Genome Wide Identification and Expression Profiling of SWEET Genes Family Reveals Its Role During Plasmodiophora brassicae-Induced Formation of Clubroot in Brassica rapa

Li, Hong; Li, Xiaonan; Xuan, Yuanhu; Jiang, Jing; Wei, Yangdou; Piao, Zhongyun
February 2018
Frontiers in Plant Science;2/28/2018, p1
Academic Journal
Plasmodiophora brassicae is a soil borne pathogen and the causal agent of clubroot, a devastating disease of Brassica crops. The pathogen lives inside roots, and hijacks nutrients from the host plants. It is suggested that clubroot galls created an additional nutrient sink in infected roots. However, the molecular mechanism underlying P. brassicae infection and sugar transport is unclear. Here, we analyzed sugar contents in leaves and roots before and after P. brassicae infection using a pair of Chinese cabbage near-isogenic lines (NILs), carrying either a clubroot resistant (CR) or susceptible (CS) allele at the CRb locus. P. brassicae infection caused significant increase of glucose and fructose contents in the root of CS-NIL compared to CR-NIL, suggesting that sugar translocation and P. brassicae growth are closely related. Among 32 B. rapa SWEET homologs, several BrSWEETs belonging to Clade I and III were significantly up-regulated, especially in CS-NIL upon P. brassicae infection. Moreover, Arabidopsis sweet11 mutant exhibited slower gall formation compared to the wild-type plants. Our studies suggest that P. brassicae infection probably triggers active sugar translocation between the sugar producing tissues and the clubbed tissues, and the SWEET family genes are involved in this process.


Related Articles

  • A Genome-Wide Association Study Reveals New Loci for Resistance to Clubroot Disease in Brassica napus. Lixia Li; Yujie Luo; Biyun Chen; Kun Xu; Fugui Zhang; Hao Li; Qian Huang; Xin Xiao; Tianyao Zhang; Jihong Hu; Feng Li; Xiaoming Wu // Frontiers in Plant Science;9/30/2016, Vol. 7, p1 

    Rapeseed (Brassica napus L.) is one of the most important oil crops in the world. However, the yield and quality of rapeseed were largely decreased by clubroot (Plasmodiophora brassicae Woronin). Therefore, it is of great importance for screening more resistant germplasms or genes and improving...

  • What can we learn from clubroots: alterations in host roots and hormone homeostasis caused by Plasmodiophora brassicae. Jutta Ludwig-Müller; Astrid Schuller // European Journal of Plant Pathology;Jul2008, Vol. 121 Issue 3, p291 

    Abstract  The clubroot disease of cruciferous crops is caused by an obligate biotrophic protist, Plasmodiophora brassicae. The disease is characterized by the development of large root galls accompanied by changes in source-sink relations and the hormonal balance within the plant. Since...

  • Science into Practice Glubroot control in propagation.  // Horticulture Week;8/30/2013, p45 

    The article reports on the effective control of clubroots or Plasmodiophora brassicae, the most serious disease affecting brassica crops in Great Britain with the use of heat and disinfectants which will minimise the risk of disease transfer to soil.

  • Shotgun Label-free Proteomic Analysis of Clubroot (Plasmodiophora brassicae) Resistance Conferred by the Gene Rcr1 in Brassica rapa. Song Tao; Mingguang Chu; Lahlali, Rachid; Fengqun Yu; Peng, Gary // Frontiers in Plant Science;7/11/2016, p1 

    Clubroot, caused by the plasmodiophorid pathogen Plasmodiophora brassicae, is one of the most serious diseases on Brassica crops worldwide and a major threat to canola production in western Canada. Host resistance is the key strategy for clubroot management on canola. Several clubroot resistance...

  • Integrating long noncoding RNAs and mRNAs expression profiles of response to Plasmodiophora brassicae infection in Pakchoi (Brassica campestris ssp. chinensis Makino). Zhu, Hongfang; Li, Xiaofeng; Xi, Dandan; Zhai, Wen; Zhang, Zhaohui; Zhu, Yuying // PLoS ONE;12/5/2019, Vol. 14 Issue 12, p1 

    The biotrophic protist Plasmodiophora brassicae causes serious damage to Brassicaceae crops grown worldwide. However, the molecular mechanism of the Brassica rapa response remains has not been determined. Long noncoding RNA and mRNA expression profiles in response to Plasmodiophora brassicae...

  • Variability in Resistance to Clubroot in European Cauliflower Cultivars. KOPECKÝ, PAVEL; DOLEŽALOVÁ, IVANA; DUCHOSLAV, MARTIN; DUŠEK, KAREL // Plant Protection Science;2012, Vol. 48 Issue 4, p156 

    Fifty genotypes of cauliflower (Brassica oleracea var. botrytis) were evaluated for resistance to clubroot disease (Plasmodiophora brassicae Wor.) under controlled conditions in a plant growth chamber. The cultivars with the highest resistance were Brilant, Agora, and Bora, while the most...

  • Plasmodiophora brassicae in its Environment. Dixon, Geoffrey R. // Journal of Plant Growth Regulation;Sep2009, Vol. 28 Issue 3, p212 

    Plasmodiophora brassicae Wor. is viewed in this article from the standpoint of a highly evolved and successful organism, well fitted for the ecological niche that it occupies. Physical, chemical, and biological components of the soil environment are discussed in relation to their effects on the...

  • Clubroot Of Crucifers. Hansen, Mary Ann // American Vegetable Grower;Mar2004, Vol. 52 Issue 3, p7 

    Presents information on clubroot of crucifers also known as Plasmodiophora brassicae. Crops affected by the disease; Survival and spread; Management.

  • OSR beware! Clubroot cometh. Dixon, Geoff // Crops;6/23/2007, p42 

    The article presents information on the serious effects of clubroot disease on crop plants, particularly on the oilseed rape. Clubroot disease results from infection by the soil-borne microbe Plasmodiophora brassicae. It is stated that clubroot is public enemy number one for broccoli, Brussels...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics