TITLE

Genotyping of Three Single Nucleotide Polymorphisms Following Whole Genome Preamplification of DNA Collected from Buccal Cells

AUTHOR(S)
Haaberstick, Brett C.; Smolen, Andrew
PUB. DATE
September 2004
SOURCE
Behavior Genetics;Sep2004, Vol. 34 Issue 5, p541
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Collection of genomic DNA from buccal cells is a simple and convenient procedure for genotyping individuals. One disadvantage is that the amount of genomic DNA may be inadequate for genotyping projects that require a large number of determinations per sample. Primer Extension Preamplification (PEP) methods that can amplify the entire genome 100-fold or more offer a potential solution to this problem. To test whether the preamplified DNA product retains the original allelic distribution of the genomic DNA sample from which it was derived, we compared PEP buccal DNA with genomic buccal DNA from 94 pairs of monozygotic twins. Using the 5′ nuclease (Taqman®) method, we genotyped three SNPs that are of wide interest in behavioral and psychiatric genetic studies. The percent agreement between allele calls using PEP DNA samples and those using genomic DNA samples ranged between 99% and 100% depending on SNP assay. These data complement our earlier findings with respect to microsatellite markers and variable number tandem repeat (VNTR) polymorphisms and indicate that whole-genome preamplification is an appropriate method for providing DNA for SNP genotyping these and possibly other loci.
ACCESSION #
14205193

 

Related Articles

  • What's in the 'junk' of the genome? Kiermer, Veronique // Nature Methods;May2005, Vol. 2 Issue 5, p328 

    A large-scale analysis of the human transcriptome with unprecedented resolution raises the possibility that large numbers of transcripts are produced from regions of the genome considered as intergenic in the current annotation.

  • Chemical Genomics Is the "Big Tent" of Drug Discovery. DePalma, Angelo // Drug Discovery & Development;Oct2004, Vol. 7 Issue 10, p51 

    Discusses the significance of chemical genomics in drug discovery. Popularity of classical chemical genomics; Terminologies related to chemical genomics; Detection of targets for drugs rather than drugs for targets; Screening of proteins; Fragment-based approach. INSETS: Back to Nature;NH Funds...

  • Expression profiling identifies novel candidate genes for ethanol sensitivity QTLs. MacLaren, Erik J.; Bennett, Beth; Johnson, Thomas E.; Sikela, James M. // Mammalian Genome;Feb2006, Vol. 17 Issue 2, p147 

    The Inbred Long Sleep (ILS) and Inbred Short Sleep (ISS) mouse strains have a 16-fold difference in duration of loss of the righting response (LORR) following administration of a sedative dose of ethanol. Four quantitative trait loci (QTLs) have been mapped in these strains for this trait....

  • COMT Gene Polymorphism Is Associated with Declarative Memory in Adulthood and Old Age. De Frias, Cindy M.; Annerbrink, Kristina; Westberg, Lars; Eriksson, Elias; Adolfsson, Rolf; Nilsson, Lars-Göran // Behavior Genetics;Sep2004, Vol. 34 Issue 5, p533 

    Variation in memory performance is to a large extent explained by genes. In the prefrontal cortex, the catechol O-methyltransferase (COMT) gene is essential in the metabolic degradation of dopamine, a neurotransmitter implicated in cognitive functions. The present study examined the effect of a...

  • Pharmacogenetics: data, concepts and tools to improve drug discovery and drug treatment. Brockmöller, Jürgen; Tzvetkov, Mladen V. // European Journal of Clinical Pharmacology;Feb2008, Vol. 64 Issue 2, p133 

    Variation in the human genome is a most important cause of variable response to drugs and other xenobiotics. Susceptibility to almost all diseases is determined to some extent by genetic variation. Driven by the advances in molecular biology, pharmacogenetics has evolved within the past 40 years...

  • Pharmacogenetics and pharmacogenomics: recent developments, their clinical relevance and some ethical, social, and legal implications. Paul, Norbert W.; Roses, Allen D. // Journal of Molecular Medicine;Mar2003, Vol. 81 Issue 3, p135 

    In recent debates on novel procedures of molecular medicine pharmacogenomics is attracting more and more attention as a genotype-based approach for improving safety and efficacy of the use of therapeutic substances. Promoted by basic knowledge generated in the field of medical genomics,...

  • PARASITOSIS from A to T. KOLCHANOV, N. A.; MORDVINOV, V. A. // SCIENCE First Hand;2008, Vol. 19 Issue 1, p22 

    The article discusses the state of genomic research in Russia. Inspite of the range of scales of genome sequencing projects worldwide aimed at decoding genomes of various complexities, Russia is stated to be unfortunately behind in this trend. It is hoped that the large-scale project aimed at a...

  • Diversity within the genus Elymus (Poaceae: Triticeae) II: analyses of variation within 5S nrDNA restrict membership in the genus to species with StH genomes. Baum, Bernard; Edwards, Tara; Johnson, Douglas // Molecular Genetics & Genomics;Feb2016, Vol. 291 Issue 1, p217 

    The genus Elymus is a repository for a large number of species that have been difficult to classify by traditional techniques due to their remarkable levels of polymorphism. Following the genome analyses of Yen and Yang (Genus Elymus 5:58-362, ), we used sequences of the nr5SDNA to investigate...

  • Single-cell genomics. Walker, Alan; Parkhill, Julian // Nature Reviews Microbiology;Mar2008, Vol. 6 Issue 3, p176 

    Efforts to describe and functionally categorize the amazing diversity of microbial life have long been hampered by our inability to cultivate most microorganisms in the laboratory. Recent technological advances in whole-genome amplification and cell sorting now permit insights into previously...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics