TITLE

Neonatal ureteral obstruction alters expression of renal sodium transporters and aquaporin water channels

AUTHOR(S)
Yimin Shi; Chunling Li; Thomsen, Klaus; Jørgensen, Troels Munch; Knepper, Mark A.; Nielsen, Søren; Djurhuus, Jens Christian; Frøki&aezig;r, Jørgen
PUB. DATE
July 2004
SOURCE
Kidney International;Jul2004, Vol. 66 Issue 1, p203
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Neonatal ureteral obstruction alters expression of renal sodium transporters and aquaporin water channels. Background. Congenital urinary tract obstruction is a common cause of renal insufficiency in the neonate and during infancy. Recently, we demonstrated that ureteral obstruction in adult rats is associated with reduction in the abundance of renal aquaporins (AQPs) and renal sodium transporters, which paralleled an impaired urinary concentrating capacity. Methods. In the present study, renal handling of sodium and water, together with the expression of renal aquaporins and major renal sodium transporters, was examined in rats with neonatally induced partial unilateral ureteral obstruction (PUUO) within the first 48 hours of life to clarify the molecular mechanisms involved in the tubular functional defects in response to congenital obstruction. Rats were then followed for 12 or 24 weeks. Results. Neonatal PUUO caused a progressive reduction in single kidney glomerular filtration rate (SKGFR) on the obstructed side to 43% of controls at 12 weeks (115 ± 28 vs. 267 ± 36 μL/min/100g bw, P < 0.05), and 31% of controls at 24 weeks (106 ± 24 vs. 343 ± 41 μL/min/100g bw, P < 0.05). Na-K-ATPase abundance was decreased in the obstructed kidney compared with the nonobstructed kidney at 24 weeks (79 ± 6%, P < 0.05), and the abundance of bumetanide-sensitive Na-K-2Cl cotransporter (BSC-1) located to the medullary thick ascending limb (mTAL) of the obstructed kidney was significantly reduced both at 12 weeks (42 ± 10%, P < 0.05) and 24 weeks (50 ± 10%, P < 0.05). Immunohistochemistry confirmed down-regulation of BSC-1 both at 12 and 24 weeks after onset of obstruction. Consistent with this, sodium excretion from the obstructed kidney was increased at 12 weeks (0.13 ± 0.03 vs. 0.04 ± 0.01 μmol/min/100g bw, P < 0.05), and persisted 24 weeks after onset of PUUO (0.15 ± 0.02 vs. 0.06 ± 0.01 μmol/min/100g bw, P < 0.05). AQP2 abundance in the collecting duct was also reduced both at 12 weeks (68 ± 5%, P < 0.05) and at 24 weeks (69 ± 13%, P < 0.05). Consistent with this, solute-free water reabsorption was decreased in the obstructed kidney at 12 weeks (0.61 ± 0.42 vs. 1.97 ± 0.63 μL/min/100g bw, P < 0.05) and remained decreased after 24 weeks of PUUO (0.42 ± 0.04 vs. 1.56 ± 0.39 μL/min/100g bw, P < 0.05). Conclusion. Major sodium transporters and aquaporins in the obstructed kidney are down-regulated in response to neonatally induced PUUO, which indicates that these transporters may play a crucial role for the persistent reduction in renal handling of sodium and water in response to PUUO.
ACCESSION #
13347238

 

Related Articles

  • Urinary aquaporin-2 excretion during early human development. Zelenina, Marina; Yanhong Li; Glorieux, Isabelle; Arnaud, Catherine; Cristini, Christelle; Decramer, Stéphane; Aperia, Anita; Casper, Charlotte // Pediatric Nephrology;Jul2006, Vol. 21 Issue 7, p947 

    This study was undertaken to assess one of the determinants of kidney concentrating capacity, aquaporin-2 (AQP2), in order to understand the physiopathology of water balance in newborn babies. Urinary AQP2 excretion has been shown to be proportional to AQP2 level in the apical plasma membrane of...

  • Interleukin-10 deficiency aggravates kidney inflammation and fibrosis in the unilateral ureteral obstruction mouse model. Jin, Yuanmeng; Liu, Ruijie; Xie, Jingyuan; Xiong, Huabao; He, John Cijiang; Chen, Nan // Laboratory Investigation (00236837);Jul2013, Vol. 93 Issue 7, p801 

    Interleukin-10 functions as a general immunosuppressive cytokine, which also negatively regulates inflammatory responses through complex mechanisms. Recent studies suggested that IL-10 may also inhibit fibrosis in various diseased models. However, the role of IL-10 in renal fibrosis has not been...

