TITLE

Targeted next-generation sequencing for analyzing the genetic alterations in atypical adenomatous hyperplasia and adenocarcinoma in situ

AUTHOR(S)
Xu, Xuan; Li, Na; Zhao, Ruiying; Zhu, Lei; Shao, Jinchen; Zhang, Jie
PUB. DATE
December 2017
SOURCE
Journal of Cancer Research & Clinical Oncology;Dec2017, Vol. 143 Issue 12, p2447
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: Atypical adenomatous hyperplasia (AAH) and adenocarcinoma in situ (AIS) have been defined as preinvasive pulmonary adenocarcinoma lesions according to the 2015 World Health Organization lung adenocarcinoma classification. We aimed to search for the most common gene mutations in patients with AAH and AIS and investigate the distinctions between the two groups at the molecular level. Methods: We performed targeted next-generation sequencing on 18 cases with AAH and 28 cases with AIS to screen for mutations with the Ion Torrent Oncomine Solid Tumor DNA panel. ALK and ROS1 fusions were detected by real-time PCR. Results: Forty-six mutations were identified in 29 cases (76.1%), including 9 (50%) of 18 cases with AAH and 20 (71.4%) of 28 cases with AIS, in the following genes: EGFR, BRAF, KRAS, ERBB2, TP53, and FGFR3. The mutations in EGFR, BRAF, KRAS, ERBB2, and TP53 genes were more common in AIS lesions than in AAH lesions, whereas the FGFR3 gene was more frequently mutated in AAH compared to AIS. ALK and ROS1 fusions were not detected in any of the lesions. Conclusions: Based on the molecular evidence, the proposal that AAH and AIS are preinvasive lesions of pulmonary adenocarcinomas is of great significance, and it is necessary to distinguish AAH from AIS. Our study provided insights into the genetic alterations in the early stage of lung adenocarcinoma, which could be beneficial for the pathologic diagnosis and early detection of these lesions.
ACCESSION #
126306479

 

Related Articles

  • Sialadenoma papilliferum with potentially malignant features. Ide, F.; Kikuchi, K.; Kusama, K.; Kanazawa, H. // Journal of Clinical Pathology;Apr2010, Vol. 63 Issue 4, p362 

    Sialadenoma papilliferum (SP) is a benign non-invasive lesion with slow and limited growth. This report describes an unusual SP that enlarged rapidly and presented as an extensive intraoral tumour highly suspicious for verrucous carcinoma. Radiographic resorption of the underlying bone was...

  • Natural history of endometrial hyperplasia : Study of 77 patients. Tabata, T.; Yamawaki, T.; Yabana, T.; Ida, M.; Nishimura, K.; Nose, Y. // Archives of Gynecology & Obstetrics;May2001, Vol. 265 Issue 2, p85 

    Seventy-seven patients with endometrial hyperplasia, 48 with simple hyperplasia without atypia (SH), 17 with complex hyperplasia without atypia (CH), one with simple hyperplasia with atypia (SHA), and 11 with complex hyperplasia with atypia (CHA) were prospectively followed-up by total curettage...

  • Periacinar retraction clefling in proliferative prostatic atrophy and prostatic adenocarcinoma. Ulamec, Monika; Tomas, Davor; Ensinger, Christian; Ćupić, Hrvoje; Belicza, Mladen; Mikuz, Gregor; Krušlin, Božo // Journal of Clinical Pathology;Oct2007, Vol. 60 Issue 10, p1098 

    Aims: To evaluate the presence and extent of periacinar retraction clefting in proliferative prostatic atrophy and carcinoma in radical prostatectomy specimens. Methods: Atrophic foci and neoplastic glands were analysed in specimens from 50 patients who underwent radical prostatectomy. Analysed...

