TITLE

COELIAC DISEASE: T CELL IMMUNOGENICTY AND IN VIVO TOXICITY OF THE HMW GLUTENINS

AUTHOR(S)
Dewar, D. H.; Amato, M.; Wieser, H.; Cilitira, P. J.
PUB. DATE
April 2004
SOURCE
Gut;Apr2004 Supplement 3, Vol. 53, pA6
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Wheat gluten comprises gliadins, which exacerbate coeliac disease (CD), and glutenin subunits, whose role in CD remains obscure. Authors wished to study T cell immunogenicity and in vivo toxicity of high molecular weight glutenin subunits (HMW-GS). The results demonstrate the presence of HMW-GS specific T cells in CD small intestine, and the in vivo toxicity of this fraction. The demonstration of ILl 5 staining at 2h indicates that HMW-GS might be toxic via early mediators, prior to the activation of antigen specific CD4+T cells. The fraction consists of four different subunits. The data do not indicate the relative toxicity of single subunits, whose preparative separation by chemical means is difficult. Use of pure recombinant HMW-GS in similar experiments will elucidate the suitability of these proteins for inclusion in foods for consumption by patients with CD.
ACCESSION #
13218836

 

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