TITLE

Decreased peroxynitrite inhibitory activity in induced sputum in patients with bronchial asthma

AUTHOR(S)
Kanazawa, H.; Shiraishi, S.; Hirata, K.; Yoshikawa, J.
PUB. DATE
June 2002
SOURCE
Thorax;Jun2002, Vol. 57 Issue 6, p509
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: The production of peroxynitrite an extremely potent oxidant, is increased in inflammatory lung disease. It is therefore important to measure antioxidant activity against peroxynitrite in epithelial lining fluid to examine the physiological effects of peroxynitrite in the airways of patients with asthma. This study was designed to determine whether peroxynitrite inhibitory activity in induced sputum is correlated with clinical characteristics and airway inflammatory indices in asthmatic patients. Methods: Inflammatory indices were measured in induced sputum from 25 patients with asthma and 1 2 normal control subjects. Peroxynitrite inhibitory activity was also measured by monitoring rhodamine formation in sputum samples. Results: Peroxynitrite inhibitory activity in induced sputum was significantly lower in asthmatic patients (52.4 (24.5)%) than in normal control subjects (92.1 (3.9)%, p<0.0001) Its activity was significantly correlated with forced expiratory volume in 1 second (FEY1) % predicted (r=0.774, p<0.0001) and bronchial hyperreactivity to methacholine (r=0.464, p=0.023). There was a significant negative correlation between peroxynitrite inhibitory activity and the degree of eosinophilic airway inflammation (% eosinophils r=-0.758, p<0.001; eosinophil cationic protein, r=-0.780 p<0.0001). Conclusions: Decreased peroxynitrite inhibitory activity occurs in induced sputum of asthmatic patients. Since even in patients with stable asthma the airway lining fluid lacks peroxynitrite inhibitory activity, large amounts of peroxynitrite, which are further increased during an acute asthma attack, would not be completely inactivated and asthmatic airways might have markedly increased susceptibility to peroxynitrite induced airway injury.
ACCESSION #
12915583

 

Related Articles

  • Simplified detection of eosinophils in induced sputum. Fireman, Elizabeth; Toledano, Brenda; Buchner, Nina; Stark, Moshe; Schwarz, Yehuda // Inflammation Research;Aug2011, Vol. 60 Issue 8, p745 

    Objective and design: The induction of sputum is a safe, noninvasive method of studying airway inflammation in asthma, but the method of analyzing the samples is laborious and requires well-trained technicians using highly specialized laboratory equipment. We introduce a shorter and simpler...

  • Independent Nurse: Journals Watch - Eosinophil predicts asthma control after stopping steroids.  // GP: General Practitioner;5/20/2005, p90 

    The article focuses on the asthma control. The U.S. researchers monitored 164 patients with mild to moderate asthma for 16 weeks. They found sputum eosinophil counts measured two weeks after stopping steroid use predicted future asthma deterioration.

  • Theophylline Inhibits Integrin-Dependent Eosinophil Superoxide Production. Piacentini, Giorgio L.; Peroni, Diego G.; Bodini, Alessandro; Vicentini, Lucia; Baraldi, Eugenio; Boner, Attilio L.; Chatzimichail, Atanasio; Berton, Giorgio // Allergy & Asthma Proceedings;Jan/Feb2005, Vol. 26 Issue 1, p47 

    Theophylline has been proposed as a drug that is able to reduce eosinophil activation in asthma. We tested the hypothesis that it can interfere with the integrin-mediated stimulation of eosinophil function. Eosinophils from healthy donors were triggered by monoclonal antibodies to β1- and...

  • Correlation of the severity of atopic dermatitis with absolute eosinophil counts in peripheral blood and serum IgE levels. Dhar, Sandipan; Malakar, Rajib; Chattopadhyay, Soumen; Dhar, Subhra; Banerjee, Raghubir; Ghosh, Apurba // Indian Journal of Dermatology, Venereology & Leprology;Jul/Aug2005, Vol. 71 Issue 4, p246 

    Background: Although a number of epidemiological studies, showing incidence and prevalence of atopic dermatitis, were available, scant attention has been paid to the correlation between the parameters of the disease like severity, absolute eosinophil count and lgE level, which has been known to...

  • S�ndromes pulmonares eosinof�licos. Jim�nez, Abraham Emilio Reyes; Guti�rrez, Jorge Gonz�lez; G�mez, Ixchel Landgrave; D'Franchis, Luis Javier Castro; Galv�n, Alma Cristina P�rez; L�pez, Fernando Rogelio Espinosa // Medicina Interna de Mexico;nov/dic2009, Vol. 25 Issue 6, p461 

    Although the eosinophiles are normal lung components, several lung eosinophilic syndromes are characterized by an increased number of eosinophiles in peripheral blood, pulmonary tissue, sputum, or bronchoalveolar lavage. These lung eosinophilic syndromes are generally characterized by...

  • Reduction in sputum neutrophil and eosinophil numbers by the PDE4 inhibitor roflumilast in patients with COPD. Grootendorst, Diana C.; Gauw, Stefanie A.; Verhoosel, Renate M.; Sterk, Peter J.; Hospers, Jeannette J.; Bredenbröker, Dirk; Bethke, Thomas D.; Hiemstra, Pieter S.; Rabe, Klaus F. // Thorax;Dec2007, Vol. 62 Issue 12, p1081 

    Background: Roflumilast is a targeted oral once-daily administered phosphodiesterase 4 (PDE4) inhibitor with clinical efficacy in chronic obstructive pulmonary disease (COPD). Results from in vitro studies with roflumilast indicate that it has anti-inflammatory properties that may be applicable...

  • THE ASTHMA TRAP. Corbett, Sara // Mother Jones;Mar/Apr2005, Vol. 30 Issue 2, p58 

    Presents information on the prevalence of asthma in Chicago, Illinois. Pathogenesis of asthma; Causes of asthma attack; Role of environmental changes in the increasing number of people with asthma.

  • The Prevalence of Asthma, Allergic Rhinitis, and Atopy in Antalya, Turkey. Dinmezel, Semra; Ogus, Candan; Erengin, Hakan; Cilli, Aykut; Ozbudak, Omer; Ozdemir, Tulay // Allergy & Asthma Proceedings;Sep/Oct2005, Vol. 26 Issue 5, p403 

    Bronchial asthma is a common chronic inflammatory disorder of the airways that causes serious morbidity and mortality. The prevalence of the disorder has been increasing, especially in developed countries. The population-based asthma prevalence studies provide data from Ankara, Istanbul,...

  • More Than Half of US Asthma Cases May Be Linked to Atopy.  // Pulmonary Reviews;Nov2007, Vol. 12 Issue 11, p16 

    The article discusses research being done on asthma cases in the U.S. attributed to atopy. It references a study by Darryl C. Zeldin and colleagues published in the September 21, 2007 issue of the "Journal of Allergy and Clinical Immunology." NIH researchers found that more than half of current...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics