TITLE

Altered glucose metabolism and proteolysis in pancreatic cancer cell condition!d myoblasts: searching for a gene expression pattern with a microarray analysis of 5000 skeletal muscle genes

AUTHOR(S)
Basso, D.; Millino, C.; Greco, E.; Romualdi, C.; Fogar, P.; Valerio, A.; Bellin, M.; Zambon, C. -F.; Navaglia, F.; Dussini, N.; Avogaro, A.; Pedrazzoli, S.; Lanfranchi, G.; Plebani, M.
PUB. DATE
August 2004
SOURCE
Gut;Aug2004, Vol. 53 Issue 8, p1159
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background and aims: We verified whether conditioned media (CM) from pancreatic cancer cell lines (MIAPaCa2, CAPAN-1, PANC-1, BxPC3) alter glucose metabolism and gene expression profiles (microarray experiment with a platform of 5000 skeletal muscle cDNA) in mice myoblasts. Methods: Myoblasts were incubated with control or pancreatic cancer CM for 24 and 48 hours. Results: Lactate significantly increased in CM compared with non-conditioned myoblasts. No variations in expression levels of the main genes involved in glycolysis were found in CM myoblasts. Propionyl coenzyme A carboxylase and isocitrate dehydrogenase 3 beta genes, which encode enzymes of the tricarboxylic acid cycle, were overexpressed, while IGFIIR and VAMP5 genes were underexpressed in CM myoblasts. PAFAH1B1 and BCL-2 genes (intracellular signal transduction) and the serine protease cathepsin G (proteolysis), were overexpressed in CM myoblasts. Tyrosine accumulation in CM myoblasts suggested that proteolysis overcomes protein synthesis. Sorcin, actin alpha, troponin Ti, and filamin A were underexpressed in CM myoblasts. Conclusions: Our findings demonstrate that pancreatic cancer cell conditioned media enhanced lactate production and induced proteolysis, possibly by altering expression levels of a large number of genes, not only those involved in protein biosynthesis and degradation or glucose metabolism, but also those involved in the tricarboxylic acid cycle and in vesicle traffic.
ACCESSION #
13986670

 

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