Directed neutrophil migration to IL-8 is increased in cystic fibrosis: a study of the effect of erythromycin

Brennan, S.; Cooper, D.; Sly, P. D.
January 2001
Thorax;Jan2001, Vol. 56 Issue 1, p62
Academic Journal
Background -- The aim of this study was to compare neutrophil migration in cystic fibrosis (CF) and non-CF populations and to investigate the effect of erythromycin on directed migration of neutrophils (PMNs) in CE Methods -- PMNs were isolated and their migratory capacity in response to interleukin-8 (IL-8) or f-Met-Leu-Phe (tMLP) in the presence or absence of erythromycin (1-100 μg/ml) was assessed. Results -- CF derived PMNs showed significantly increased migration to IL-8 but not to fMLP compared with non-CF PMNs. Erythromycin had no significant effect on migration responses to IL-S and in vitro exposure of PMNs to erythromycin had no effect. Conclusions -- CF derived PMNs show higher migratory responsiveness to IL-S but not to tMLP, suggesting that CF PMNs may be "primed" to IL-8 which is significantly increased in CF serum compared with non-CF serum. Treatment with erythromycin had no significant effect on PMN migration in vitro.


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