Enhanced production of a diastereomer type of mannosylerythritol lipid-B by the basidiomycetous yeast Pseudozyma tsukubaensis expressing lipase genes from Pseudozyma antarctica

Saika, Azusa; Koike, Hideaki; Yamamoto, Shuhei; Kishimoto, Takahide; Morita, Tomotake
December 2017
Applied Microbiology & Biotechnology;Dec2017, Vol. 101 Issue 23/24, p8345
Academic Journal
Basidiomycetous yeasts in the genus Pseudozyma are known to produce extracellular glycolipids called mannosylerythritol lipids (MELs). Pseudozyma tsukubaensis produces a large amount of MEL-B using olive oil as the sole carbon source (> 70 g/L production). The MEL-B produced by P. tsukubaensis is a diastereomer type of MEL-B, which consists of 4- O-β- d-mannopyranosyl-(2 R,3 S)-erythritol as a sugar moiety, in contrast to the conventional type of MELs produced by P. antarctica, which contain 4- O-β- d mannopyranosyl-(2 S,3 R)-erythritol. In this study, we attempted to increase the production of the diastereomer type of MEL-B in P. tsukubaensis 1E5 by introducing the genes encoding two lipases, PaLIPAp ( PaLIPA) and PaLIPBp ( PaLIPB) from P. antarctica T-34. Strain 1E5 expressing PaLIPA exhibited higher lipase activity than the strain possessing an empty vector, which was used as a negative control. Strains of 1E5 expressing PaLIPA or PaLIPB showed 1.9- and 1.6-fold higher MEL-B production than the negative control strain, respectively, and oil consumption was also accelerated by the introduction of these lipase genes. MEL-B production was estimated using time course analysis in the recombinant strains. Strain 1E5 expressing PaLIPA produced 37.0 ± 1.2 g/L of MEL-B within 4 days of cultivation, whereas the strain expressing an empty vector produced 22.1 ± 7.5 g/L in this time. Overexpression of PaLIPA increased MEL-B production by P. tsukubaensis strain 1E5 from olive oil as carbon source by more than 1.7-fold.


Related Articles

  • Production and characterization of a glycolipid biosurfactant from Bacillus megaterium using economically cheaper sources. R. Thavasi; S. Jayalakshmi; T. Balasubramanian; Ibrahim Banat // World Journal of Microbiology & Biotechnology;Jul2008, Vol. 24 Issue 7, p917 

    Abstract  Criteria selected for screening of biosurfactant production by Bacillus megaterium were hemolytic assay, bacterial cell hydrophobicity and the drop-collapse test. The data on hemolytic activity, bacterial cell adherence with crude oil and the drop-collapse test confirmed the...

  • Extracellular production of a glycolipid biosurfactant, mannosylerythritol lipid, from Candida antarctica. Kim, Hee-Sik; Jeon, Jong-Woon; Lee, Hong-Won; Park, Yong-II; Seo, Weon-Taek; Oh, Hee-Mock; Katsuragi, Tohoru; Tani, Yoshiki; Yoon, Byung-Dae // Biotechnology Letters;Feb2002, Vol. 24 Issue 3, p225 

    Candida antarctica (sp. SY16) required a vegetable oil as the carbon source to produce a biosurfactant, mannosylerythritol lipid (MEL-SY16). Biosurfactant production was 31 g l-1 after 7 days in a batch culture and was not growth associated. In a two-stage culture, glycerol and oleic acid were...

  • Biosurfactants: a sustainable replacement for chemical surfactants? Marchant, Roger; Banat, Ibrahim // Biotechnology Letters;Sep2012, Vol. 34 Issue 9, p1597 

    Glycolipid biosurfactants produced by bacteria and yeasts provide significant opportunities to replace chemical surfactants with sustainable biologically produced alternatives in bulk commercial products such as laundry detergents and surface cleaners. Sophorolipids are already available in...

