Association of interleukin 1B gene polymorphism and gastric cancers in high and low prevalence regions in China

Zeng, Z. -R.; Hu, P. -J.; Hu, S.; Pang, R. -P.; Chen, M. -H.; Ng, M.; Sung, J. J. Y.
December 2003
Gut;Dec2003, Vol. 52 Issue 12, p1684
Academic Journal
Aim: Our aim was to study the relationship between interleukin 1B (IL-1B) polymorphism, Helicobacter pylon infection, and gastric cancer in high prevalent (Shanxi) and low prevalent (Guangdong) regions in China. Method: Genomic DNA was extracted from peripheral blood of 192 healthy volunteers, 84 gastric cancer patients from Guangdong and 169 healthy volunteers, and 86 gastric cancer patients from Shanxi. Polymorphisms in IL-1B that encodes IL-1β and IL-lRN that encodes IL-1 receptor antagonist were analysed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). These polymorphic sites include promoter regions of IL-1B at positions +3954, -511 (C-T transition), and -31 (T-C transition), and IL-1RN variable tandem repeats. Results: In the low prevalence region, the frequencies of the IL-1B +3954 T/T and IL-1RN *2/*2 genotypes were similar. IL-1B -511 T/T genotype frequency was significantly higher among patients with gastric cancer (25.0%) than control subjects (12.5%) (χ²=6.7, p=0.01). In the high prevalence region, the frequencies of the IL-1B+3954 T/T and -511T/T genotypes and the IL-1RN *2/*2 genotype in the cancer and control groups were similar. IL- 1B -31C/C genotype frequency was significantly higher among patients with gastric cancer (90.0%) than controls (78.0%) (χ²=5.0, p=0.025). Compared with the low prevalence region, control subjects from the high prevalence region had a higher frequency of the IL-1B -511T/T genotype (23.0% v 12.5%; χ²=7.0, p<0.008). While H pylori infection alone had only a modest effect on the risk of gastric cancer development (odds ratio (OR) 5.0 (95% confidence interval (CI) 1.5-16.3)), combined with the IL-1B -511T/T genotype the risk was markedly elevated (OR 17.1, 95% Cl 3.8-76.4). Conclusion: IL-1B -511T/T genotypes are associated with gastric cancer in China. The effect of IL-1B polymorphism is less obvious in areas of high prevalence for gastric cancer.


Related Articles

  • Evaluating Helicobacter Pylori FlaA in Clinical Isolates from Alzahra Hospital, Isfahan, Iran. Madhi, Masoomeh; Moghim, Sharareh; Pursina, Farkhondeh; Adibi, Peyman; Khademi, Farzad; Farajzadegan, Ziba; Safaei, Hajieh Ghasemian // Journal of Isfahan Medical School;Apr2013, Vol. 31 Issue 223, p1 

    Background: This study aimed to survey the presence of flagellin gene flaA in isolated Helicobacter pylori. Methods: One hundred gastric biopsies were collected by a gastroenterologist and inoculated in enrichment medium under microaerophilic conditions. DNA was extracted and polymerase chain...

  • Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP) analysis. Gopaul, Krishna K.; Koylass, Mark S.; Smith, Catherine J.; Whatmore, Adrian M. // BMC Microbiology;2008, Vol. 8, Special section p1 

    Background: Brucellosis, caused by members of the genus Brucella, remains one of the world's major zoonotic diseases. Six species have classically been recognised within the family Brucella largely based on a combination of classical microbiology and host specificity, although more recently...

  • Development of a Highly Sensitive Method for Detection of Clarithromycin-Resistant Helicobacter pylori from Human Feces. Rimbara, Emiko; Noguchi, Norihisa; Yamaguchi, Tai; Narui, Koji; Kawai, Takashi; Sasatsu, Masanori // Current Microbiology;Jul2005, Vol. 51 Issue 1, p1 

    Gastric infection of clarithromycin (CAM)-resistant Helicobacter pylori is one of the major causes of failure to eradicate this organism. A noninvasive and useful method for the detection of CAM-resistant H. pylori from human feces by restriction fragment length polymorphism (RFLP)-nested...

  • −607 C/A polymorphism in the promoter of IL-18 gene is associated with 2 h post-loading plasma glucose level in Chinese. Yun Huang; Min Xu; Jie Hong; Weiqiong Gu; Yufang Bi; Xiaoying Li // Endocrine (1355008X);Jun2010, Vol. 37 Issue 3, p507 

    The purpose of this study is to find out whether the −607 C/A polymorphism in IL-18 gene promoter will affect serum IL-18 concentrations and glucose metabolism in Chinese subjects. In 232 patients with impaired glucose regulation (IGR) or type 2 diabetes and 212 subjects of normal glucose...

  • Two polymorphisms within interleukin-3 (hIL3) gene detected by mismatch PCR/RFLP. Nakashima, H; Otsuka, T; Ohba, Y; Akahoshi, M; Nagano, S; Ogami, E; Arinobu, Y; Miyake, K; Inoue, Y; Niiro, H; Kaji, Y; Niho, Y // Genes & Immunity;Nov99, Vol. 1 Issue 2, p156 

    Two alleles of IL-3 have been reported to GenBank (GenBank M14743, M20137). The sequence difference between these two alleles is at the first nucleotide of the 27th codon (the 131st nucleotide from the initiation site): thymine and cytosine, and leading the amino acid difference: proline and...

  • Tularemia Outbreak, Bulgaria, 1997-2005. Kantardjiev, Todor; Ivanov, Ivan; Velinov, Tzvetan; Padeshki, Plamen; Popov, Boris; Nenova, Roumiana; Mincheff, Milcho // Emerging Infectious Diseases;Apr2006, Vol. 12 Issue 4, p678 

    The 1997-2005 tularemia outbreak in Bulgaria affected 285 people. Ten strains were isolated from humans, a tick, a hare, and water. Amplified fragment length polymorphism typing of the present isolates and of the strain isolated in 1962 suggests that a new genetic variant caused the outbreak.

  • Diagnostic approaches for oculoglandular tularemia: advantages of PCR. Kantardjiev, Todor; Padeshki, Plamen; Ivanov, Ivan N. // British Journal of Ophthalmology;Sep2007, Vol. 91 Issue 9, p1206 

    Aim: The authors describe a diagnostic approach that proved to be particularly valuable in rare cases of ocular tularemia registered during the tularemia outbreak in 1997-2005 in Bulgaria. The authors describe the laboratory findings and diagnosis of four cases with an oculoglandular form of...

  • Respuesta al tratamiento de brucelosis en niños. Briones-Lara, Evangelina; Palacios-Saucedo, Gerardo del C.; Martínez-Vázquez, Irma Olivia; Morales-Loredo, Alberto; Bilbao-Chávez, Leticia del Pilar // Revista Medica del IMSS;nov/dic2007, Vol. 45 Issue 6, p615 

    Introduction: brucellosis poses a significant public health problem and requires meticulous diagnosis; the outcome has frequent relapses even when the treatment is appropriate. Objective: To evaluate the response to the treatment in children with brucellosis by means of Huddleson...

  • Validation of a same-day real-time PCR method for screening of meat and carcass swabs for Salmonella. Löfström, Charlotta; Krause, Michael; Josefsen, Mathilde H.; Hansen, Flemming; Hoorfar, Jeffrey // BMC Microbiology;2009, Vol. 9, Special section p1 

    Background: One of the major sources of human Salmonella infections is meat. Therefore, efficient and rapid monitoring of Salmonella in the meat production chain is necessary. Validation of alternative methods is needed to prove that the performance is equal to established methods. Very few of...


Read the Article


Sign out of this library

Other Topics