TITLE

A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor a coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRA

AUTHOR(S)
Watanabe, Michiko; Yanagisawa, Junn; Kitagawa, Hirochika; Takeyama, Ken-ichi; Ogawa, Satoko; Arao, Yukitomo; Suzawa, Miyuki; Kobayashi, Yoko; Yano, Tetsu; Yoshikawa, Hiroyuki; Masuhiro, Yoshikazu; Kato, Shigeaki
PUB. DATE
March 2001
SOURCE
EMBO Journal;3/15/2001, Vol. 20 Issue 6, p1341
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
One class of the nuclear receptor AF-2 coactivator complexes contains the SRC-1/TIF2 family, CBP/p300 and an RNA coactivator, SRA. We identified a subfamily of RNA-binding DEAD-box proteins (p72/p68) as a human estrogen receptor a (hERa) coactivator in the complex containing these factors. p72/p68 interacted with both the AD2 of any SRC-1/TIF2 family protein and the hERa A/B domain, but not with any other nuclear receptor tested. p72/p68, TIF2 (SRC-1) and SRA were co-immunoprecipitated with estrogen-bound hERa in MCF7 cells and in partially purified complexes associated with hERa from HeLa nuclear extracts. Estrogen induced co-localization of p72 with hERa and TIF2 in the nucleus. The presence of p72/p68 potentiated the estrogen-induced expression of the endogenous pS2 gene in MCF7 cells. In a transient expression assay, a combination of p72/p68 with SRA and one TIF2 brought an ultimate synergism to the estrogen-induced transactivation of hERa. These findings indicate that p72/p68 acts as an ER subtype-selective coactivator through ERa AF-1 by associating with the coactivator complex to bind its AF-2 through direct binding with SRA and the SRC-1/TIF2 family proteins.
ACCESSION #
12954972

 

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