Cellular Localisation of Chromogranins and Processed Products in the Diffuse Neuroendocrine System and Related Tumours

Stridsberg, Mats; Janson, Eva T.; Portela-Gomes, Guida M.
September 2004
Current Medicinal Chemistry - Immunology, Endocrine & Metabolic ;Sep2004, Vol. 4 Issue 3, p149
Academic Journal
Chromogranin A (CgA) and chromogranin B (CgB) are proteins present in secretory granules of the diffuse neuroendocrine system, with multiple pairs of basic amino acids, which are potential cleavage sites for production of biologically active peptides. We have developed antibodies against 12 defined epitopes of the CgA and 16 defined epitopes of CgB. With these, we have shown that the pancreatic neuroendocrine cells express different epitopes of the CgA and the CgB molecules. The insulin and glucagon producing cells express almost all examined epitopes of CgA and CgB, while the pancreatic polypeptide cells express some defined parts of the chromogranins and the somatostatin cells express only one CgA epitope and two CgB epitopes. Malignant endocrine pancreatic tumours show varied expression of chromogranin epitopes. The normal and neoplastic neuroendocrine cells of the gastrointestinal tract show various expressions of CgA epitopes. Thus, gastrin cells show a different expression of CgA epitopes than the enterochromaffin cells. One of the antibodies, CgA176-195, immunostained a larger cytoplasmic area than the other CgA antibodies, including the commercially available monoclonal antibody (LK2H10). In the adrenal glands, all chromaffin cells showed immunoreactivity to all region-specific chromogranin antibodies tested. By radioimmunoassay measurements we have shown that circulating plasma concentrations of different chromogranin epitopes differ between various types of tumours, indicating specific processing. We have also shown that the region-specific antibodies can be used to study biological processes. We conclude that the region-specific antibodies presented in this review are important tools to further study the biological role of chromogranins.


Related Articles

  • The Role of Chromogranin A (CgA) in Monitoring Patients with Prostate Cancer Under Androgen Deprivation Therapy: Comparison with Prostatic Specific Antigen (PSA). Bantis, Athanasios; Sountoulides, Petros; Zissimopoulos, Athanasios; Kalaitzis, Christos; Giannakopoulos, Stilianos; Agelonidou, Eleni; Pantazis, Thrasivoulos; Touloupidis, Stavros // Current Radiopharmaceuticals;May2008, Vol. 1 Issue 2, p115 

    Introduction: Neuroendocrine cells of the prostate are regulatory cells containing biogenic amines and certain neuropeptides such as chromogranin A (CgA). In the present study we evaluated the usefulness of serum CgA for monitoring prostate cancer progression. CgA levels were correlated to serum...

  • Comparative Studies of the Chromogranin A-Derived Neuropeptide WE-14. Curry, W.J.; Quinn, J.G.; Brockbank, S.; Miller, V.; McVicar, C.; Pogue, K.; Barkatullah, S.; Norlen, P. // Current Medicinal Chemistry - Immunology, Endocrine & Metabolic ;Sep2004, Vol. 4 Issue 3, p213 

    The primary amino acid sequence of WE-14, corresponding to residues 324-337 of human CgA, was deduced following purification from a liver metastasis of an ileal carcinoid and a phaeochromocytoma, respectively. WE-14 is flanked by conserved Lys-Arg proteolytic sites and its cDNA deduced sequence...

  • Pancreastatin, A Regulatory Peptide that Modulates Energy Metabolism. Sanchez-Margalet, Victor; González-Yanes, Carmen; Najib, Souad // Current Medicinal Chemistry - Immunology, Endocrine & Metabolic ;Sep2004, Vol. 4 Issue 3, p203 

    Pancreastatin (PST) is a chromogranin A (CgA)-derived peptide that was first isolated from the porcine pancreas. Although the physiological role of PST has yet to be fully established, a multitude of effects have implicated PST in the modulation of secretion and the control of energy metabolism,...

  • Aggregation, Sorting and Transport of Chromogranins in the Regulated Secretory Pathway. Gerdes, H.-H.; Rudolf, R.; Kögel, T. // Current Medicinal Chemistry - Immunology, Endocrine & Metabolic ;Sep2004, Vol. 4 Issue 3, p179 

    Chromogranin A (CgA) and chromogranin B (CgB) belong to the granin family of acidic secretory proteins with widespread distribution in neuroendocrine tissue. They are co-packaged with other proneuropeptides and prohormones into exocytotic carriers, the secretory granules, and are processed to...

  • Chromogranin A in Tumors: More Than a Marker for Diagnosis and Prognosis. Corti, Angelo; Ferrero, Elisabetta // Current Medicinal Chemistry - Immunology, Endocrine & Metabolic ;Sep2004, Vol. 4 Issue 3, p161 

    Chromogranin A (CgA), a protein stored in the secretory granules of many neuroendocrine cells and neurons, is believed to play intracellular and extracellular functions as a regulator of secretory granules biogenesis and as a precursor of various regulatory peptides of the endocrine and the...

  • Preface [Hot Topic: Chromogranin A and Its Derived Peptides as Potential Regulators of Immune, Endocrine and Metabolic Systems - In Search of Its Physiological Role (Guest Editors: K.B. Helle and G. Serck-Hanssen)]. Helle, Karen B.; Serck-Hanssen, Guldborg // Current Medicinal Chemistry - Immunology, Endocrine & Metabolic ;Sep2004, Vol. 4 Issue 3, p1 

    Introduces a series of papers about chromogranin A and its derived peptides as potential regulators of immune, endocrine and metabolic systems. Main lines of progress in the field of chromogranin research; Potential functions; Multitude of activities assigned to the proteins; Role in granule...

  • The Role of Chromogranins and Other Statins in Homeostasis: An Explanation of the Precise Regulation of Glucose and Ionised Calcium in the Blood; An Overview. Koeslag, Johan H.; Saunders, Peter T. // Current Medicinal Chemistry - Immunology, Endocrine & Metabolic ;Sep2004, Vol. 4 Issue 3, p235 

    Pairs of counter-regulatory hormones achieve very precise control of several plasma constituents. This precision implies integral control. Locally acting chromogranins and other statins within individual endocrine glands cause endocrine tissues to respond to the time integral of the error in the...

  • Gastroenteropancreatic neuroendocrine tumors: new insights in the diagnosis and therapy. Alexandraki, Krystallenia; Kaltsas, Gregory // Endocrine (1355008X);Feb2012, Vol. 41 Issue 1, p40 

    Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) are relatively rare and heterogenous malignancies. Recent advances in histopathological classification according to the anatomical site of origin, proliferation rate, and extend of the disease have created a valid and powerful tool for the...

  • Elevated chromogranin A serum levels in ovarian carcinoma patients. Malaguarnera, M.; Uccello, M.; Bellanca, S.; La Rosa, B.; Vacante, M.; Cristaldi, E.; Biondi, A.; Basile, F.; Malaguarnera, L. // Indian Journal of Cancer;Jul-Sep2014, Vol. 51 Issue 3, p315 

    BACKGROUND: The observation of neuroendocrine activity during clinical course of ovarian cancer, suggested the use of neuroendocrine serum markers to detect this tumor. AIM: To evaluate the usefulness of serum measurements of chromogranin A (CgA) in the various stages of ovarian cancer....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics