TITLE

Sputum induction as a research tool for sampling the airways of subjects with cystic fibrosis

AUTHOR(S)
Henig, N. R.; Tonelli, M. R.; Pier, M. V.; Burns, J. L.; Aitken, M. L.
PUB. DATE
April 2001
SOURCE
Thorax;Apr2001, Vol. 56 Issue 4, p306
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background--Sputum induction (SI) has proved to be a reliable non-invasive tool for sampling inflammatory airway contents in asthma, with distinct advantages over collection of expectorated sputum (ES) and bronchoalveolar lavage (BAL). A study was undertaken to evaluate the safety of SI and to assess if it might be an equally valuable outcome tool in patients with cystic fibrosis (CF). Methods--The safety of the procedure was examined and sample volume, cell counts, cytokine concentrations, and bacterial culture results obtained by SI, spontaneous ES, and fibreoptic bronchoscopy were compared in 10 adults with CF. Results-SI was well tolerated and was preferred to HAL by all subjects. The mean (SE) sample volume obtained by SI was significantly greater than ES (6.74 (1.46) ml v 1.85 (0.33) nil, p = 0.005). There was no significant difference in the number of cells per ml of sample collected. There was a difference in the mean (SD) percentage of non-epithelial, non squamous cells collected (67 (28)%, 86 (21)%, and 99 (1)% for ES, SI, and HAL, respectively). These percentage counts were different between ES and both SI and HAL (p=0.03 and p=0.006, respectively). Cell differential counts (excluding squamous cells) from all collection methods were similar (mean (SD) 84 (9)%, 87 (7)%, and 88 (11)% polymorphonuclear cells for ES, SI, and BAL, respectively). The concentrations of interleukin (IL)-8 and tumour necrosis factor (TNF) -a were the same in all three samples when corrected for dilution using urea concentration. The test specific detection rate for recovery of bacteriological pathogens was 79% for SI, 76% for ES, and 73% for BAL. Conclusion-SI offers safety advantages over BAL and may be a more representative airway outcome measurement in patients with CF.
ACCESSION #
13122894

 

Related Articles

  • The MIF Antagonist ISO-1 Attenuates Corticosteroid-Insensitive Inflammation and Airways Hyperresponsiveness in an Ozone-Induced Model of COPD. Russell, Kirsty E.; Chung, Kian Fan; Clarke, Colin J.; Durham, Andrew L.; Mallia, Patrick; Footitt, Joseph; Johnston, Sebastian L.; Barnes, Peter J.; Hall, Simon R.; Simpson, Karen D.; Starkey, Malcolm R.; Hansbro, Philip M.; Adcock, Ian M.; Wiegman, Coen H. // PLoS ONE;1/11/2016, Vol. 11 Issue 1, p1 

    Introduction: Macrophage migration inhibitory factor (MIF) is an inflammatory cytokine associated with acute and chronic inflammatory disorders and corticosteroid insensitivity. Its expression in the airways of patients with chronic obstructive pulmonary disease (COPD), a relatively steroid...

  • Granzyme K: a novel mediator in acute airway inflammation. Bratke, K.; Klug, A.; Julius, P.; Kuepper, M.; Lommatzsch, M.; Sparmann, G.; Luttmann, W.; Virchow, J. C. // Thorax;Nov2008, Vol. 63 Issue 11, p1006 

    Background: Granzymes are a subfamily of serine proteases involved in the pathogenesis of many inflammatory disorders. In contrast with granzyme A and B, the role of granzyme K (GrK) in human lung diseases is unknown. Therefore, the release and expression of GrK in allergic asthma, chronic...

  • Analysis of sputum in COPD. Maestrelli, P.; Richeldi, L.; Moretti, M.; Fabbri, L. M. // Thorax;Jun2001, Vol. 56 Issue 6, p420 

    It is therefore important to characterize and quantify inflammatory changes in the assessment of subjects with chronic obstructive pulmonary disease. A number of studies have suggested a pathogenetic role for airway inflammation in the induction of both chronic sputum production and chronic...

  • Budesonide mitigates pathological changes in animal model Of COPD through reducing neutrophil elastase expression. Lihong Wang; Bin Zhang; Zhu Li; Junhong Li; Qing Liu; Wuzhuang Sun // International Journal of Clinical & Experimental Medicine;2015, Vol. 8 Issue 4, p5227 

    Objectives: This study was conducted to investigate a molecular mechanism by which budesonide inhalation may mitigate pathological responses of cigarette smoke-induced COPD. Methods: Rats were exposed to air (control) and cigarette smoke (smoking) in the presence and absence of budesonide. Cell...

  • Lung density on high resolution computer tomography (HRCT) reflects degree of inflammation in smokers. Karimi, Reza; Tornling, Göran; Forsslund, Helena; Mikko, Mikael; Wheelock, Åsa M.; Nyrén, Sven; Sköld, Carl Magnus // Respiratory Research;2014, Vol. 15 Issue 2, p1 

    Background: Smokers have increased cell concentration in the lower respiratory tract indicating a chronic inflammatory state, which in some individuals may lead to development of chronic obstructive pulmonary disease (COPD). Computer tomography (CT) imaging provides means of quantifying...

  • Extracellular ATP in asthma airway inflammation. Parekh, D. // Thorax;Feb2008, Vol. 63 Issue 2, p140 

    The article discusses the significance of extracellular ATP and purinergic signalling as mediators in asthma airway inflammation. Binding of ATP to purinergic receptors using experimental mice models push inflammatory chemotaxis and bronchospasm. Management of exogenous ATP among mice rises...

  • Correlation between eicosanoids in bronchoalveolar lavage fluid and in exhaled breath condensate. Antczak, Adam; Piotrowski, Wojciech; Marczak, Jerzy; Ciebiada, Maciej; Gorski, Pawel; Barnes, Peter J. // Disease Markers;2011, Vol. 30 Issue 5, p213 

    Exhaled breath condensate (EBC) has been increasingly used as a new and non-invasive method to study airway inflammation. In this study we have compared the concentrations of lipid mediators in EBC with concentrations in bronchoalveolar lavage fluid (BALF). We included 37 patients undergoing...

  • IL-17A is essential to the development of elastase-induced pulmonary inflammation and emphysema in mice. Kurimoto, Etsuko; Miyahara, Nobuaki; Kanehiro, Arihiko; Waseda, Koichi; Taniguchi, Akihiko; Ikeda, Genyo; Koga, Hikari; Nishimori, Hisakazu; Tanimoto, Yasushi; Kataoka, Mikio; Iwakura, Yoichiro; Gelfand, Erwin W.; Tanimoto, Mitsune // Respiratory Research;Jan2013, Vol. 14 Issue 1, p1 

    Background: Pulmonary emphysema is characterized by alveolar destruction and persistent inflammation of the airways. Although IL-17A contributes to many chronic inflammatory diseases, it's role in the inflammatory response of elastase-induced emphysema remains unclear. Methods: In a model of...

  • Novel treatment for severe asthma. Bowden, Rachel // GP: General Practitioner;10/7/2005, p18 

    The article cites a British study which states that patients with severe asthma could benefit from treatment with tumor necrosis factor (TNF) blockers. The study found higher levels of the inflammatory cytokine TNF-alpha in patients with severe asthma than in patients with mild asthma. This...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics