Otto, W. R.; McKinnell, I.; Lee, C. Y.; Frith, D.; Hanrahan, S.; Blin, N.; Kayademir, T.; Poulsorn, R.; Jeffery, R.; Hunt, T.; Wright, N. A.; Oien, K. A.; McGregor, F.; Patel, K.
April 2004
Gut;Apr2004 Supplement 3, Vol. 53, pA15
Academic Journal
Receptors for members of the trefoil factor family (TFF) peptides remain elusive. In experiments to try and find such a molecule, authors identified a novel murine TFF2-binding protein, which they have named Blottin. Authors created a fusion protein of mouse TFF2 with secretory embryonic alkaline phosphates (AP), expressed it in mammalian 293 cells, and probed tissue sections for binding activity using culture supernatant, visualising the AP activity directly on the sections. Substantial binding to gastric epithelium was seen, so this tissue was used in 2-D protein analytical SDS-PAGE, with western blots probed using the same ligand, and a single major protein of interest was identified by mass spectrometry. The protein was previously unknown as a translated product, and has only mammalian homologues.


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