微小 RNA-30e 调控EMT对胃癌侵袭和迁移影响的研究

章萧; 艾芬; 张莉红; 刘迪; 张佩雯
November 2019
Progress in Modern Biomedicine;Nov2019, Vol. 19 Issue 22, p4208
Academic Journal
Objective: To investigate the effect of microRNA-30e (miR-30e) on the invasion and migration of gastric cancer and the potential mechanism. Mrthods: The transwell system and the assays of wound healing were used to assess the invasion and migration ability of gastric cell line BGC823. The miR-30e was transfected into BGC823 cell via lipofectamine 2000. Cells were also transfected with empty vectors to serve as controls. The expression of miR-30e was detected by real-time PCR, and western blot was used to assay the expression of Snail, Vimentin, N-cadherin, and E-cadherin which were the markers of epithelial-mesenchymal transition EMT. Results: After the transfection of the miR-30e, which had significantly suppressed the major factors in EMT signaling pathway, such as the expression of mRNA and proteins of Snail, N-cadherin, and vimentin, increased the mRNA and proteins expression of E-cadherin. And the mimic also had suppressed the invasion and migration of BGC823 cell line induced by TGF-β. Conclusion: MiR-30e may be a key target in the EMT process of cancer cells, blocking the EMT process could inhibit the invasion and migration of gastric cancer cells.


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