Challenge model cor Helicobacter pylon infection in human volunteers

Graham, D. Y.; Opekun, A. R.; Osato, M. S.; El-Zimaity, H. M. T.; Lee, C. K.; Yamooka, Y.; Qureshi, W. A.; Cadoz, M.; Monath, T. P.
September 2004
Gut;Sep2004, Vol. 53 Issue 9, p1235
Academic Journal
Background: A reliable challenge model is needed to evaluate Helicobacter Pylori vaccine candidates. Methods: A cog pathogen icily island negative, OipA positive, multiple antibiotic susceptible strain of H pylori obtained from an individual with mild gastritis (Baylor strain 100) was used to challenge volunteers. Volunteers received 40 mg of Famotidine at bedtime and 104-1010 cfu of H pylori in beef broth the next morning. Infection was confirmed by 13C urea breath test (13C-UBT), culture, and histology. Eradication therapy was given four or 12 weeks post challenge and eradication was confirmed by at least two separate UBTs, as well as culture and histology. Results: Twenty subjects (nine women and 11 men; aged 23-33 years) received a H Pylori challenge. Eighteen (90%) became infected. Mild to moderate dyspeptic symptoms occurred, peaked between days 9 and 12, and resolved. Vomitus from one subject contained >103 viable/mi H Pylori. By two weeks post challenge gastric histology showed typical chronic H Pylori gastritis with intense acute and chronic inflammation. The density of H Pylori (as assessed by cfu/biopsy) was similarly independent of the challenge dose. A minimal infectious dose was not found. Gastric mucosal interleukin 8 levels increased more than 20-Told by two weeks after the challenge. Conclusion: Challenge reliably resulted in H Pylori infection. Infection was associated with typical H Pylori gastritis with intense polymorphonuclear cell infiltration and interleukin 8 induction in gastric mucosa, despite absence of the cog pathogenicity island. Experimental H Pylori infection is one of the viable approaches to evaluate vaccine candidates.


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