TITLE

Increased mast cells and neutrophils in submucosal mucous glands and mucus plugging in patients with asthma

AUTHOR(S)
Carroll, N. G.; Mutavdzic, S.; James, A. L.
PUB. DATE
August 2002
SOURCE
Thorax;Aug2002, Vol. 57 Issue 8, p677
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Mucus plugging of the airways is invariably seen in cases of fatal asthma, mucus production is associated with asthma attacks, and area of submucosal glands is increased in asthma. Mediators secreted from mast cells and neutrophils can stimulate mucous gland secretion. A study was undertaken to count the mast cells and neutrophils in submucosal glands and to relate cell numbers to the presence of mucus in the airway lumen. Methods: Cartilaginous airways obtained at necropsy from cases of fatal asthma (n = 8), non-fatal asthma (n = 8) were examined. Contiguous transverse sections were stained for mast cell tryptase and neutrophil elastase, and with Periodic Acid Schiff solution to identify mucus. Mucous gland area, lumen area, and the percentage of the relaxed lumen area occupied by mucus (mucus occupying ratio, MOR) were measured. Mast cell (intact and degranulated) and neutrophils per area of submucosal gland were calculated. Results: Compared with controls, the cases of fatal asthma had increased mucous gland area, MOR, percentage of degranulated mast cells, and numbers of neutrophils in the submucosal glands (p < 0.05). In cases of non-fatal asthma the MOR and the numbers of mast cells and neutrophils in the submucosal glands were increased (p < 0.05). When all cases were pooled together, the MOR correlated with the total number of mast cells (r = 0.55, p= 0.005) and with the number of degranulated mast cells in the submucosal glands (r = 0.51, p = 0.013), but not with the number of neutrophils (r = 0.21, p = 0.121). Conclusion: These results show that mucous gland area, MOR and mucous gland inflammation are increased in asthma and that degranulation of mast cell may contribute to secretion of mucus into the lumen in cases of fatal asthma.
ACCESSION #
13221425

 

Related Articles

  • Patients With Bone Marrow Failure Demonstrate Decreased Cutaneous Reactivity to Human C5a. Yancey, Kim B.; Bielory, Leonard; Wright, Ralph; Young, Neal; Frank, Michael M.; Lawley, Thomas J. // Journal of Investigative Dermatology;Apr87, Vol. 88 Issue 4, p388 

    In vivo studies have shown that human C5a, a potent complement-derived anaphylatoxin and chemoattractant, produces immediate inflammatory reactions following intradermal injection in human skin. At concentrations within its potential physiologic range, intradermal injection of C5a elicits...

  • Mast cell tryptase and asthma. Zhang, M-Q.; Timmerman, H. // Mediators of Inflammation;Dec97, Vol. 6 Issue 5/6, p311 

    Recent physiological and pharmacological studies have indicated the potential importance of tryptase, the major protein component in mast cells, in inflammatory diseases (especially asthma). Being released at inflammatory sites after the activation of mast cells, tryptase is capable of causing...

  • Mast cell migration to Th2 stimulated airway smooth muscle from asthmatics. Sutcliffe, A.; Kaur, D.; Page, S.; Woodman, L.; Armour, C. L.; Baraket, M.; Bradding, P.; Hughes, J. M.; Brighiling, C. E. // Thorax;Aug2006, Vol. 61 Issue 8, p657 

    Background: Mast cell microlocalisation within the airway smooth muscle (ASM) bundle is an important determinant of the asthmatic phenotype. We hypothesised that mast cells migrate towards ASM in response to ASM derived chemokines. Methods: Primary ASM cultures from subjects with and without...

  • Correlation of Cyclooxygenase 2 Expression and Inflammatory Cells Infiltration in Colorectal Cancer. Naghshvar, F.; Torabizadeh, Zh.; Emadian, O.; Enami, K.; Ghahremani, M. // Pakistan Journal of Biological Sciences;2009, Vol. 12 Issue 1, p98 

    This study want to determine correlation of cyclooxygenans 2 (Cox-2) expression and inflammatory reaction in colorectal carcinoma. Archival H and E slides of 150 patients with primary colorectal carcinoma were reviewed to confirm pathological feature and to select suitable tissue blocks for...

  • Mast cell-derived mediators promote murine neutrophil effector functions. Doener, Fatma; Michel, Anastasija; Reuter, Sebastian; Friedrich, Pamela; Böhm, Livia; Relle, Manfred; Codarri, Laura; Tenzer, Stefan; Klein, Matthias; Bopp, Tobias; Schmitt, Edgar; Schild, Hansjörg; Radsak, Markus Philipp; Taube, Christian; Stassen, Michael; Becker, Marc // International Immunology;Oct2013, Vol. 25 Issue 10, p553 

    Mast cells are able to trigger life-saving immune responses in murine models for acute inflammation. In such settings, several lines of evidence indicate that the rapid and protective recruitment of neutrophils initiated by the release of mast cell-derived pro-inflammatory mediators is a key...

  • Mast Cell-Mediated Inhibition of Abdominal Neutrophil Inflammation by a PEGylated TLR7 Ligand. Hayashi, Tomoko; Shiyin Yao; Crain, Brian; Chan, Michael; Cottam, Howard B.; Lao, Fitzgerald; Carson, Dennis A.; Corr, Maripat // Mediators of Inflammation;2012, Vol. 2012, p1 

    Although the mechanisms for sustained chemokine gradients and recurring cell infiltration in sterile peritonitis have not been elucidated, toll-like receptors (TLRs) have been implicated. To abate the deleterious recruitment of neutrophils in sterile inflammation, we repeatedly administered a...

  • Neutrophilic infiltration within the airway smooth muscle in patients with COPD. Baraldo, S.; Turato, G.; Badin, C.; Bazzan, E.; Beghé, B.; Zuin, R.; Calabrese, F.; Casoni, G.; Maestrelli, P.; Papi, A.; Fabbri, L. M.; Saetta, M. // Thorax;Apr2004, Vol. 59 Issue 4, p308 

    Background: COPD is an inflammatory disorder characterised by chronic airflow limitation, but the extent to which airway inflammation is related to functional abnormalities is still uncertain. The interaction between inflammatory cells and airway smooth muscle may have a crucial role. Methods:...

  • CALCIUM IONOPHORE A23187 AND COMPOUND 48/80 INDUCE PGD2 AND TRYPTASE IN HUMAN CORD BLOOD-DERIVED MAST CELLS: LACK OF EFFECT OF IL-18. MACCAURO, G.; TRIPODI, D.; SAGGINI, A.; CONTI, F.; CIANCHETTI, E.; ANGELUCCI, D.; ROSATI, M.; TONIATO, E.; FULCHERI, M.; TETÈ, S.; SALINI, V.; CARAFFA, A.; ANTINOLFI, P.; FRYDAS, S.; CONTI, P.; THEOHARIDES, T. C. // European Journal of Inflammation (BIOLIFE, s.a.s.);Jan-Apr2012, Vol. 10 Issue 1, p33 

    Immunological and biochemical reactions associated with inflammation are elicited in response to a physical or immunological challenge. Early in inflammation there is mobilization and infiltration of neutrophils, mast cells and macrophages to the site of inflammation. These cells release...

  • Mechanisms of Antihistamines and Mast Cell Stabilizers in Ocular Allergic Inflammation. Cook, E.B.; Stahl, J.L.; Barney, N.P.; Graziano, F.M. // Current Drug Targets - Inflammation & Allergy;Jun2002, Vol. 1 Issue 2, p167 

    Mast cells play a central role in allergic reactions and inflammation. Successful anti-allergic therapies have typically targeted mast cell mediators, particularly histamine. Antihistaminic compounds interact with the various histamine receptors found on many cells, whereas other compounds such...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics