TITLE

Influence of Strontium on Vascular Endothelial Growth Factor and Fibroblast Growth Factor 2 Expression in Rat Chondrocytes Cultured In Vitro

AUTHOR(S)
Zhu, Xiaoyan; Kong, Yezi; Huang, Yan; Zhao, Baoyu; Wang, Jianguo
PUB. DATE
August 2019
SOURCE
Biological Trace Element Research;Aug2019, Vol. 190 Issue 2, p466
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Strontium (Sr) can reduce cartilage degeneration and stimulate cartilage matrix formation. Angiogenesis plays a developmental role in chondrogenesis, and was stimulated by growth factors such as vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF2). However, the direct influence of Sr on VEGF and FGF2 expressions in chondrocytes is not entirely clear. The objective of this study was to investigate the effects of different Sr concentrations on VEGF and FGF2 expressions in rat chondrocytes in vitro. Chondrocytes were isolated from Wistar rat articular by enzymatic digestion. As a Sr source, strontium chloride hexahydrate (SrCl2·6H2O) was added to the culture solution at final concentrations of 0, 0.5, 1.0, 2.0, 5.0, 20.0, and 100.0 μg/mL. After 72 h of continuous culture, mRNA abundance and protein expression levels of VEGF and FGF2 in the chondrocytes were determined by real-time polymerase chain reaction (real-time PCR) and Western blot, respectively. The results showed that VEGF and FGF2 expressions were dose-dependently elevated with Sr concentration in chondrocytes. The mRNA abundance and protein expression levels of VEGF were extremely significantly higher than those in the control group (P < 0.01) at 1.0 μg/mL Sr treatment. For FGF2, there were markedly significant differences in mRNA and protein expression from control group (P < 0.01) when the Sr-treated concentration exceeded 5.0 μg/mL and 20.0 μg/mL, respectively. These results indicated that Sr might involve in the cartilage angiogenesis via regulating expression of VEGF and FGF2z.
ACCESSION #
137251743

 

Related Articles

  • HMGB1 Protects the Heart Against Ischemia–Reperfusion Injury via PI3K/AkT Pathway-Mediated Upregulation of VEGF Expression. Zhou, Yan-Hong; Han, Qian-Feng; Gao, Lei; Sun, Ying; Tang, Zhan-Wei; Wang, Meng; Wang, Wei; Yao, Heng-Chen // Frontiers in Physiology;1/29/2020, p1 

    Delivery of exogenous high mobility group box 1 (HMGB1) may exert a beneficial effect on myocardial ischemia–reperfusion (I/R) injury. Since the expression of vascular endothelial growth factor (VEGF) and phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) in the myocardium mediates...

  • Effects of occlusal hypofunction and its recovery on PDL structure and expression of VEGF and bFGF in rats. Motokawa, M.; Kaku, M.; Matsuda, Y.; Kojima, S.; Sumi, C.; Kimura, A.; Tanimoto, K. // Clinical Oral Investigations;May2015, Vol. 19 Issue 4, p929 

    Objectives: The purpose of this study was to clarify whether occlusal hypofunction and its recovery affect the structure of the periodontal ligament (PDL) and expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in rats. Materials and methods:...

  • Effect of Local Basic Fibroblast Growth Factor and Vascular Endothelial Growth Factor on Subcutaneously Allotransplanted Ovarian Tissue in Ovariectomized Mice. Gao, Jiangman; Huang, Ying; Li, Min; Zhao, Hongcui; Zhao, Yue; Li, Rong; Yan, Jie; Yu, Yang; Qiao, Jie // PLoS ONE;7/24/2015, Vol. 10 Issue 7, p1 

    Objective: One of the major obstacles to ovarian tissue preservation is delayed angiogenesis that leads follicles lost after transplantation. The aim of the present study was to investigate the effects of bFGF and VEGF on heterotopic transplanted ovarian tissue using a mouse model. Methods: ...

  • Synergistic and sequential effects of BMP-2, bFGF and VEGF on osteogenic differentiation of rat osteoblasts. Li, Peipei; Bai, Yan; Yin, Guangfu; Pu, Ximing; Huang, Zhongbing; Liao, Xiaoming; Chen, Xianchun; Yao, Yadong // Journal of Bone & Mineral Metabolism;Nov2014, Vol. 32 Issue 6, p627 

    In the present study, the effects of bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on regulation of rat osteoblast (ROB) maturation in vitro were investigated. It was found that the proliferation, differentiation and...

  • Fingolimod Increases Brain-Derived Neurotrophic Factor Level Secretion from Circulating T Cells of Patients with Multiple Sclerosis. Golan, Maya; Mausner-Fainberg, Karin; Ibrahim, Bassima; Benhamou, Moshe; Wilf-Yarkoni, Adi; Kolb, Hadar; Regev, Keren; Karni, Arnon // CNS Drugs;Dec2019, Vol. 33 Issue 12, p1229 

    Background: The pathophysiology of multiple sclerosis involves an autoimmune and a neurodegenerative mechanism. Central nervous system-infiltrating immune cells in multiple sclerosis also possess a neuroprotective activity through secretion of neurotrophins, such as brain-derived...

  • Effects of Transplantation of Human Cord Blood Mononuclear Cells Expressing the Recombinant VEGF and FGF2 Genes into Spinal Cord Traumatic Injury Sites in Rats. Shaimardanova, G.; Mukhamedshina, Ya.; Rizvanov, A.; Salafutdinov, I.; Chelyshev, Yu. // Neuroscience & Behavioral Physiology;Jun2013, Vol. 43 Issue 5, p597 

    A model of dosed TVIII spinal cord contusion trauma in rats was used to study the effects of immediate single-dose transplantation of human cord blood mononuclear cells transformed with the recombinant genes for neurotrophic factors - vascular endothelia growth factor (VEGF) and fibroblast...

  • Update on the treatment of androgenetic alopecia. RINALDI, S.; BUSSA, M.; MASCARO, A. // European Review for Medical & Pharmacological Sciences;Jan2016, Vol. 20 Issue 1, p54 

    OBJECTIVE: Androgenetic alopecia is the most common type of hair loss, affecting women (50% of menopausal women and a large number of women of childbearing age) as well as males (over 70% of adult men). Since the condition is of an evolutionary nature, it is important to intervene early in order...

  • In vitro and in vivo affinity microdialysis sampling of cytokines using heparin-immobilized microspheres. Jia Duo; Stenken, Julie A. // Analytical & Bioanalytical Chemistry;Jan2011, Vol. 399 Issue 2, p783 

    Heparin-immobilized microspheres were included in microdialysis sampling perfusion fluids under both in vitro and in vivo conditions to improve the recovery of different cytokines, acidic fibroblast growth factor, vascular endothelial growth factor, monocyte chemoattractant protein-1 (or CCL2),...

  • Alternative Splicing and Cancer. Auboeuf, Didier; Carmo-Fonseca, Maria; Valcarcel, Juan; Biamonti, Giuseppe // Journal of Nucleic Acids;2012, p1 

    An introduction is presented in which the editor discusses various reports within the issue on topics including splicing alteration in cancer, alternative splicing of vascular endothelial growth factor (VEGF) and splicing alterations of fibroblast growth factor receptors (FGFR).

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics