TITLE

Proteinuria in rats induced by serum from patients with collapsing glomerulopathy

AUTHOR(S)
Del Carmen Avila-Casado, Maria; Perez-torres, Israel; Auron, Ari; Soto, Virgilia; Fortoul, Teresa I.; Herrera-Acosta, Jaime
PUB. DATE
July 2004
SOURCE
Kidney International;Jul2004, Vol. 66 Issue 1, p133
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Proteinuria in rats induced by serum from patients with collapsing glomerulopathy. Background. Primary collapsing glomerulopathy recurs postransplant, raising the possibility of circulating factors implicated in the pathogenesis of the disease. Methods. To determine the presence of circulating factors in collapsing glomerulopathy patients, we tested serum from those patients in an in vivo assay. Eleven groups of rats received serum from collapsing glomerulopathy patients, idiopathic focal segmental glomerulosclerosis (FSGS) or healthy subjects in its native form, isolated IgG, or serum without IgG. The presence of proteinuria and creatinine clearance were determined. Histopathologic analysis included light, immunofluorescence, and electron microscopy. Results. Collapsing glomerulopathy rats developed proteinuria while rats injected with serum from FSGS and healthy subjects did not. Rats injected with serum of collapsing glomerulopathy in its native form developed marked proteinuria (99.2 ± 42 mg/24 hours at day 5, P= 0.0001, compared to the baseline), and decreased in creatinine clearance. Rats receiving isolated IgG or serum without IgG from collapsing glomerulopathy developed mild proteinuria (46.5 ± 8.4 mg/24 hours and 30.9 ± 11 mg/24 hours, respectively, at day 5 ( P= 0.0001). Glomerular tuft retraction and podocyte damage were seen only in collapsing glomerulapthy rats. No abnormalities were found in rats injected with serum from FSGS or healthy subjects. Conclusion. Circulating factors in the serum of collapsing glomerulopathy patients produce podocyte damage, whereas such factors are not present in noncollapsing FSGS. IgG eluates from collapsing glomerulopathy produce proteinuria when injected into the rat. Such factors remain in the circulation when serum of patients is adsorbed into protein A, raising the possibility that there are more than one circulating factor present in patients with collapsing glomerulopathy.
ACCESSION #
13347244

 

Related Articles

  • Insulin Resistance and Vitamin D Deficiency in Patients With Chronic Kidney Disease Stage 2-3. ŠTEFÍKOVÁ, K.; SPUSTOVÁ, V.; KRIVOŠÍKOVÁ, Z.; OKŠA, A.; GAZDÍKOVÁ, K.; FEDELEŠOVÁ, V.; DZÚRIK, R. // Physiological Research;2011, Vol. 60 Issue 1, p149 

    No abstract available.

  • Comparison of phenol-based and alternative RNA isolation methods for gene expression analyses. Jakovljević, Ksenija V.; Spasić, Milena R.; Mališić, Emina J.; Dobričić, Jelena D.; Krivokuć, Ana M.; Janković, Radmila N. // Journal of the Serbian Chemical Society;Jul2010, Vol. 75 Issue 7, p1 

    The widespread use of gene expression analyses has been limited by the lack of a critical evaluation of the methods used to extract nucleic acids from human tissues. For evaluating gene expression patterns in whole blood or leukocytes, the method of RNA isolation needs to be considered as a...

  • EXPRESSIONS OF IL-17, IL-21 AND IL-23 IN THE SERUM OF ALLERGIC RHINITIS PATIENTS. Xuekun Huang; Qintai Yang; Yulian Chen; Peng Li; Gehua Zhang; Yuan Li // Journal of Medical Biochemistry;Dec2011, Vol. 30 Issue 4, p323 

    The present study aimed to investigate the expressions of interleukin-17 (IL-17), IL-21 and IL-23 in the serum of allergic rhinitis (AR) patients and to explore their relationship with special IgE (sFgE) in the serum. AR patients (n=24) and healthy subjects (n=12) were re - cruited and serum...

  • Rodent-associated Bartonella febrile illness, Southwestern United States. Iralu, Jonathan; Ying Bai; Crook, Larry; Tempest, Bruce; Simpson, Gary; McKenzie, Taylor; Koster, Frederick; Bai, Ying // Emerging Infectious Diseases;Jul2006, Vol. 12 Issue 7, p1081 

    Serum specimens from 114 patients hospitalized with a febrile illness were tested with an indirect immunofluorescence assay (IFA) using Bartonella antigens prepared from 6 species of sigmodontine rodents and 3 known human Bartonella pathogens: B. henselae, B. quintana, and B. elizabethae. Acute-...

  • Improved Salt Fractionation of Animal Serums for Immunofluorescence Studies. HEBERT, G. ANN // Journal of Dental Research;Jan1976 Supplement 1, Vol. 55, pA33 

    The article discusses improvements to processes involving the salt fractionation of animal serums in order to prepare for immunofluorescence studies. The tendency for dye to attach to the albumin within serum fractions containing appreciable amounts of albumin upon labeling with fluorescein...

  • Analysis of 15 patients with systemic lupus erythematosus manifesting with negative immunofluorescence anti-nuclear antibodies after treatment. Song, L-J; Ding, F; Liu, H-X; Shu, Q; Yu, X; Li, J; Li, X-F // Lupus;Aug2012, Vol. 21 Issue 9, p919 

    The purpose of this study was to investigate the clinical and laboratorial characteristics of patients with systemic lupus erythematosus (SLE) manifesting with negative immunofluorescence anti-nuclear antibodies (IFANA) after treatment for the better understanding of negative conversion of...

  • Detection of PRL-3 protein in the preoperative serum of patients with colorectal cancer. Pryczynicz, A.; Dymicka-Piekarska, V.; Niewiarowska, K.; Gryko, M.; Niksa, M.; Hawryluk, M.; Kemona, A.; Famulski, W. // Progress in Health Sciences;2013, Vol. 3 Issue 1, p49 

    Purpose: The aim of study was to detect PRL-3 protein in sera of patients with colorectal cancer. Methods: The study group consisted of 22 patients with colorectal carcinoma and 10 healthy controls. The serum concentration of PRL-3 protein was evaluated by the ELISA method. Results: In the study...

  • Serum Immunoglobulins in non-Hodgkin's Lymphoma Patients.  // Collegium Antropologicum;Jun2010, Vol. 34 Issue 2, p407 

    No abstract available.

  • UREMIC TOXICITY.  // Nephrology Dialysis Transplantation;May2014, Vol. 29 Issue suppl_3, piii19 

    No abstract available.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics