their mature forms. Plasma HNP 1–3 concentrations were similar inH. pylori-infected and non-infected groups and showed no correlations with other parameters.CONCLUSIONS: We demonstrated significantly elevated levels of HNPs 1–3 in gastric juice duringH. pyloriinfection. The elevation of HNPs is presumably secondary toH.pylori-associated gastric inflammation involving neutrophil infiltration.


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