  • Long-term effects of surgical correction of adrenal hyperplasia and adenoma causing primary aldosteronism. Hennings, J.; Andreasson, S.; Botling, J.; Hägg, A.; Sundin, A.; Hellman, P. // Langenbeck's Archives of Surgery;Feb2010, Vol. 395 Issue 2, p133 

    The purpose of this is to study long-time results of surgery for primary aldosteronism. Thirty patients operated on for primary aldosteronism were followed for an average of 7 years. All but five required potassium substitution. Systolic as well as diastolic hypertension (mean 157/93 mmHg) was...

  • Theories of Endometrial Carcinogenesis: A Multidisciplinary Approach. Sherman, Mark E. // Modern Pathology;Mar2000, Vol. 13 Issue 3, p295 

    Historical observations have suggested that endometrial carcinomas vary in histopathologic appearance and clinical features. More recent, systematic studies have provided epidemiologic, clinicopathologic, and molecular support for these observations. Specifically, studies suggest that the most...

  • Problems in the Differential Diagnosis of Endometrial Hyperplasia and Carcinoma. Silverberg, Steven G. // Modern Pathology;Mar2000, Vol. 13 Issue 3, p309 

    The differential diagnosis of endometrial hyperplasia and well-differentiated endometrioid adenocarcinoma is complicated not only by the resemblance of these lesions to each other, but also by their tendency to be overdiagnosed (particularly hyperplasia) on the background of polyps,...

  • Comparative immunohistochemical study of normal, hyperplastic and neoplastic C cells of the rat thyroid gland. Martín-Lacave, I.; Rojas, F.; Bernabé, R.; Utrilla, J. C.; Fernández-Santos, J. M.; de Miguel, M.; Conde, E. // Cell & Tissue Research;Sep2002, Vol. 309 Issue 3, p361 

    In rats, the frequency of spontaneous C-cell tumours is very high and is both age and gender dependent. The three specific stages of neoplastic progression can be distinguished into diffuse C-cell hyperplasia, focal C-cell hyperplasia and bona fide C-cell tumours. Based on this hypothetical...

  • Has Cox-2 a prognostic role in non-small-cell lung cancer? A systematic review of the literature with meta-analysis of the survival results. Mascaux, C.; Martin, B.; Paesmans, M.; Berghmans, T.; Dusart, M.; Haller, A.; Lothaire, P.; Meert, A.-P.; Lafitte, J.-J.; Sculier, J.-P. // British Journal of Cancer;7/17/2006, Vol. 95 Issue 2, p139 

    Cyclooxygenase-2 (COX-2) is overexpressed in lung cancer, especially in adenocarcinoma (ADC). Our aim was to determine the prognostic value of COX-2 on survival in patients with lung cancer. Studies evaluating the survival impact of COX-2 in lung cancer, published until December 2005, were...

  • A Subset of Lung Adenocarcinomas and Atypical Adenomatous Hyperplasia-Associated Foci Are Genotypically Related: An EGFR, HER2, and K-ras Mutational Analysis. Giuliana Sartori; Alberto Cavazza; Federica Bertolini; Lucia Longo; Alessandro Marchioni; Matteo Costantini; Fausto Barbieri; Mario Migaldi; Giulio Rossi // American Journal of Clinical Pathology;Feb2008, Vol. 129 Issue 2, p202 

    Atypical adenomatous hyperplasia (AAH) is considered the preinvasive lesion of pulmonary adenocarcinoma, and mutations of EGFR, HER2, and K-ras are involved in the early stage of lung adenocarcinoma carcinogenesis, also predicting clinical response to anti-EGFR small molecule inhibitors. We...

  • Intravascular papillary endothelial hyperplasia: report of four cases in the hand. Berenguer, B.; de Salamanca Celada, J. Enríquez; González, B.; Rodríguez, P.; González, I. // European Journal of Plastic Surgery;Oct2002, Vol. 25 Issue 5, p280 

    Soft tissue tumors in the upper extremity can present challenging problems. Intravascular papillary endothelial hyperplasia may be misdiagnosed as angiosarcoma by inexperienced surgeons and pathologists. Four cases of this infrequent tumor in its most frequent location, the hand, are presented....

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics