TITLE

Molecular and immunological characterisation of tropomyosin from Anisakis pegreffii

AUTHOR(S)
Asnoussi, Abdouslam; Aibinu, Ibukun; Smooker, Peter; Gasser, Robin; Lopata, Andreas
PUB. DATE
December 2017
SOURCE
Parasitology Research;Dec2017, Vol. 116 Issue 12, p3291
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Tropomyosin (TM) is a major allergen in shellfish, known to cross-react with mite, cockroach and/or some roundworm (nematode) TM. In this study, we aimed to express and purify TM from the parasitic nematode Anisakis pegreffii and also to characterise its cross-reactivity with TM from shellfish. A. pegreffii was isolated from the flathead tiger fish ( Neoplatycephalus richardsoni) and characterised using single-strand conformation polymorphism (SSCP)-based sequencing of the first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA. The recombinant tropomyosin (rTM) of A. pegreffii was expressed, purified and confirmed by immunohistochemistry, sequencing and LC-MS/MS analyses. Immunohistochemistry showed the muscle and the base layer of the third-stage larvae (L3) of A. pegreffii as the location of TM in A. pegreffii. The molecular relationship of TM of A. pegreffii with homologs from other nematodes and crustaceans was inferred from phylogenetic analysis. Immunogenicity of TM from A. pegreffii was tested by immunoblotting, which showed that rTM from A. pegreffii binds to IgE from sera of patients with allergy to crustaceans. Immunoblotting also showed that the anti-TM monoclonal antibody (MAb) did not recognise rTM from A. pegreffii. The rTM from A. pegreffii was, however, recognised by anti-TM polyclonal antibodies (PAbs) as well as anti-crustacean polyclonal antibodies (PAbs). The detection of specific serum IgE antibody against parasite TM has been proposed as a useful approach for the diagnosis of parasite-induced allergy. The findings of this study merit further exploration of the cross-reactive allergenic proteins of Anisakis for improved, future diagnosis of allergenic diseases.
ACCESSION #
126260342

 

Related Articles

  • Identification of tropomyosin as a major allergen in the octopus Octopus vulgaris and elucidation of its IgE-binding epitopes. Ishikawa, Masaru; Suzuki, Fumi; Ishida, Masami; Nagashima, Yuji; Shiomi, Kazuo // Fisheries Science;Oct2001, Vol. 67 Issue 5, p934 

    The major allergen (named Oct v 1) in the muscle of the octopus Octopus vulgaris was purified by gel filtration on Sephacryl S-300, anion-exchange fast protein liquid chromatography on Mono Q and reverse-phase high-pressure liquid chromatography on TSKgel Octadecyl-4PW. In addition to the...

  • Tropomyosin, the major tropical oyster Crassostrea belcheri allergen and effect of cooking on its allergenicity. Mohamad Yadzir, Zailatul Hani; Misnan, Rosmilah; Bakhtiar, Faizal; Abdullah, Noormalin; Murad, Shahnaz // Allergy, Asthma & Clinical Immunology;10/26/2015, Vol. 11, p1 

    Background: Many types of shellfish including oysters are sometime cooked before ingestion and it has been demonstrated that cooking may affect the allergenicity of food. Therefore, the aim of our present study is to identify major and minor allergens of tropical oyster (Crassostrea belcheri)...

  • Molecular cloning and expression of two new allergens from Anisakis simplex. Yukihiro Kobayashi; Shoichiro Ishizaki; Kuniyoshi Shimakura; Yuji Nagashima; Kazuo Shiomi // Parasitology Research;May2007, Vol. 100 Issue 6, p1233 

    Abstract  The nematodeAnisakis simplexis a marine parasite that causes allergy as well as anisakiasis. Although fiveAnisakisallergens have already been identified, immunoblotting studies suggested that unidentified allergens still exist. In this study, an expression cDNA library...

