Mobile Phase Buffers in LC: Effect of Buffer Preparation Method on Retention Repeatability

Stoll, Dwight R.; Makey, Devin M.
July 2019
LC-GC North America;Jul2019, Vol. 37 Issue 7, p444
Trade Publication
The measurement of pH, one of the most common of all analytical measurements, plays a major role in many chemical processes, affecting everything from the productivity of bioreactors in the biopharmaceutical industry, to the performance of separation methods in liquid chromatography (LC) and electrophoresis. Given that pH measurement is so common, I think we can be lulled into the perception that it is also simple, and that the pH reported by any benchtop pH meter can be accepted at face value under all circumstances. In my interactions working with people at a variety of experience levels over the years, I have often felt that people preparing buffers for use in LC are a little too trusting of the pH values reported by pH meters under ordinary circumstances. In preparation for this month's "LC Troubleshooting" column, my student Devin Makey and I performed some experiments to see if we could move in the direction of getting answers to questions around the topic of the "best" way to prepare buffers for use in LC. What follows here is a description of those experiments, and the data we observed. I believe that the results are interesting, and can support best practices for improving the reliability of LC methods. I know they are certainly affecting the way we operate in my laboratory, and I hope you will find them useful as well.


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