April 2004
Gut;Apr2004 Supplement 3, Vol. 53, pA98
Academic Journal
In ulcerative colitis, upregulation of inducible nitric oxide synthase occurs in colonic epithelial cells along with increased production of nitric oxide (NO). The article examines the potential toxicity of NO to colonic epithelial cells. For the study HT29 cells were cultured with NO donor DETA NONOate. Supraphysiological concentrations of NO results in both necrosis and apoptosis of colonic epithelial cells. The evidence also suggested that neither the carrier molecule nor stable by products of NO degradation are important.


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