  • In Vitro Gastric and Intestinal Digestions of Pulsed Light-Treated Shrimp Extracts. Yang, Wade; Shriver, Sandra; Chung, Si-yin; Percival, Susan; Correll, Melanie; Rababah, Taha // Applied Biochemistry & Biotechnology;Apr2012, Vol. 166 Issue 6, p1409 

    Pulsed ultraviolet light (PUV), a novel technology most commonly used for microbial inactivation, has recently been employed to effectively mitigate food allergens in peanuts, soybean, shrimp, and almond. Putative mechanisms for the efficacy of PUV in reducing allergen reactivity include...

  • Helminth allergens, parasite-specific IgE, and its protective role in human immunity. Fitzsimmons, Colin Matthew; Falcone, Franco Harald; Dunne, David William // Frontiers in Immunology;Feb2014, Vol. 5, p1 

    The Th2 immune response, culminating in eosinophilia and IgE production, is not only characteristic of allergy but also of infection by parasitic worms (helminths). Anti-parasite IgE has been associated with immunity against a range of helminth infections and many believe that IgE and its...

  • Synergism between Prior Anisakis simplex Infections and Intake of NSAIDs, on the Risk of Upper Digestive Bleeding: A Case-Control Study. Ubeira, Florencio M.; Anadón, Ana M.; Salgado, Angel; Carvajal, Alfonso; Ortega, Sara; Aguirre, Carmelo; López-Goikoetxea, María José; Ibanez, Luisa; Figueiras, Adolfo // PLoS Neglected Tropical Diseases;Jun2011, Vol. 5 Issue 6, p1 

    Background: The aim of this study was to investigate the relationship between prior Anisakis infections and upper gastrointestinal bleeding (UGIB), and its interaction with non-steroidal anti-inflammatory drug (NSAID) intake. Methods/Principal Findings: We conducted a hospital-based case-control...

  • Simultaneous Detection and Prevalence of Allergens in Anisakis Species Isolated from Marine Fishes. WOO JOO LEE; DONG JOO SEO; HYEJIN OH; SU BEEN JEON; DAY JUNG; CHANGSUN CHOI // Journal of Food Protection;May2016, Vol. 79 Issue 5, p789 

    This study was conducted to develop a multiplex reverse transcription (RT) PCR for the detection of Anisakis allergens and to investigate the relationship between allergen profiles and anisakid larvae isolated from Scomber japonicus, Trichiurus lepturus, and Conger myriaster in Korea. The...

  • Allergenic Tropomyosins and Their Cross-Reactivities. Kyoung Yong Jeong; Chein-Soo Hong; Tai-Soon Yong // Protein & Peptide Letters;Aug2006, Vol. 13 Issue 8, p835 

    The ingestion or inhalation of some proteins may lead to adverse immune reactions. Allergens may trigger allergic reactions in genetically predisposed individuals when they are absorbed through the skin or make contact with mucous membranes. An allergic disease often deteriorates the quality of...

  • Evaluation of In Silico Prediction Possibility of Epitope Sequences Using Experimental Data Concerning Allergenic Food Proteins Summarised in BIOPEP Database. Minkiewicz, Piotr; Dziuba, Jerzy; Darewicz, Małgorzata; Bucholska, Justyna; Mogut, Damir // Polish Journal of Food & Nutrition Sciences;2012, Vol. 62 Issue 3, p151 

    The aim of the study was to evaluate the possibility of predicting potential epitope sequences and location in allergenic proteins from food using EVALLER program by comparison with experimental epitopes summarised in the BIOPEP database of allergenic proteins. Sequences of experimental epitopes...

  • Molecular Cloning and the Allergenic Characterization of Tropomyosin from Tyrophagus putrescentiae. Kyoung Yong Jeong; Haeseok Lee; Jae Sik Lee; Jongweon Lee; In-Yong Lee; Han-Il Ree; Chein-Soo Hong; Tai-Soon Yong // Protein & Peptide Letters;May2007, Vol. 14 Issue 5, p431 

    Storage mites have been recognized as a cause of asthma and rhinitis. Studies from several countries have shown that the IgE-mediated allergy to storage mites is of considerable importance, especially in rural populations. This study aimed to identify and characterize new allergens from...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics