TITLE

Cdx1 induced intestinal metaplasia in the transgenic mouse stomach: comparative study with Cdx2 transgenic mice

AUTHOR(S)
Mutoh, H.; Sakurai, S.; Satoh, K.; Osawa, H.; Hakamata, Y.; Takeuchi, T.; Sugano, K.
PUB. DATE
October 2004
SOURCE
Gut;Oct2004, Vol. 53 Issue 10, p1416
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background and aims: Gastric intestinal metaplasia, which is mainly induced by Helicobacter pylori infection, is thought to be a precancerous lesion of gastric adenocarcinoma. Intestinal metaplastic mucosa expresses intestine specific homeobox genes, Cdx1 and Cdx2, in the human gastric mucosa. We and others have reported that ectopic expression of Cdx2 in the gastric epithelium generates intestinal metaplasia in the transgenic mouse model. Methods: To clarify the differences in the roles of Cdx1 and Cdx2 in intestinal metaplasia, we generated transgenic mice expressing Cdx1 in the gastric mucosa and compared Cdx1 induced gastric mucosal morphological changes with Cdx2 induced intestinal metaplasia. Results:The gastric mucosa in Cdx1 transgenic mice was completely replaced by intestinal metaplastic mucosa, consisting of all four intestinal epithelial cell types: absorptive enterocytes, goblet, enteroendocrine, and Paneth cells. Paneth cells, which were not recognised in Cdx2 transgenic mice, were in the upper portion of the intestinal metaplastic mucosa. Pseudopyloric gland metaplasia, which was induced in Cdx2 transgenic mice, was not recognised in Cdx1 transgenic mice. Proliferating cell nuclear antigen (PCNA) positive cells were diffusely scattered in Cdx1 induced intestinal metaplastic mucosa while PCNA positive cells in Cdx2 induced intestinal metaplastic mucosa were in the base of the metaplastic mucosa. Intestinal metaplastic mucosa of Cdx1 transgenic mouse stomach was significantly thicker than that of wild-type or Cdx2 transgenic mouse stomach. Conclusions:We have confirmed that Cdx1 induced gastric intestinal metaplasia but that it differed from Cdx2 induced intestinal metaplasia in differentiation, structure, and proliferation.
ACCESSION #
14674382

 

Related Articles

  • A better method for confirming Helicobacter pylori infection in Mongolian gerbils. Chao-Hung Kuo; Huang-Ming Hu; Pei-Yun Tsai; Sheau-Fang Yang; Lin-Li Chang; Jaw-Yuan Wang; Chen, Angela; Chang-Ming Jan; Wen-Ming Wang; Deng-Chyang Wu // Journal of Gastroenterology;2008, Vol. 43 Issue 1, p32 

    Background. Our aim was to evaluate the accuracy of the stool antigen test and the optimal time point for detecting Helicobacter pylori infection in a Mongolian gerbil model. Methods. We inoculated 8-week-old Mongolian gerbils with H. pylori (Vac A (+)/CagA(+)). The gerbil-infected model was...

  • The Association of H. pylori infection and patterns of erythematous gastric mucosa. Urita, Yoshihisa; Watanabe, Toshiyasu; Takemoto, Ikutaka; Tanaka, Hideki; Kawagoe, Naoyuki; Takeuchi, Motoi; Hike, Kazuo; Honda, Yoshiko; Nakajima, Hitoshi; Shimada, Nagato; Sugimoto, Motonobu // Gastroenterology Insights;2011, Vol. 3 Issue 1, p3 

    It has been uncertain what types of erythematous gastric mucosa are produced by Helicobacter pylori (H. pylori)-infection. We therefore design the present study to identify the type of erythematous mucosa associated with H. pylori-infection. A total of 590 consecutive Japanese patients (mean age...

  • Levels of Interleukin-18 Are Markedly Increased in Helicobacter pylori-Infected Gastric Mucosa among Patients with Specific IL18 Genotypes. Sakai, Kyoko; Kita, Masakazu; Sawai, Naoki; Shiomi, Satoshi; Yoshio Sumida; Kanemasa, Kazuyuki; Mitsufuji, Shoji; Imanishi, Jiro; Yamaoka, Yoshio // Journal of Infectious Diseases;6/15/2008, Vol. 197 Issue 12, p1752 

    Background. The cellular immune response in gastric mucosa infected with Helicobacter pylori is proposed to be predominantly of the T helper cell type 1 type. Methods. Interleukin (IL)-18, IL-12, and interferon (IFN)-γ levels were measured in gastric mucosal biopsy specimens by...

  • H pylori may predispose patients to gastric malignancies.  // Modern Medicine;Aug96, Vol. 64 Issue 8, p41 

    Presents an abstract of a research titled `Helicobacter pylori in Promotion of Gastric Carcinogenesis,' by M. Rugge, M. Cassaro, G. Leandro et al, published in the May 1996 issue of `Digestive Diseases Science.'

  • The villain in most peptic ulcers: Helicobacter pylori.  // Nutrition Health Review: The Consumer's Medical Journal;1994, Issue 70, p16 

    Reports on the treatment of `Helicobacter pylori' infections in patients with peptic ulcers. Combination drug therapy with antimicrobial drugs; H-2 blocker supplementation; Testing for presence of `H. pylori'; Relationship of `H. pylori' with gastric cancer.

  • Smoking is associated with increased risk for duodenal ulcer in Helicobacter pylori-infected patients in Taiwan. Chen, T.S.; Lee, Y.C.; Chang, F.Y.; Lee, S.D. // Gut;Sep2002 Supplement 2, Vol. 51, pA63 

    Background and Aims: Although Helicobacter pylori has been established as a major etiologic factor of chronic gastritis and makes an important contribution to peptic ulceration, the reason why H. pylori causes different gastroduodenal disease in different people is at present not clean The aim...

  • Correlation Between Gastrointestinal Symptoms and Gastric Leptin and Ghrelin Expression in Patients with Gastritis. Dae Jun; Oh Lee; Young Lee; Ho Choi; Tae Kim; Byung Yoon // Digestive Diseases & Sciences;Oct2007, Vol. 52 Issue 10, p2866 

    Abstract  The purpose of this study was to examine the changes in gastric ghrelin and leptin with respect to Helicobacter pylori infection and whether such changes affect the plasma levels of leptin and ghrelin. In addition, we examined the relationship between changes in gastric...

  • Development, Calibration, and Validation of a U.S. White Male Population-Based Simulation Model of Esophageal Adenocarcinoma. Chin Hur; Hayeck, Tristan J.; Yeh, Jennifer M.; Richards, Ethan B.; Spechler, Stuart J.; Gazelle, G. Scott; Chung Yin Kong // PLoS ONE;2010, Vol. 5 Issue 3, p1 

    Background: The incidence of esophageal adenocarcinoma (EAC) has risen rapidly in the U.S. and western world. The aim of the study was to begin the investigation of this rapid rise by developing, calibrating, and validating a mathematical disease simulation model of EAC using available...

  • Irritation of the human eye mucous membrane caused by airborne pollutants. Podlekareva, Daria; Pan, Zhiwei; Kjærgaard, Søren; Mølhave, Lars // International Archives of Occupational & Environmental Health;Jun2002, Vol. 75 Issue 5, p359 

    Objectives: The purpose of this study was to investigate the different irritative effects of carbon dioxide and n-butanol exposure on the ocular mucous membrane. Material and methods: Provocation by the gases was at the same sensory level, which was 50% of maximum on a linear scale. The...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics