Highly sensitive ac nanocalorimeter for microliter-scale liquids or biological samples

Garden, J.-L.; Château, E.; Chaussy, J.
May 2004
Applied Physics Letters;5/3/2004, Vol. 84 Issue 18, p3597
Academic Journal
We developed an ultrasensitive ac nanocalorimeter for use with biological liquids by means of microfabrication technologies. The volume of the cell measurement is only 5 μL. This nanocalorimeter, tested on deionized water, allows the measurement of heat capacity variation above ±150 nJ/K (resolution of ±5×10-6) with an operating temperature ranging from -20 to 120 °C and a stability of 100 μK. Its use is demonstrated on diluted lysosyme solution measured at the frequency of 3 Hz. At 3 Hz, this ac calorimeter gives only the variation of the heat capacity during the denaturation, which allows complementary thermodynamic investigations as regards to classical differential scanning calorimetry measurements. © 2004 American Institute of Physics.


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