  • Pseudomonas aeruginosa rhamnolipids: biosynthesis and potential applications. Maier, R. M.; Soberón-Chávez, G. // Applied Microbiology & Biotechnology;Nov2000, Vol. 54 Issue 5, p625 

    Pseudomonas aeruginosa produces and secretes rhamnose-containing glycolipid biosurfactants called rhamnolipids. This review describes rhamnolipid biosynthesis and potential industrial and environmental applications of rhamnolipids. Rhamnolipid production is dependent on central metabolic...

  • An update on the use of unconventional substrates for biosurfactant production and their new applications. Makkar, R. S.; Cameotra, S. S. // Applied Microbiology & Biotechnology;Mar2002, Vol. 58 Issue 4, p428 

    Biosurfactants are valuable microbial amphiphilic molecules with effective surface-active and biological properties applicable to several industries and processes. Microbes synthesize them, especially during growth on water-immiscible substrates, providing an alternative to chemically prepared...

  • Microbial conversion of n-alkanes into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma (Candida antarctica). Kitamoto, Dai; Ikegami, Toru; Suzuki, Gaby Tiemi; Sasaki, Akira; Takeyama, Yu-ichiro; Idemoto, Yasushi; Koura, Nobuyuki; Yanagishita, Hiroshi // Biotechnology Letters;Oct2001, Vol. 23 Issue 20, p1709 

    n-Alkanes ranging from C12 to C18 were converted into glycolipid biosurfactants, mannosylerythritol lipids (MEL), by resting cells of Pseudozyma (Candida) antarctica T-34. The highest yield (0.87 g g-1 substrate) was obtained from 6% (v/v) of n-octadecane after 7 days reaction. The amount of MEL...

  • Conversion of cellulosic materials into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma spp. under SHF and SSF processes. Faria, Nuno Torres; Santos, Marisa; Ferreira, Carla; Marques, Susana; Ferreira, Frederico Castelo; Fonseca, César // Microbial Cell Factories;2014, Vol. 13 Issue 1, p1 

    Background Mannosylerythritol lipids (MEL) are glycolipids with unique biosurfactant properties and are produced by Pseudozyma spp. from different substrates, preferably vegetable oils, but also sugars, glycerol or hydrocarbons. However, solvent intensive downstream processing and the relatively...

  • A new assay for rhamnolipid detection-important virulence factors of Pseudomonas aeruginosa. Laabei, Maisem; Jamieson, William; Lewis, Simon; Diggle, Stephen; Jenkins, A. // Applied Microbiology & Biotechnology;Aug2014, Vol. 98 Issue 16, p7199 

    Rhamnolipids (RLs) are heterogeneous glycolipid molecules that are composed of one or two l-rhamnose sugars and one or two β-hydroxy fatty acids, which can vary in their length and branch size. They are biosurfactants, predominantly produced by Pseudomonas aeruginosa and are important...

  • Sophorolipids production by Candida bombicola using dairy industry wastewater. Daverey, A.; Pakshirajan, K.; Sumalatha, S. // Clean Technologies & Environmental Policy;Jun2011, Vol. 13 Issue 3, p481 

    Wastewater obtained from a local dairy industry was simultaneously utilized and treated for the production of sophorolipids (SLs), a glycolipids type of biosurfactant, by the yeast Candida bombicola. The dairy wastewater, after initial characterization, was utilized as media with or without...

  • Discovery of Pseudozyma rugulosa NBRC 10877 as a novel producer of the glycolipid biosurfactants, mannosylerythritol lipids, based on rDNA sequence.  // Applied Microbiology & Biotechnology;Nov2006, Vol. 73 Issue 2, p305 

    The search for a novel producer of glycolipid biosurfactants, mannosylerythritol lipids (MEL) was undertaken based on the analysis of ribosomal DNA sequences on the yeast strains of the genus Psewhzyma. Pseudozyma rugulosa NBRC 10877 was found to produce a large amount of glycolipids from...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics