TITLE

In vivo targeting of intestinal and extraintestinal transglutaminase 2 by coeliac autoantibodies

AUTHOR(S)
Korponay-Szabó, I. R.; Halttunen, I.; Szalai, Z.; Laurila, K.; Király, R.; Kovács, J. B.; Fésüs, L.; Möki, M.
PUB. DATE
May 2004
SOURCE
Gut;May2004, Vol. 53 Issue 5, p641
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: IgA class serum autoantibodies against type 2 (tissue) transglutaminase (TG2) bind to both intestinal and extraintestinal normal tissue sections in vitro, eliciting endomysial, reticulin, and jelunal antibody reactions. It is not known whether similar binding also occurs in coeliac patients in vivo, and may thereby contribute to disease manifestations. Aims: To investigate intestinal and extraintestinal coeliac tissues for the presence of in vivo bound TG2 specific IgA and its relation to small intestinal mucosal atrophy. Patients: We investi9ated lelunal samples with normal villous morphology from 10 patients with developing coeliac disease who subsequently progressed to a flat lesion, from 11 patients with dermatitis herpetiformis, and from 12 non-coeliac controls. Six extrajelunal biopsy samples (liver, lymph node, muscle, appendix), obtained based on independent clinical indications from patients with active coeliac disease, were also studied. Methods: Double colour immunofluorescent studies For in situ IgA, TG2, and laminin were performed. IgA was eluted from tissue sections and tested for TG2 specificity by enzyme linked immunosorbent assay and indirect immunofluorescence. Results: IgA (in one IgA deficient case IgG) deposition on extracellularly located TG2 was detected in lelunal and extra jelunal specimens of all coeliac patients, and also in seven of 11 dermatitis herpetiformis patients, of whom two had no circulating endomysial antibodies. IgA eluted from extraintestinal coeliac tissues was targeted against TG2. Conclusions: Coeliac IgA targets jejunal TG2 early in disease development even when endomysial antibodies are not present in the circulation. Extraintestinal target sites of coeliac lgA further indicate that humoral immunity may have a pathogenetic role.
ACCESSION #
13148296

 

Related Articles

  • What blood tests help diagnose celiac disease? Reddick, Bonzo K.; Crowell, Karen // Journal of Family Practice;Dec2006, Vol. 55 Issue 12, p1088 

    The article discusses the kind of blood tests which help diagnose celiac disease. The study has revealed that the histological confirmation of infiltrative lesions thru small bowel biopsy was the gold standard for diagnosing celiac disease. It has noted four serum antibody assays which may serve...

  • Transglutaminase-Catalyzed Reactions Responsible for the Pathogenesis ofCeliac Disease and Neurodegenerative Diseases: From Basic Biochemistry to Clinic. Martin, A.; Romito, G.; Pepe, I.; De Vivo, G.; Merola, M. R.; Limatola, A.; Gentile, V. // Current Medicinal Chemistry;2006, Vol. 13 Issue 16, p1895 

    Transglutaminases (TGases) are enzymes which catalyze the cross linking of a glutaminyl residue of a protein/peptide substrate to a lysyl residue of a protein/peptide co-substrate with the formation of an N-gamma-(epsilon- L-glutamyl)-L-lysine [GGEL] cross link (isopeptidic bond) and the...

  • Secretion of celiac disease autoantibodies after in vitro gliadin challenge is dependent on small-bowel mucosal transglutaminase 2-specific IgA deposits. Stenman, Satumarja M.; Lindfors, Katri; Korponay-Szabo, Ilma R.; Lohi, Olli; Saavalainen, Päivi; Partanen, Jukka; Haimila, Katri; Wieser, Herbert; Mäki, Markku; Kaukinen, Katri // BMC Immunology;2008, Vol. 9, Special section p1 

    Background: In celiac disease gluten, the disease-inducing toxic component in wheat, induces the secretion of autoantibodies which are targeted against transglutaminase 2 (TG2). These autoantibodies are produced in the smallintestinal mucosa, where they can be found deposited extracellularly...

  • Suboptimal Performance of IgG Anti-tissue Transglutaminase in the Diagnosis of Celiac Disease in a Tropical Country. Dutta, Amit Kumar; Chacko, Ashok; Avinash, Balekuduru // Digestive Diseases & Sciences;Mar2010, Vol. 55 Issue 3, p698 

    Serological tests using human IgA-anti-tTG have been reported to have high sensitivity and specificity in diagnosis of celiac disease. There is a paucity of data on the use of human IgG-anti-tTG in diagnosis of celiac disease. Ninety-two patients with clinical suspicion of celiac disease who...

  • An ELIME assay for the rapid diagnosis of coeliac disease. Adornetto, Gianluca; Volpe, Giulia; Stefano, Alessia; Martini, Sonia; Gallucci, Giuseppina; Manzoni, Angelo; Bernardini, Sergio; Mascini, Marco; Moscone, Danila // Analytical & Bioanalytical Chemistry;Aug2012, Vol. 403 Issue 4, p1191 

    Coeliac disease (CD) is a gluten-induced autoimmune enteropathy found in genetically susceptible subjects. Because of the high number of undetected cases, rapid and cheaper screening methods are needed. Currently, the CD diagnosis involves the detection of anti-transglutaminase IgA antibodies...

  • Anti-Tissue Transglutaminase Antibodies in Patients with Abnormal Liver Tests: Is It Always Coeliac Disease? Iacono, Oreste Lo; Petta, Salvatore; Venezia, Giovanna; Di Marco, Vito; Tarantino, Giuseppe; Barbaria, Francesco; Mineo, Claudia; De Lisi, Stefania; Almasio, Piero Luigi; Craxì, Antonio // American Journal of Gastroenterology;Nov2005, Vol. 100 Issue 11, p2472 

    BACKGROUND: Coeliac disease (CD) is found in 5–10% of patients with chronically abnormal liver tests and no obvious cause of liver disease. In this population the efficacy of screening for CD by anti-tissue transglutaminase (anti-tTG) may be impaired by the high rate of positive anti-tTG...

  • Celiac Disease. Presutti, R. John; Cangemi, John R.; Cassidy, Harvey D.; Hill, David A. // American Family Physician;12/15/2007, Vol. 76 Issue 12, p1795 

    As many as one in every 100 to 200 persons in the United States has celiac disease, a condition resulting from an inappropriate immune response to the dietary protein gluten. The manifestations of celiac disease range from no symptoms to overt malabsorption with involvement of multiple organ...

  • Screening for Celiac Disease in a North American Population: Sequential Serology and Gastrointestinal Symptoms. Katz, Kent D.; Rashtak, Shahrooz; Lahr, Brian D.; Melton, L. Joseph; Krause, Patricia K.; Maggi, Kristine; Talley, Nicholas J.; Murray, Joseph A. // American Journal of Gastroenterology;Jul2011, Vol. 106 Issue 7, p1333 

    OBJECTIVES:The prevalence of diagnosed celiac disease is <1 in 2,000 in the United States, but screening studies undertaken in European and other populations have revealed a much higher prevalence. The objective of this study was to determine the prevalence of celiac disease and the utility of...

  • Atypical Presentation is Dominant and Typical for Coeliac Disease. Nejad, Mohammad Rostami; Rostami, Kamran; Pourhoseingholi, Mohamad Amin; Mojarad, Ehsan Nazemalhosseini; Habibi, Manijeh; Dabiri, Hossein; Zali, Mohammad Reza // Journal of Gastrointestinal & Liver Diseases;Sep2009, Vol. 18 Issue 3, p285 

    Objectives: Atypical presentation is the most prevalent form of coeliac disease (CD) and mostly clinically indistinguishable from other gastrointestinal (GI) disorders. The first objective of this study was to determine the prevalence of CD in patients with GI symptoms and the second objective...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics