Speciation of selenium in selenium-enriched shiitake mushroom,Lentinula edodes

Ogra, Yasumitsu; Ishiwata, Kazuya; Encinar, Jorge Ruiz; Lobiñski, Ryszard; Suzuki, Kazuo T.
July 2004
Analytical & Bioanalytical Chemistry;Jul2004, Vol. 379 Issue 5/6, p861
Academic Journal
The major selenium compound in an aqueous extract of the most popular mushroom in Eastern Asian countries, shiitake (Lentinula edodes), fortified with selenium (Se) was identified by means of hyphenated techniques, i.e. HPLC-inductively coupled argon plasma mass spectrometry and HPLC-electrospray ionization mass spectrometry (HPLC-ICP MS and HPLC-ESI MS). Sixty-eight per cent of the total Se in the selenized shiitake was extracted with water, and 49.8% of the Se in the water extract was eluted in the high molecular mass fraction (>40,000 kDa) before incubation at 37 °C. After incubation, 40.6% of the Se in the water extract was eluted in a lower molecular mass fraction and the Se eluted in the high molecular mass fraction had decreased to 14.0%, suggesting that the major selenium compound in the water extract was initially in a form bound to macromolecule(s) and was then enzymatically liberated from the macromolecule(s). The retention time of the liberated selenium compound in HPLC-ICP MS matched that of selenomethionine (SeMet), and the masses of molecular and fragment ions detected by HPLC-ESI MS also suggested that the selenium compound was SeMet. The selenized shiitake accumulated Se as SeMet, and SeMet might be bound to the water extractable high molecular mass protein(s).


Related Articles

  • HPLC-ICP-MS and HPLC-ES-MS/MS characterization of synthetic seleno-arsenic compounds. Kanaki, Katerina; Pergantis, Spiros A. // Analytical & Bioanalytical Chemistry;Apr2007, Vol. 387 Issue 8, p2617 

    Various toxicological and metabolic interactions have been reported to exist between arsenic and selenium. In the present study, synthetic seleno-arsenic compounds, potentially suitable for probing metabolic interactions between these two elements, were prepared and tentatively characterized by...

  • Selenium speciation analysis of Misgurnus anguillicaudatus selenoprotein by HPLC-ICP-MS and HPLC-ESI-MS/MS. Gong, Like; Xu, Qingbing; Lee, Charles; Zhang, Hong // European Food Research & Technology;Jul2012, Vol. 235 Issue 1, p169 

    Analytical methods for selenium (Se) speciation were developed using high-performance liquid chromatography (HPLC) coupled to either inductively coupled plasma mass spectrometry (ICP-MS) or electrospray ionization tandem mass spectrometry (ESI-MS/MS). Separations of selenomethionine (Se-Met) and...

  • Simocyclinones: diversity of metabolites is dependent on fermentation conditions3. Schimana, J; Walker, M; Zeeck, A; Fiedler, H-P // Journal of Industrial Microbiology & Biotechnology;Sep2001, Vol. 27 Issue 3, p144 

    Simocyclinones, a novel group of angucyclinone antibiotics, are produced by Streptomyces antibioticus T� 6040. The compounds show antibacterial and antitumor properties. In submerged cultivation, the production of simocyclinones is strongly dependent on the carbon and nitrogen sources used...

  • Characterization of Water-Soluble Macromolecular Substances in Cloud Water. Feng, Jinsheng; Möller, Detlev // Journal of Atmospheric Chemistry;Jul2004, Vol. 48 Issue 3, p217 

    In this contribution we present to our knowledge the first investigations of macromolecular, often called humic-like substances (HULIS) in cloud water samples and compare them with water-soluble extracts from atmospheric PM2.5, soils and waters to study its possible origin. Chemical analysis was...

  • Quantification with HPLC–MS/MS for environmental issues: quality assurance and quality assessment. Bester, Kai // Analytical & Bioanalytical Chemistry;May2008, Vol. 391 Issue 1, p15 

    The article presents research on the quality of high-performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS) as a tool in environmental chemistry. It is noted that HPLC-MS/MS is the method of choice for quantifying polar compounds such as pesticides and...

  • HPLC and LC-MS analysis of sinomenine and its application in pharmacokinetic studies in rats. Li-hong LONG; Peng-fei WU; Xiang-long CHEN; Zui ZHANG; Yu CHEN; Yi-yong LI; You JIN; Jian-guo CHEN; Fang WANG // Acta Pharmacologica Sinica;Nov2010, Vol. 31 Issue 11, p1508 

    Aim:To improve and validate analytical methods based on HPLC and liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) for the quantitative measurement of sinomenine in rat plasma and brain tissue.Methods:The separation of analytes and the internal...

  • Metabolism and pharmacokinetics of nibentan in human blood plasma. Appolonova, S.; Valiev, V.; Dikunets, M.; Kukharenko, A.; Rodchenkov, G.; Zeltyn', E.; Bezprozvannyi, A.; Gorshkov, V.; Radzevich, A. // Pharmaceutical Chemistry Journal;Jul2010, Vol. 44 Issue 7, p341 

    n HPLC method with electrospray ionization and ion-trap mass spectral detection (LC-ESI/MSD Ion Trap SL) for determining nibentan metabolites in human blood plasma is described. The proposed method provides simultaneous determination of nibentan and its metabolites in blood plasma. Three...

  • Determination of 30 Synthetic Food Additives in Soft Drinks by HPLC/Electrospray Ionization-Tandem Mass Spectrometry. HUI GAO; MINLI YANG; MINGLIN WANG; YANSHENG ZHAO; YA CAO; XIAOGANG CHU // Journal of AOAC International;Jan/Feb2013, Vol. 96 Issue 1, p110 

    The article presents a study on a high performance liquid chromatography (HPLC)/electrospray ionization-tandem mass spectrometry (MS/MS) developed for determining synthetic food additives in soft drinks. It notes that synthetic ingredients are added to soft drinks to ensure the required...

  • Improving the Effectiveness of Method Translation for Fast and High Resolution Separations. Woodman, Michael // LC-GC Europe;Dec2006, Vol. 19 Issue 12, p41 

    The increased availability of sub-2-micron (STM) columns and increased demand for mass spectrometer friendly methods has led to a strong trend toward conversion of existing HPLC methods to smaller diameter and smaller particle size columns. While the conversion is a simple mathematical exercise...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics