TITLE

OVARIAN AND COLON CELL LINES CAN BE DIFFERENTIATED USING SELDI-MS PROTEIN PROFILING

AUTHOR(S)
Lennon, A. M.; Sturat, D.; Tan, A. C.; Sheahan, K. S.; O'Donoghue, D. P.; Brown, B.
PUB. DATE
April 2004
SOURCE
Gut;Apr2004 Supplement 3, Vol. 53, pA67
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Surface enhanced laser desorption ionisation mass spectrometry (SELDI-MS) is a technique which has been used to study proteomic patterns. The article presents a study which aims to determine the sensitivity of SELDI-MS in differentiating two different cell lines. The study demonstrates the potential of SELDI-MS to differentiate two different cell lines with a high level of sensitivity and specificity. 198 protein peaks were generated from 13 cell line extracts. Ciphergen Biomarker Wizard was used to identify significant peaks and an in-house decision analysis tree was used to differentiate the two sample types.
ACCESSION #
13219077

 

Related Articles

  • SELDI-MS PROTEIN PROFILING CAN DIFFERENTIATE TUMOUR FROM NORMAL COLORECTAL TISSUE. Lennon, A. M.; Stuart, D.; Tan, A. C.; Fox, E.; Sheahan, K. S.; Hyland, J. M.; O'Donoghue, D. P.; Brown, B. // Gut;Apr2004 Supplement 3, Vol. 53, pA68 

    The article presents a study that uses Surface enhanced laser desorption ionisation mass spectrometry (SELDI-MS) to examine the feasibility of differentiating tumor from normal colorectal tissue. SELDI-MS is a technique which has been used to study proteomic patterns in cell lines, tumor...

  • A quantitative analysis software tool for mass spectrometry—based proteomics. Sung Kyu Park; Venable, John D.; Tao Xu; Yates, III, John R. // Nature Methods;Apr2008, Vol. 5 Issue 4, p319 

    We describe Census, a quantitative software tool compatible with many labeling strategies as well as with label-free analyses, single-stage mass spectrometry (MS1) and tandem mass spectrometry (MS/MS) scans, and high- and low-resolution mass spectrometry data. Census uses robust algorithms to...

  • A dynamic wavelet-based algorithm for pre-processing tandem mass spectrometry data. Penghao Wang; Pengyi Yang; Arthur, Jonathan; Yang, Jean Yee Hwa // Bioinformatics;Sep2010, Vol. 26 Issue 18, p2242 

    Motivation: Mass spectrometry (MS)-based proteomics is one of the most commonly used research techniques for identifying and characterizing proteins in biological and medical research. The identification of a protein is the critical first step in elucidating its biological function. Successful...

  • Protein Identification in Sub Proteome Fractions of Breast Cancer Cells by OFFGEL-IEF and iTRAQ Labeling. Chandramouli, K. H.; Agrawal, Pushpa; Thimmaiah, K. N. // Current Proteomics;Apr2009, Vol. 6 Issue 1, p43 

    To better understand the molecular mechanisms underlying breast cancer metastasis and search for potential markers, we applied OFFGEL-IEF, iTRAQ labeling and mass spectrometry (LC- MS/MS) analysis to identify proteins in MDA-MB-435 breast cancer cell line. Protein expression profiling might...

  • Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines. Qian-chen Guo; Jing-nan Shen; Song Jin; Jin Wang; Gang Huang; Long-juan Zhang; Jun-qiang Yin; Chang-ye Zou; Ming-tao Li // Acta Pharmacologica Sinica;Jun2007, Vol. 28 Issue 6, p850 

    Aim: Comparative proteomics provide a powerful approach in screening for alterations in protein levels and post-translational modifications that are associated with tumors. In the present study, we aimed to identify candidate biomarkers to distinguish osteosarcoma (OS) cells from normal...

  • Super-SILAC mix for quantitative proteomics of human tumor tissue. Geiger, Tamar; Cox, Juergen; Ostasiewicz, Pawel; Wisniewski, Jacek R.; Mann, Matthias // Nature Methods;May2010, Vol. 7 Issue 5, p383 

    We describe a method to accurately quantify human tumor proteomes by combining a mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell lines with human carcinoma tissue. This generated hundreds of thousands of isotopically labeled peptides in appropriate...

  • Mass Spectrometry-Based Approaches Toward Absolute Quantitative Proteomics. Kito, Keiji; Ito, Takashi // Current Genomics;Jul2008, Vol. 9 Issue 4, p263 

    Mass spectrometry has served as a major tool for the discipline of proteomics to catalogue proteins in an unprecedented scale. With chemical and metabolic techniques for stable isotope labeling developed over the past decade, it is now routinely used as a method for relative quantification to...

  • Approaches to dimensionality reduction in proteomic biomarker studies. Hilario, Melanie; Kalousis, Alexandros // Briefings in Bioinformatics;Mar2008, Vol. 9 Issue 2, p102 

    Mass-spectra based proteomic profiles have received widespread attention as potential tools for biomarker discovery and early disease diagnosis. A major data-analytical problem involved is the extremely high dimensionality (i.e. number of features or variables) of proteomic data, in particular...

  • Algorithms and tools for analysis and management of mass spectrometry data. Veltri, Pierangelo // Briefings in Bioinformatics;Mar2008, Vol. 9 Issue 2, p144 

    Mass spectrometry (MS) is a technique that is used for biological studies. It consists in associating a spectrum to a biological sample. A spectrum consists of couples of values (intensity, m/z), where intensity measures the abundance of biomolecules (as proteins) with a mass-to-charge ratio...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics