TITLE

BioSuiteâ„¢ Peptide Analysis Columns: Importance of Peak Capacity on Complex Peptide Separations

AUTHOR(S)
Gilar, Martin; Daly, Amy E.; Gebler, John C.
PUB. DATE
May 2004
SOURCE
LC-GC Europe;May2004 Applications, Vol. 17, p19
SOURCE TYPE
Trade Publication
DOC. TYPE
Article
ABSTRACT
Complex peptide samples generated by protein digestion may contain tens to hundreds of components. Separations are typically performed In RP-HPLC mode under gradient conditions. Given the complexity of many digests, highly efficient HPLC columns are required for successful separations. When using gradient elution, the separation efficiency of the column is best described by column peak capacity. Peak capacity is defined as the maximum number of peaks that can be separated within a gradient time. State-of-the-art RP-HPLC columns have peak capacities of 100-300, depending an column length and the particle size of the sorbent. This article evaluates the peak capacity of several columns using a 9 peptide standard mixture and a tryptic digest of yeast enolase.
ACCESSION #
13523567

 

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics