Decreased Helicobacter pylon associated gastric carcinogenesis in mice lacking inducible nitric oxide synthase

Nam, K. T.; Oh, S. -Y.; Ahn, B.; Kim, Y. B.; Jang, D. D.; Yang, K. -H.; Hahm, K. -B.; Kim, D. -Y.
September 2004
Gut;Sep2004, Vol. 53 Issue 9, p1250
Academic Journal
Background and aims: Overproduction of nitric oxide via inducible nitric oxide synthase (iNOS) is suggested to be a significant pathogenic factor in Helicobacter Pylori induced gastritis. The purpose of this study was to examine the role of iNOS in H Pylori associated gastric carcinogenesis. Methods: Two types of mice were used in this study: iNOS deficient mice (iNOS-/-) and wild-type liltermates. Gastric cancer was generated in mice using a combination treatment comprising N-methyl-N-nitrosourea administration and H Pylori infection. Fifty weeks alter treatment, tumours in gastric tissues from both types of mice were examined using histopathology, immunohistochemistry, and immunoblotting for iNOS and 3-nitrolyrosine. Results: The overall incidence of gastric cancer at week 50 was significantly lower in iNOS-/- compared with iNOS wild-type mice (p


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