DNA barcoding for molecular identification of Demodex based on mitochondrial genes

Yang, YuanJun; Hu, Li; Zhao, YaE; Niu, DongLing; Yang, Rui; Wang, RuiLing; Lu, Zhaohui; Li, XiaoQi
December 2017
Parasitology Research;Dec2017, Vol. 116 Issue 12, p3285
Academic Journal
There has been no widely accepted DNA barcode for species identification of Demodex. In this study, we attempted to solve this issue. First, mitochondrial cox1-5′ and 12S gene fragments of Demodex folloculorum, D. brevis, D. canis, and D. caprae were amplified, cloned, and sequenced for the first time; intra/interspecific divergences were computed and phylogenetic trees were reconstructed. Then, divergence frequency distribution plots of those two gene fragments were drawn together with mtDNA cox1-middle region and 16S obtained in previous studies. Finally, their identification efficiency was evaluated by comparing barcoding gap. Results indicated that 12S had the higher identification efficiency. Specifically, for cox1-5′ region of the four Demodex species, intraspecific divergences were less than 2.0%, and interspecific divergences were 21.1-31.0%; for 12S, intraspecific divergences were less than 1.4%, and interspecific divergences were 20.8-26.9%. The phylogenetic trees demonstrated that the four Demodex species clustered separately, and divergence frequency distribution plot showed that the largest intraspecific divergence of 12S (1.4%) was less than cox1-5′ region (2.0%), cox1-middle region (3.1%), and 16S (2.8%). The barcoding gap of 12S was 19.4%, larger than cox1-5′ region (19.1%), cox1-middle region (11.3%), and 16S (13.0%); the interspecific divergence span of 12S was 6.2%, smaller than cox1-5′ region (10.0%), cox1-middle region (14.1%), and 16S (11.4%). Moreover, 12S has a moderate length (517 bp) for sequencing at once. Therefore, we proposed mtDNA 12S was more suitable than cox1 and 16S to be a DNA barcode for classification and identification of Demodex at lower category level.


Related Articles

  • Caribbean genes. CONGER, KRISTA // Stanford Medicine;Spring2014, Vol. 31 Issue 1, p4 

    The article offers information on a research on Caribbean genes where researchers compared genetic variants in 251 people of Caribbean descent with those in more than 3,000 non-Caribbean using a new way of analyzing DNA to infer genetic ancestry.

  • Polymorphisms in the fetal progesterone receptor and a calcium-activated potassium channel isoform are associated with preterm birth in an Argentinian population. Mann, P C; Cooper, M E; Ryckman, K K; Comas, B; Gili, J; Crumley, S; Bream, E N A; Byers, H M; Piester, T; Schaefer, A; Christine, P J; Lawrence, A; Schaa, K L; Kelsey, K J P; Berends, S K; Momany, A M; Gadow, E; Cosentino, V; Castilla, E E; López Camelo, J // Journal of Perinatology;May2013, Vol. 33 Issue 5, p336 

    Objective:To investigate genetic etiologies of preterm birth (PTB) in Argentina through evaluation of single-nucleotide polymorphisms (SNPs) in candidate genes and population genetic admixture.Study Design:Genotyping was performed in 389 families. Maternal, paternal and fetal effects were...

  • Detecting very low allele fraction variants using targeted DNA sequencing and a novel molecular barcode-aware variant caller. Chang Xu; Mohammad R. Nezami Ranjbar; Zhong Wu; John DiCarlo; Yexun Wang // BMC Genomics;1/3/2017, Vol. 18, p1 

    Background: Detection of DNA mutations at very low allele fractions with high accuracy will significantly improve the effectiveness of precision medicine for cancer patients. To achieve this goal through next generation sequencing, researchers need a detection method that 1) captures rare...

  • In Vivo Interaction Between Mitochondria Carrying mtDNAs From Different Mouse Species. Sato, Akitsugu; Nakada, Kazuto; Shitara, Hiroshi; Yonekawa, Hiromichi; Hayashi, Jun-Ichi // Genetics;Aug2004, Vol. 167 Issue 4, p1855 

    Mitochondrial disease model mice, mitomice, were created using zygotes of B6mtspr strain mice carrying mitochondrial DNA (mtDNA) from Mus spretus as recipients of exogenous mitochondria carrying wild-type and a deletion mutant mtDNA (ΔmtDNA) of M. musculus domesticus. In these experiments,...

  • SUCLA2 mutations are associated with mild methylmalonic aciduria, Leigh-like encephalomyopathy, dystonia and deafness. Rosalba Carrozzo; Carlo Dionisi-Vici; Ulrike Steuerwald; Simona Lucioli; Federica Deodato; Sivia Di Giandomenico; Enrico Bertini; Barbara Franke; Leo A. J. Kluijtmans; Maria Chiara Meschini; Cristiano Rizzo; Fiorella Piemonte; Richard Rodenburg; René Santer; Filippo M. Santorelli; Arno van Rooij; Diana Vermunt-de Koning; Eva Morava; Ron A. Wevers // Brain: A Journal of Neurology;Mar2007, Vol. 130 Issue 3, p862 

    One pedigree with four patients has been recently described with mitochondrial DNA depletion and mutation in SUCLA2 gene leading to succinyl-CoA synthase deficiency. Patients had a Leigh-like encephalomyopathy and deafness but besides the presence of lactic acidosis, the profile of urine organic...

  • A Return to Linnaeus's Focus on Diagnosis, Not Description: The Use of DNA Characters in the Formal Naming of Species. RENNER, SUSANNE S. // Systematic Biology;Nov2016, Vol. 65 Issue 6, p1085 

    Descriptions and diagnoses are alternative choices in all Codes of Nomenclature because Linnaeus relied on diagnoses, not descriptions, to name ca. 13,400 animals, plants, and fungi. A diagnosis names characters in which a new taxon differs from the most similar known taxon; a description mixes...

  • Uzbekistan plans to test kids. Investor's Business Daily // Investors Business Daily;2/11/2014, pA02 

    Uzbekistan plans to test kids' genes next year to find future Olympic athletes. The tests come after the country studied genes of its top athletes for 2 years. The scientists said DNA testing would let children know what sport they would excel at. But other experts are skeptical and say that the...

  • VisualTE: a graphical interface for transposable element analysis at the genomic scale. Tempel, Sébastien; Talla, Emmanuel // BMC Genomics;2015, Vol. 16 Issue 1, p1 

    Background: Transposable elements are mobile DNA repeat sequences, known to have high impact on genes, genome structure and evolution. This has stimulated broad interest in the detailed biological studies of transposable elements. Hence, we have developed an easy-to-use tool for the comparative...

  • LOST WORLD UNDER ANTARCTICA. Brahic, Catherine // New Scientist;12/21/2013, Vol. 220 Issue 2948, p27 

    The article reports that in 2014 researchers should be able to determine the origin of DNA samples obtained in 2012 by Russian researchers from Lake Vostok in Antarctica.

  • Supplementary Materials.  // Cancers;Dec2013, Vol. 5 Issue 4, pS1 

    The article presents a supplementary materials used in the 2013 issue of the journal "Cancers" such as a figure regarding promoter structure for methylation analysis of Ras association domain family members (RASSF) RASSF2, RASSF5A, RASSF5C, and RASSF10, a table on primer and polymerase chain...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics