β2 adrenoceptor promoter polymorphisms: extended haplotypes and functional effects in peripheral blood mononuclear cells

Lipworth, B.; Koppelman, G. H.; Wheatley, A. P.; Le Jeune, I.; Coutie, W.; Meurs, H.; Kauffman, H. F.; Postma, D. S.; Hall, I. P.
January 2002
Thorax;Jan2002, Vol. 57 Issue 1, p61
Academic Journal
Background: The β2 adrenoceptor and its 5' untranslated region contain a number of genetic variants. The aim of this study was to investigate the potential for genetic variation at this locus to influence the expression of β2 adrenoceptors on circulating peripheral blood mononuclear cells (PBMCs). Methods: Genotype was determined in 96 individuals with asthma for four polymorphisms at the β2 adrenoceptor locus. β2 adrenoceptor binding and cyclic AMP responses to isoprenaline in PBMCs were determined and the relationship between genotype/haplotype and β2 adrenoceptor expression and response to isoprenaline examined. Results: β2 adrenoceptor promoter polymorphisms were found to be common in white subjects. Strong linkage disequilibrium exists across this locus, resulting in the occurrence of several common hoplotypes. No single polymorphism or haplotype was correlated with the level of β2 adrenoceptor expression or cyclic AMP responses to isoprenaline in vitro. Conclusion: β2 adrenoceptor polymorphisms, when considered in isolation or by extended haplosypes, do not determine the basal level of expression or coupling of β2 adrenoceptors in PBMCs from asthmatic subjects.


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