TITLE

Specific polyclonal-based immunoassays for sulfathiazole

AUTHOR(S)
Pastor-Navarro, Nuria; García-Bover, Cristina; Maquieira, Ángel; Puchades, Rosa
PUB. DATE
August 2004
SOURCE
Analytical & Bioanalytical Chemistry;Aug2004, Vol. 379 Issue 7/8, p1088
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A highly sensitive and specific enzyme-linked immunosorbent assay has been developed for detection of sulfathiazole (STZ, 4-amino-N-thiazol-2-yl-benzene-sulfonamide). A set of haptens was synthesized in order to produce polyclonal antibodies against sulfonamides. Two ELISA formats (antibody-coated and conjugate-coated) were also investigated using all the serum/coating conjugate combinations that showed specific recognition. The developed ELISA succeeded in detection of STZ at concentrations as low as 0.03 ng mL-1 over a measurable range of 0.12-6.71 ng mL-1. Selectivity studies have demonstrated that other sulfonamides do not interfere significantly (<10%) with analysis of STZ by this immunochemical technique. Analysis of spiked bee honey samples by the developed ELISA method showed recoveries were good. The selectivity and sensitivity (IC50 = 1.6 ng mL-1) make it a suitable screening method for determination of low levels of STZ in food samples.
ACCESSION #
14281866

 

Related Articles

  • Assessment of an ELISA Laboratory Exercise. Robinson, David L.; Lau, Joann M. // American Biology Teacher (University of California Press);Oct2012, Vol. 74 Issue 8, p558 

    The enzyme-linked immunosorbent assay (ELISA) is a powerful immunological technique for quantifying small amounts of compounds and has been used in research and clinical settings for years. Although there are laboratory exercises developed to introduce the ELISA technique to students, their...

  • Improvement in sensitivity of enzyme-linked immunosorbent assay for tumour necrosis factor. McLaughlin, P. Jeremy; Elwood, Ngaire J.; Ramadi, Lanny T.; Pica, Marie R.; McKenzie, Ian F. C. // Immunology & Cell Biology;Feb1990, Vol. 68 Issue 1, p51 

    Various protocols were used in the development of enzyme-linked immunosorbent assays (ELISA) to improve the sensitivity and range of detection of human tumour necrosis factor-α (TNF-α). ELISA can provide a specific, sensitive and rapid method for detection of TNF-α in patient's sera,...

  • Evaluation of a polyclonal antiserum to pentachlorothiophenol-acetic acid-KLH immunogen: binding properties and use with heterologous PCP derivatives in ELISA for pentachlorophenol. Abuknesha, Ramadan A.; Griffith, Hannah M. T. // Analytical & Bioanalytical Chemistry;Jun2004, Vol. 379 Issue 3, p411 

    A polyclonal antiserum to pentachlorothiophenol-acetic acid-KLH was generated in sheep and assessed by solid phase ELISA. The assessment procedure included use of double checkerboard analysis in the absence and in the presence of analyte loads, estimation of cross reactivities of chlorophenol...

  • A Reliable Way to Detect Endogenous Murine β-Amyloid. Teich, Andrew F.; Patel, Mitesh; Arancio, Ottavio // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    Unraveling the normal physiologic role of β-amyloid is likely crucial to understanding the pathogenesis of Alzheimer's disease. However, progress on this question is currently limited by the high background of many ELISAs for murine β-amyloid. Here, we examine the background signal of...

  • Multivariate analysis to separate the signal given by cross-reactants in immunoassay with sample matrix dilution. Nistor, Catalin; Christensen, Jakob; Ocio, Natalia; Nørgaard, Lars; Emnéus, Jenny // Analytical & Bioanalytical Chemistry;Dec2004, Vol. 380 Issue 7/8, p898 

    This paper describes a new approach to achieve selectivity in an immunoassay by separating the signals given by two cross-reactive compounds present simultaneously in a complex sample matrix. The method is based on the sequential dilution of the sample containing a mixture of the two analytes,...

  • Adventures in Immunoassay. Hurst, Jeff // Scientific Computing & Instrumentation;Feb2001, Vol. 18 Issue 3, p12 

    Discusses the replacement of radioimmunoassay-based techniques with enzyme linked immunosorbent assay (ELISA) methods in immunoassay technology in the clinical sector. Function of ELISA in medical applications; Benefits and disadvantages of ELISA methods; Developments in the technology.

  • Immunoassay to detect enhanced carbamothioate degradation in soil Shea, Patrick J.; Takacs, James M.; Staswick, Paul E.; Hutchinson, Pamela J. // Weed Technology;Apr/Jun1993, Vol. 7 Issue 2, p396 

    No abstract available.

  • Analysis of the Kanamycin in Raw Milk Using the Suspension Array. Yanfei Wang; Mingqiang Zou; Yi Han; Fan Zhang; Jinfeng Li; Xiaoyan Zhu // Journal of Chemistry;2013, p1 

    With the monoclonal antibody against kanamycin being prepared successfully, a bead-based indirect competitive fluorescent immunoassay was developed to detect kanamycin in milk. The fact that there was no significant cross-reaction with other aminoglycoside antibiotics implied that the monoclonal...

  • Serogrupos, serosubtipos, inmunotipos y susceptibilidad antimicrobiana de cepas de Neisseria meningitidis aisladas de portadores. Zamora, Leydis; Martínez, Isabel; Gutiérrez, Mercedes; Núñez, Niuris; Ginebra, Mónica; Climent, Yanet; Sotolongo, Franklin; Camaraza, María Amalia // Revista VacciMonitor (Vacunología y Temas Afines);Jan2002, Vol. 11 Issue 1, p1 

    Considering the fundamental role played by asymptomatic Neisseria meningitidis carriers in the transmission of the meningococcal disease, it is necessary to study the issues related to the phenotypical characterization of this microorganism more deeply. With the purpose of contributing to the...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics