Separation of Polar and Hydrophilic Compounds Using a Zwitterionic Stationary Phase in Hydrophilic Interaction Liquid Chromatography

Jonsson, Tobias; Appelbiad, Patrik
September 2004
LC-GC Europe;Sep2004 Supplement, Vol. 17, p57
Trade Publication
The article presents information about hydrophilic interaction liquid chromatography (HILIC) as a means of separating polar and hydrophilic compounds. This technique is orthogonal to reverse-phase chromatography. The separation mode in HILIC uses an eluent containing a high content of water miscible organic solvent to promote hydrophilic interactions between the analyte and water wetted hydrophilic stationary phase. ZIC ® -HILIC Phase is one of the best HLIC Phases commercially available.


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