P-selectin upregulation in bleomycin induced lung injury in rats: effect of N-acelyl-L-cysteine

Serrano-Mollar, A.; Closa, D.; Cortijo, J.; Morcillo, E. J.; Prats, N.; Gironella, M.; Panés, J.; Roselló-Catafau, J.; Bulbena, O.
July 2002
Thorax;Jul2002, Vol. 57 Issue 7, p629
Academic Journal
Background: A number of adhesion molecules are involved in the process of neutrophil infiltration into the lung. P-selectin is one of these neutrophil-endothelial cell adhesion molecules. A study was undertaken to examine the involvement of P-selectin in the development of bleomycin induced inflammation and the ability of N-acetyl-L-cysteine to reduce the potential expression of this selectin in rats. Methods: N-acetyl-L-cysteine (3 mmol/kg po) was administered daily for seven days prior to bleomycm administration (2.5 U/kg). The kinetics of P-selectin expression and the effect of N-acetyl-L-cysteine after bleomycmn treatment were measured using radiolabelled antibodies. P-selectin localisation was evaluated by immunohistochemistry and neutrophil infiltration was assessed by myeloperoxidase activity. Results: Bleomycin administration resulted line at 3 hours. Myeloperoxidase activity administration that lasted for 3 days. increases. Conclusion: Upregulation of P-selectin in the Jung is associated with neutrophil recruitment in response to bleomycmn. The beneficial effect of N-acetyl-L-cysteine on bleomycin induced lung injury may be explained in part by the prevention of neutrophil recruitment in the inflammatory stage of the disease.


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