TITLE

Aldosterone enhances renal calcium reabsorption by two types of channels

AUTHOR(S)
Leclerc, Marie; Brunette, Michele G.; Couchourel, Denis
PUB. DATE
July 2004
SOURCE
Kidney International;Jul2004, Vol. 66 Issue 1, p242
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Aldosterone enhances renal calcium reabsorption by two types of channels. Background. Aldosterone has been known for many years to increase sodium (Na+) reabsorption by the distal nephron. The present in vitro experiments investigated the effect of the hormone on calcium (Ca2+) transport by the luminal membrane of the rabbit nephron, independent of any systemic influence. Methods. Proximal and distal tubules were incubated with either aldosterone or the carrier. The luminal membranes of these tubules were purified, vesiculated, and 45Ca uptake by these vesicles was subsequently measured. Results. Treatment of the distal tubules with 10−8 mol/L aldosterone enhanced both 0.1 and 0.5 mmol/L Ca2+ transport. The hormone action was abolished by tyrosine kinase inhibitors. The presence of Na+ in the medium decreased both Ca2+ uptake and the effect of aldosterone. This hormone action was already significant after a 5-minute incubation, with a half-maximal efficient concentration of approximately 10−10 mol/L. Ca2+ transport by the distal membranes presents a dual kinetics. Aldosterone enhanced the Vmax values of both components of these kinetics. Mibefradil abolished the action of aldosterone on 0.5 mmol/L but not on 0.1 mmol/L Ca2+ uptake, suggesting that the targeted low affinity channel belongs to the T-type, whereas diltiazem prevented the hormone action exclusively at the low Ca2+ concentration (0.1 mmol/L), indicating an effect on a high affinity L-type channel. Conclusion. Aldosterone increases Ca2+ transport by the distal luminal membranes through L- and T-type Ca2+ channels, and this action requires tyrosine kinase activity.
ACCESSION #
13347234

 

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