TITLE

The fluorescence enhancement effect of the Tb-Gd-guanosine-5'-triphosphate-phen system and its analytical application

AUTHOR(S)
Zongshan Zhao; Jinghe Yang; Xia Wu; Benyu Su; Changxia Sun; Shulang Liu
PUB. DATE
September 2004
SOURCE
Analytical & Bioanalytical Chemistry;Sep2004, Vol. 380 Issue 1, p104
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
It has been found that Tb3+ can react with guanosine-5′-triphosphate (GTP) and o-phenanthroline (phen), resulting in the intrinsic fluorescence of Tb3+ This fluorescence can be enhanced by adding La3+, Gd3+, Lu3+. Sc3+, Y 3+. and so on, among which Gd3+ produces the greatest enhancement. These are new columinescence systems. The experiments indicate under optimum conditions, the fluorescence intensity of the Tb-Gd-GTP-phen system is proportional to the concentration of GTP over the range 1×10-9 to 3×10-5 mol/l. The detection limit is 3.1×10-10 mol/l. The proposed method provides the most sensitive fluorimetry of GTP so far. The mechanism of the Tb-Gd-GTP-phen system has also been studied. The data indicates that there is a large congeries of Tb-Gd-GTP-phen, and the fluorescence enhancement of the Tb-Gd-GTP-phen system is considered to originate from intramolecular and intermocular energy transfers, and the energy-insulating sheath of the Gd complex.
ACCESSION #
14392590

 

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