  • Inhibitory effects of fasudil on renal interstitial fibrosis induced by unilateral ureteral obstruction. ITSUKO BABA; YASUHIRO EGI; HIROYUKI UTSUMI; TETSUHIRO KAKIMOTO; KAZUO SUZUKI // Molecular Medicine Reports;2015, Vol. 12 Issue 6, p8010 

    Renal fibrosis is the major cause of chronic kidney disease, and the Rho/Rho-associated coiled-coil kinase (ROCK) signaling cascade is involved in the renal fibrotic processes. Several studies have reported that ROCK inhibitors attenuate renal fibrosis. However, the mechanism of this process...

  • Role of angiotensin II in the expression and regulation of transforming growth factor-β in obstructive nephropathy. Pimentel Jr., J. Luis; Sundell, Cynthia L.; Susheng Wang; Kopp, Jeffrey B.; Montero, Ángel; Martínez-Maldonado, Manuel // Kidney International;Oct1995, Vol. 48 Issue 4, p1233 

    Unilateral ureteral obstruction (UUO) leads to fibrosis of the obstructed kidney. We tested the hypothesis that interstitial fibrosis in UUO results, at least in part, from enhanced expression of transforming growth factor-β (TGF-β) which in turn is regulated by local angiotensin II (Ang...

  • A neonate with acute renal failure: question. Bellini, Carlo; Campone, Francesco // Pediatric Nephrology;Jan2008, Vol. 23 Issue 1, p49 

    A quiz concerning the acute renal failure of a new born infant is presented.

  • Altered expression of immune modulator and structural genes in neonatal unilateral ureteral obstruction. Silverstein, Douglas M.; Travis, Brett R.; Thornhill, Barbara A.; Schurr, Jill S.; Kolls, Jay K.; Leung, Jocelyn C.; Chevalier, Robert L. // Kidney International;Jul2003, Vol. 64 Issue 1, p25 

    Altered expression of immune modulator and structural genes in neonatal unilateral ureteral obstruction. Background. Congenital obstructive nephropathy is a condition characterized by hydronephrosis, tubular dilatation, apoptosis, and atrophy, as well as interstitial cellular infiltration and...

  • Case of single system obstructive megaureter with obstructive ureterocele, segmental atropic ureter and dysgenetic kidney. Narasimhaiah, K.; Keshavamurthy, R.; Nagaraj, N. H.; Kamath, A. J.; Chandrashekar, C. R.; Rathkal, C. S.; Venkatesh, G. K. // Indian Journal of Urology;Supplement1, Vol. 24, pS35 

    Master Chethan, aged 5 yrs, presented to our department with hematuria. On examination: Bladder was distended, phimosis present Investigations: Hb:%: 12.5 gm%, RBS: 69 mg%, Blood urea: 30 mg%, S. creatinine: 0.7 mg% Ultrasound abdomen: Absent right kidney and cystic mass in RIF IVU: Right kidney...

  • Renal cilia display length alterations following tubular injury and are present early in epithelial repair. Elizabeth Verghese; Raphael Weidenfeld; John F. Bertram; Sharon D. Ricardo; James A. Deane // Nephrology Dialysis Transplantation;Mar2008, Vol. 23 Issue 3, p834 

    Background. Renal cilia are flow sensors that are required for the maintenance of normal kidney architecture. Defects in this organelle are frequently associated with polycystic kidney disease, but the role of renal cilia during acute tubular injury has not been investigated. Methods. We have...

  • DNA oligonucleotide microarray technology identifies fisp-12 among other potential fibrogenic genes following murine unilateral ureteral obstruction (UUO): modulation during epithelial-mesenchymal transition. Higgins, Debra F.; Lappin, David W.P.; Kieran, Niamh E.; Anders, Hans J.; Watson, Ronald W.G.; Strutz, Frank; Schlondorff, Detlef; Haase, Volker H.; Fitzpatrick, John M.; Godson, Catherine; Brady, Hugh R. // Kidney International;Dec2003, Vol. 64 Issue 6, p2079 

    DNA oligonucleotide microarray technology identifies fisp-12 among other potential fibrogenic genes following murine unilateral ureteral obstruction (UUO): Modulation during epithelial-mesenchymal transition. Background. Tubulointerstitial inflammation and fibrosis are pathologic hallmarks of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics