TITLE

Biodistribution of nanodiamonds in the body of mice using EPR spectrometry

AUTHOR(S)
Inzhevatkin, Evgeny; Baron, Alexey; Maksimov, Nikolai; Volkova, Marina; Puzyr, Alexey; Ronzhin, Nikita; Bondar, Vladimir
PUB. DATE
September 2019
SOURCE
IET Science, Measurement & Technology;2019, Vol. 13 Issue 7, p984
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In vitro experiments proved the usefulness of electron paramagnetic resonance (EPR) spectrometry for detecting detonation nanodiamonds (NDs) in samples of biomaterials (blood and homogenates of organs of mice). A characteristic EPR signal (g = 2.003, ΔH ≃ 10 G) was detected in biomaterials containing NDs, and its intensity linearly increased at nanoparticle concentrations of between 1.6 and 200 μg/ml. In vivo experiments demonstrated that EPR spectrometry was effective for monitoring the inter-organ distribution of NDs intravenously injected to mice. In 2.5 h after the injection of NDs, the nanoparticles mainly accumulated in the lungs and liver of the animals - about 25 and 20%, respectively, of the initially injected NDs. The amounts of NDs accumulated in the heart and kidneys were considerably lower. Also, EPR spectrometry did not detect NDs in the blood, spleen, brain, and femoral muscles of mice. Ten days after injection, EPR spectrometry detected redistribution of NDs in mice. The amounts of nanoparticles decreased approximately by a factor of 3.5 in the lungs and increased almost by a factor of 3 in the liver; NDs were detected in the spleen. This study suggests ways to use EPR spectrometry to study the distribution, accumulation, and elimination of detonation NDs injected into laboratory animals.
ACCESSION #
138286454

 

Related Articles

  • Reduction of Image Artifacts in Mice by Bladder Flushing with a Novel Double-Lumen Urethral Catheter. Haney, Chad R.; Parasca, Adrian D.; Ichikawa, Kazuhiro; Williams, Benjamin B.; Elas, Martyna; Pelizzari, Charles A.; Halpern, Howard J. // Molecular Imaging;Jul2006, Vol. 5 Issue 3, p175 

    In electron paramagnetic resonance imaging (EPRI), the accumulation of contrast agent in the bladder can create a very large source of signal, often far greater than that of the organ of interest. Mouse model images have become increasingly important in preclinical testing. To minimize bladder...

  • Dose dependences of lipid microviscosity of biological membranes induced by synthetic antioxidant potassium phenosan salt. Palmina, N.; Chasovskaya, T.; Belov, V.; Maltseva, E. // Doklady Biochemistry & Biophysics;Apr2012, Vol. 443 Issue 1, p100 

    The article presents a study which investigates the effect of potassium phenosan salt on the lipid microviscosity of electron paramagnetic resonance (EPR) and plasma membrane (PM) of hepatocytes. The study used potassium phenosan solutions with 100 F1 mice by diluting the initial solution with...

  • Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy. Ito, Shinobu; Mori, Tomohisa; Kanazawa, Hideko; Sawaguchi, Toshiko // Journal of Toxicology;2011, p1 

    Electron spin resonance (ESR) method is a simplemethod for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to...

  • Smoking Induces Bimodal DNA Damage in Mouse Lung. Ueno, Shunji; Kashimoto, Takashige; Susa, Nobuyuki; Ishikawa, Masaaki; Kawagoe, Tadahiro; Mizuta, Kenji; Nishimura, Masaaki; Homma-Takeda, Shino; Temma, Kyosuke // Toxicological Sciences;Apr2011, Vol. 120 Issue 2, p322 

    To clarify the relationship between DNA damage and free radical generation caused by smoking in vivo, DNA damage was investigated in the mouse lung by single-cell gel electrophoresis assay after exposure to cigarette smoke (CS) or gas phase cigarette smoke (GPCS). Although GPCS did not induce...

  • Application of Electron Paramagnetic Resonance (EPR) Oximetry to Monitor Oxygen in Wounds in Diabetic Models. Desmet, Céline M.; Lafosse, Aurore; Vériter, Sophie; Porporato, Paolo E.; Sonveaux, Pierre; Dufrane, Denis; Levêque, Philippe; Gallez, Bernard // PLoS ONE;12/14/2015, Vol. 10 Issue 12, p1 

    A lack of oxygen is classically described as a major cause of impaired wound healing in diabetic patients. Even if the role of oxygen in the wound healing process is well recognized, measurement of oxygen levels in a wound remains challenging. The purpose of the present study was to assess the...

  • Schottky Mass Spectrometry at the ESR with a new data acquisition system. Falch, M.; Lo¨bner, K. E. G.; Kerscher, Th.; Attallah, F.; Bosch, F.; Franzke, B.; Geissel, H.; Hausmann, M.; Klepper, O.; Kluge, H.; Kozhuharov, C.; Mu¨nzenberg, G.; Nolden, F.; Novikov, Y.; Patyk, Z.; Radon, T.; Scheidenberger, C.; Steck, M.; Winkler, M.; Wollnik, H. // AIP Conference Proceedings;2000, Vol. 512 Issue 1, p263 

    Schottky Mass Spectrometry turned out to be an excellent tool for systematic nuclear mass measurements. The newly developed data acquisition system [1] which yields several advantages for the data analysis, was used for the first time in Nov. 1997. Instead of online Fourier transformation as in...

  • Spontaneous skin damage and delayed wound healing in SOD1-deficient mice. Iuchi, Yoshihito; Roy, Dipa; Okada, Futoshi; Kibe, Noriko; Tsunoda, Satoshi; Suzuki, Saori; Takahashi, Motoko; Yokoyama, Hidekatsu; Yoshitake, Jun; Kondo, Seiji; Fujii, Junichi // Molecular & Cellular Biochemistry;Aug2010, Vol. 341 Issue 1/2, p181 

    Superoxide dismutase 1 (SOD1) is an important antioxidative enzyme that protects skin from oxidative stress. SOD1−/− mice with a genetic background of b129Sv mice showed facial skin damage after 15 weeks of age. Eyelid swelling occurred as the initial symptom and caused impairment by...

  • First Combined In Vivo X-Ray Tomography and High-Resolution Molecular Electron Paramagnetic Resonance (EPR) Imaging of the Mouse Knee Joint Taking into Account the Disappearance Kinetics of the EPR Probe. Bézière, Nicolas; Decroos, Christophe; Mkhitaryan, Karen; Kish, Elizabeth; Richard, Frédéric; Bigot-Marchand, Stéphanie; Durand, Sylvain; Cloppet, Florence; Chauvet, Caroline; Corvol, Marie-Thérèse; Rannou, François; Yun Xu-Li; Mansuy, Daniel; Peyrot, Fabienne; Frapart, Yves-Michel // Molecular Imaging;May/Jun2012, Vol. 11 Issue 3, p220 

    Although laboratory data clearly suggest a role for oxidants (dioxygen and free radicals derived from dioxygen) in the pathogenesis of many age-related and degenerative diseases (such as arthrosis and arthritis), methods to image such species in vivo are still very limited. This methodological...

  • Effect of sage and garlic on lipid oxidation in high-pressure processed chicken meat. Lilian Mariutti; Vibeke Orlien; Neura Bragagnolo; Leif Skibsted // European Food Research & Technology;Jun2008, Vol. 227 Issue 2, p337 

    Abstract  Sage was found to protect minced chicken breast processed with high hydrostatic pressure up to 800 MPa for 10 min against lipid oxidation during subsequent chilled storage for 2 weeks. Garlic showed prooxidative effects especially at moderate high pressure around...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics