Specific polyclonal-based immunoassays for sulfathiazole

Pastor-Navarro, Nuria; García-Bover, Cristina; Maquieira, Ángel; Puchades, Rosa
August 2004
Analytical & Bioanalytical Chemistry;Aug2004, Vol. 379 Issue 7/8, p1088
Academic Journal
A highly sensitive and specific enzyme-linked immunosorbent assay has been developed for detection of sulfathiazole (STZ, 4-amino-N-thiazol-2-yl-benzene-sulfonamide). A set of haptens was synthesized in order to produce polyclonal antibodies against sulfonamides. Two ELISA formats (antibody-coated and conjugate-coated) were also investigated using all the serum/coating conjugate combinations that showed specific recognition. The developed ELISA succeeded in detection of STZ at concentrations as low as 0.03 ng mL-1 over a measurable range of 0.12-6.71 ng mL-1. Selectivity studies have demonstrated that other sulfonamides do not interfere significantly (<10%) with analysis of STZ by this immunochemical technique. Analysis of spiked bee honey samples by the developed ELISA method showed recoveries were good. The selectivity and sensitivity (IC50 = 1.6 ng mL-1) make it a suitable screening method for determination of low levels of STZ in food samples.


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