TITLE

Impairment of intestinal intraepithelial lymphocytes in Id2 deficient mice

AUTHOR(S)
Kim, J-k; Takeuchi, M
PUB. DATE
April 2004
SOURCE
Gut;Apr2004, Vol. 53 Issue 4, p480
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Id2, an inhibitor of basic helix-loop-helix transcription factors, regulates cell differentiation. Id2-/- mice exhibit a variety of phenotypes in the immune system. Aims: In this study we investigated whether Id2 plays a role in intestinal intraepithelial lymphocytes (IELs), which constitute the main defense against pathogens in the intestinal tract. Methods: Flow cytometry and bone marrow transplantation were used to analyze and characterize subsets of IEls of Id2-/- mice. Gene expression was analyzed by real-time polymerase chain reaction. Intestinal barrier function was evaluated by treating mice with 5-fluorouracil (5-FU). Results: Among the four members of the Id gene family, ld2 was selectively expressed in all T cell subsets in the small intestinal IELs. Id2-/- mice showed alteration in the proportions of T cell subsets and a substantial reduction in the number of IEIs, especially those of the CD4+ and CD8αβ+ T cell subsets, indicating a more pronounced effect on thymus derived IEls. Expression of αE integrin was reduced in CD4+ and CD8αβ+ T cell subsets in (ELs of Id2-/- mice. IELs isolated from C57BL/6 mice reconstituted with ld2-/- bone marrow cells showed a similar phenotype to that of ld2-/- mice, indicating that the defects are intrinsic to bone marrow derived cells. Expression of genes encoding intestinal epithelial cell derived cytokines was reduced in ld2-/- mice. The 5-FU treatment revealed impaired intestinal barrier function of ld2-/- mice. Conclusions: The Id2 gene is essential for constituting the intestinal mucosal barrier, particularly with respect to IELs. Id2 null mutant mice may provide a good experimental model for studying the ontogeny of IEIs and intestinal inflammation and infection.
ACCESSION #
13120611

 

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