Life still goes on without 'vital' DNA

Westphal, Sylvia Pagán
June 2004
New Scientist;6/5/2004, Vol. 182 Issue 2450, p18
Recently researchers revealed that they had deleted huge chunks of the genome of mice without it making any discernable difference to the animals. All DNA tends to acquire random mutations, but if these occur in a region that has an important function, individuals will not survive. Key sequences should thus remain virtually unchanged, even between species. So by comparing the genomes of different species and looking for regions that are conserved, geneticists hope to pick out those that have an important function. To find out the function of some of these highly conserved non-protein-coding regions in mammals, Edward Rubin's team at the Lawrence Berkeley National Laboratory in California deleted two huge regions of junk DNA from mice containing nearly 1000 highly conserved sequences shared between human and mice.


Related Articles

  • Induction of recombination between diverged sequences in a mammalian genome by a double-strand break. Bhattacharjee, Vikram; Lin, Yunfu; Waldman, Barbara; Waldman, Alan // Cellular & Molecular Life Sciences;Jun2014, Vol. 71 Issue 12, p2359 

    To investigate whether mammalian cells can carry out recombinational double-strand break (DSB) repair between highly diverged sequences, mouse fibroblasts were transfected with DNA substrates that contained a 'recipient' thymidine kinase ( tk) gene disrupted by the recognition site for...

  • Maintaining genome stability at the replication fork. Branzei, Dana; Foiani, Marco // Nature Reviews Molecular Cell Biology;Mar2010, Vol. 11 Issue 3, p208 

    Aberrant DNA replication is a major source of the mutations and chromosome rearrangements that are associated with pathological disorders. When replication is compromised, DNA becomes more prone to breakage. Secondary structures, highly transcribed DNA sequences and damaged DNA stall replication...

  • MamPhEA: a web tool for mammalian phenotype enrichment analysis. Weng, Meng-Pin; Liao, Ben-Yang // Bioinformatics;Sep2010, Vol. 26 Issue 17, p2212 

    Summary: MamPhEA is a web application dedicated to understanding functional properties of mammalian gene sets based on mouse-mutant phenotypes. It allows users to conduct enrichment analysis on predefined or user-defined phenotypes, gives users the option to specify phenotypes derived from null...

  • Decoding cell lineage from acquired mutations using arbitrary deep sequencing. Carlson, Cheryl A; Kas, Arnold; Kirkwood, Robert; Hays, Laura E; Preston, Bradley D; Salipante, Stephen J; Horwitz, Marshall S // Nature Methods;Jan2012, Vol. 9 Issue 1, p78 

    Because mutations are inevitable, the genome of each cell in a multicellular organism becomes unique and therefore encodes a record of its ancestry. Here we coupled arbitrary single primer PCR with next-generation DNA sequencing to catalog mutations and deconvolve the phylogeny of cultured mouse...

  • Taxon-specific regulation of the SOS response in γ-proteobacteria. Sycheva, L. V.; Permina, E. A.; Gelfand, M. S. // Molecular Biology;Sep2007, Vol. 41 Issue 5, p827 

    The SOS response is a cascade of consecutive reactions induced by cell DNA damage. The genes directly involved in these reactions are regulated by LexA, which binds to specific nucleotide sequences in their upstream regions. The presence of such a sequence in the regulatory gene region can be...

  • EXPRESSION PATTERNS OF PROTEINS INVOLVED IN DNA DOUBLE STRAND BREAK REPAIR IN GASTRIC CANCER. Grabsch, H.; Rotimi, O.; Agarwal, M.; Mueller, W. // Gut;Apr2004 Supplement 3, Vol. 53, pA15 

    The mammalian genome is at constant risk of mutation as a result of continually being exposed to DNA damaging agents. One particularly harmful form of DNA damage is the double strand break (DSB). DSBs repair depends on Iwo distinct mechanisms:homologous recombination (HR) and nonhomologous...

  • Transcription Initiation Activity Sets Replication Origin Efficiency in Mammalian Cells. Sequeira-Mendes, Joana; Díaz-Uriarte, Ramón; Apedaile, Anwyn; Huntley, Derek; Brockdorff, Neil; Gómez, María // PLoS Genetics;Apr2009, Vol. 5 Issue 4, p1 

    Genomic mapping of DNA replication origins (ORIs) in mammals provides a powerful means for understanding the regulatory complexity of our genome. Here we combine a genome-wide approach to identify preferential sites of DNA replication initiation at 0.4% of the mouse genome with detailed...

  • Searching for mismatches in a vast genomic landscape. Pastrana, Erika // Nature Methods;Jul2010, Vol. 7 Issue 7, p492 

    The article discusses changes in genomes. It was generally accepted that genome remains unchanged throughout one's life. It has been discovered that certain enzymes in cells can change RNA and DNA sequence. It highlights a research conducted by Alexander Wait Zaranek and Erez Levanon on genomes....

  • Draft mouse genome makes public debut. Check, Erika // Nature;5/9/2002, Vol. 417 Issue 6885, p106 

    Announces the draft assembly of mouse genome by the Mouse Genome Sequencing Consortium in the U.S. Importance of the mouse genome in biomedical research; Identification of mouse variants associated with phenotypes; Plans of Celera in publishing mouse genome.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics