TITLE

Atherogenic Effect of Interleukin-2 and Antiatherogenic Effect of Interleukin-2 Antibody in Apo-E-Deficient Mice

AUTHOR(S)
Upadhya, Subramanya; Mooteri, Sahantalaxmi; Peckham, Norman; Pai, Ramdas G.
PUB. DATE
May 2004
SOURCE
Angiology;May/Jun2004, Vol. 55 Issue 3, p289
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Growing evidence suggests that atherosclerosis is an immune-mediated inflammatory process and that cytokines participate as mediators in this process. Of the cytokines, interleukins, which are released from both immune and nonimmune cells of vascular wall, are found to have multiple effects. Interleukin-2 (IL-2), a cytokine produced by activated T-lymphocytes, has been found to further activate the T cells and may potentially enhance atherogenesis. Apo-Edeficient mice fed with atherogenic diet were injected intraperitoneally twice a week with placebo, IL-2, or anti-IL-2 antibody for a period of 6 weeks. Group 1 (n = 6) was injected with bovine serum albumin (BSA) in phosphate-buffered saline (PBS) and served as controls. Group 2 (n=6) was injected with 2 × 104 units of recombinant murine IL-2 (rmIL-2) per dose reconstituted with BSA in PBS. Group 3 (n=6) was injected with 5 µg of anti-IL-2 per dose reconstituted with BSA in PBS. Aortic sections were analyzed and atherosclerotic burden was quantified. Compared to controls, injection of IL-2 increased measures of atherosclerosis such as the average lesion score (10.7 ± 0.5 vs 9.3 ± 1.1, p=0.04) and the lesion size as a fraction of aortic area (0.51 ± 0.03 vs 0.41 ± 0.05, p=0.01). Injection of anti-IL-2 had a profound antiatherogenic effect. It significantly reduced the average number of lesions per cross section (2.6 ± 0.6 vs 4.3 ± 0.6, p = 0.03), the average lesion score (4.6 ± 1.9 vs 9.3 ± 1.1, p = 0.02), the lesion area/circumference (0.35 ± 0.08 vs 0.62 ± 0.10, p=0.007), and the lesion size/aortic area (0.23 ± 0.07 vs 0.41 ±0.05, p=0.03). These results indicate that IL-2 is an atherogenic cytokine in apo-Edeficient mice and anti-IL-2 is protective against atherosclerosis. This may have important clinical implications in modifying the atherosclerotic process.
ACCESSION #
13143112

 

Related Articles

  • IL-1 and atherosclerosis: a murine twist to an evolving human story. Rader, Daniel J. // Journal of Clinical Investigation;Jan2012, Vol. 122 Issue 1, p27 

    Inflammation is a critical component of atherosclerosis. IL-1 is a classic proinflammatory cytokine that has been linked to atherosclerosis. A clinical trial has been launched in which an antibody specific for IL-1β is being studied for its effects on cardiovascular events in patients with...

  • Effect of Interleukin-2 Level and Genetic Variants on Coronary Artery Disease. Ding, Ru; Gao, Wenwu; Ostrodci, David; He, Zhiqing; Song, Yuanlin; Ma, Lan; Liang, Chun; Wu, Zonggui // Inflammation;Dec2013, Vol. 36 Issue 6, p1225 

    Inflammation plays important roles in the development of atherosclerosis and coronary artery disease (CAD). Interleukin-2 (IL-2) is a proinflammatory cytokine and induces proliferation of T cells. The aim of the study was to understand the effect of IL-2 on the development of CAD from genetic...

  • The Role of T-Helper Cells in Atherosclerosis. Lu, X.; Lu, D.; Narayan, U.; Kakkar, V. V. // Cardiovascular & Hematological Agents in Medicinal Chemistry;2011, Vol. 9 Issue 2, p25 

    No abstract available.

  • NEW INSIGHTS INTO CHRONIC INFLAMMATION AND ATHEROSCLEROSIS. Silaghi, Cristian; Cristea, Victor // Romanian Journal of Rheumatology;2007, Vol. 16 Issue 4, p213 

    The central role of chronic inflammation in atherogenesis is now well documented and there is increasing evidence of immune and inflammatory pathways involved in the pathogenesis of atherosclerosis. The review summarizes the involvement of cytokines in all stages of plaque development, the...

  • Production of IFN-γ by CD4+ T cells in response to malaria antigens is IL-2 dependent. Kimura, Daisuke; Miyakoda, Mana; Honma, Kiri; Shibata, Yoshisada; Yuda, Masao; Chinzei, Yasuo; Yui, Katsuyuki // International Immunology;Dec2010, Vol. 22 Issue 12, p941 

    T-cell immune responses are critical for protection of the host and for disease pathogenesis during infection with Plasmodium species. We examined the regulation of CD4+ T-cell cytokine responses during infection with Plasmodium berghei ANKA (PbA). CD4+ T cells from PbA-infected mice produced...

  • Circulating 1L-2 and 1L-10 in Chronic Active Hepatitis C with Respect to the Response to 1FN Treatment. Bozkaya, H.; Bozdayi, A. M.; Aslan, N.; Türkay, C.; Sarioglu, M.; Çetinkaya, H.; Akdogan, M.; Cinar, K.; Erden, E.; Köse, K.; Sentürk, H.; Akkiz, H.; Karayalcin, S.; Yurdaydin, C.; Uzunalimoglu, Ö. // Infection;Oct2000, Vol. 28 Issue 5, p309 

    Background: The importance of circulating immunoregulatory cytokines in response to IFN treatment and the change of in vivo production of these cytokines during interferon (IFN) treatment are not well known. We aimed to determine whether pretreatment serum levels of IL-2 and IL-10 are predictive...

  • Up-regulation of interleukin-2 mRNA in children with idiopathic nephrotic syndrome. Shimoyama, Hironobu; Nakajima, Mitsuru; Naka, Hiroyuki; Maruhashi, Yoshiyuki; Akazawa, Hideki; Ueda, Taku; Nishiguchi, Masayuki; Yamoto, Yoko; Kamitsuji, Hidekazu; Yoshioka, Akira // Pediatric Nephrology;Oct2004, Vol. 19 Issue 10, p1115 

    The current hypothesis for the pathogenesis of childhood idiopathic nephrotic syndrome (INS) favors the involvement of a T cell-mediated immune response. Various cytokines derived from T cells may play a critical role in INS. Previous studies have measured serum or urine cytokine levels and...

  • Circulating cytokine profile in anti-neutrophilic cytoplasmatic autoantibody-associated vasculitis: prediction of outcome? Ohlsson, Sophie; Wieslander, Jörgen; Segelmark, Mårten // Mediators of Inflammation;Aug2004, Vol. 13 Issue 4, p275 

    A ims The anti-neutrophilic cytoplasmatic autoantibody-associated vasculitides (AASV) are diseases of relapsing-remitting inflammation. Here we explore the cytokine profile in different phases of disease, looking for pathogenic clues of possible prognostic value. Results Interleukin (IL)-6, IL-8...

  • The Adenosine A2b Receptor: Its Role in Inflammation. Ham, J.; Rees, D. A. // Endocrine, Metabolic & Immune Disorders - Drug Targets;Dec2008, Vol. 8 Issue 4, p244 

    Investigations into the role of the adenosine A2b receptor have been enigmatic due to the lack of good selective high affinity agonists and antagonists. Over the last few years several new antagonist compounds, based either on a xanthine or pyrrolpyrimidine (polyheterocyclic) structure have been...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics