Natural Plasmodium vivax infections in Anopheles mosquitoes in a malaria endemic area of northeastern Thailand

Poolphol, Petchaboon; Harbach, Ralph; Sriwichai, Patchara; Aupalee, Kittipat; Saeung, Atiporn; Chaithong, Udom; Sattabongkot, Jetsumon; Kumpitak, Chalermpon; Srisuka, Wichai; Taai, Kritsana; Thongsahuan, Sorawat; Phuackchantuck, Rochana
December 2017
Parasitology Research;Dec2017, Vol. 116 Issue 12, p3349
Academic Journal
There was recently an outbreak of malaria in Ubon Ratchathani Province, northeastern Thailand. In the absence of information on malaria vector transmission dynamics, this study aimed to identify the anopheline vectors and their role in malaria transmission. Adult female Anopheles mosquitoes were collected monthly by human-landing catch in Na Chaluai District of Ubon Ratchathani Province during January 2014−December 2015. Field-captured mosquitoes were identified to species using morphology-based keys and molecular assays (allele-specific polymerase chain reaction, AS-PCR), and analysed for the presence of Plasmodium falciparum and Plasmodium vivax using an enzyme-linked immunosorbent assay (ELISA) for the detection of circumsporozoite proteins (CSP). A total of 1,229 Anopheles females belonging to 13 species were collected. Four anopheline taxa were most abundant: Members of the Anopheles barbirostris complex, comprising 38% of the specimens, species of the Anopheles hyrcanus group (18%), Anopheles nivipes (17%) and Anopheles philippinensis (12%). The other nine species comprised 15% of the collections. Plasmodium infections were detected in two of 668 pooled samples of heads/thoraces, Anopheles dirus (1/29) and An. philippinensis (1/97). The An. dirus pool had a mixed infection of P. vivax-210 and P. vivax-247, whereas the An. philippinensis pool was positive only for the latter protein variant. Both positive ELISA samples were confirmed by nested PCR. This study is the first to incriminate An. dirus and An. philippinensis as natural malaria vectors in the area where the outbreak occurred. This information can assist in designing and implementing a more effective malaria control programme in the province.


Related Articles

  • Biting behaviour of African malaria vectors: 1. where do the main vector species bite on the human body? Braack, Leo; Hunt, Richard; Koekemoer, Lizette L.; Gericke, Anton; Munhenga, Givemore; Haddow, Andrew D.; Becker, Piet; Okia, Michael; Kimera, Isaac; Coetzee, Maureen // Parasites & Vectors;2015, Vol. 8 Issue 1, p1 

    Background: Malaria control in Africa relies heavily on indoor vector management, primarily indoor residual spraying and insecticide treated bed nets. Little is known about outdoor biting behaviour or even the dynamics of indoor biting and infection risk of sleeping household occupants. In this...

  • WHO calls for strengthening malaria elimination strategy, says 3.2 billion people still at risk.  // Express Healthcare;7/31/2015, p1 

    The article reports on the demand of the World Health Organization (WHO) to strengthen its strategy to eliminate and control the continuous risk of malaria globally. WHO regional director Poonam Khetrapal Singh mentions the organization's efforts on plasmodium falciparum (P falciparum) malaria...

  • Malaria and its Vectors in the Caribbean: The Continuing Challenge of the Disease Forty-Five Years after Eradication from the Islands. Rawlins, S. C.; Hinds, A.; Rawlins, J. M. // West Indian Medical Journal;Nov2008, Vol. 57 Issue 5, p462 

    Objectives: Given the occurrence of autochthonous malaria in non-endemic island countries in the last 10 years, this study evaluates the risk factors for malaria transmission in the malaria “endemic and “non-endemic” countries of the Caribbean region. Design: Data on imported...

  • Evaluation of a real-time quantitative PCR to measure the wild Plasmodium falciparum infectivity rate in salivary glands of Anopheles gambiae. Marie, Alexandra; Boissière, Anne; Tsapi, Majoline Tchioffo; Poinsignon, Anne; Awono-Ambéné, Parfait H.; Morlais, Isabelle; Remoue, Franck; Cornelie, Sylvie // Malaria Journal;2013, Vol. 12 Issue 1, p1 

    Background: Evaluation of malaria sporozoite rates in the salivary glands of Anopheles gambiae is essential for estimating the number of infective mosquitoes, and consequently, the entomological inoculation rate (EIR). EIR is a key indicator for evaluating the risk of malaria transmission....

  • How the Malaria Vector Anopheles gambiae Adapts to the Use of Insecticide-Treated Nets by African Populations. Ndiath, Mamadou Ousmane; Mazenot, Catherine; Sokhna, Cheikh; Trape, Jean-François // PLoS ONE;Jun2014, Vol. 9 Issue 6, p1 

    Background: Insecticide treated bed nets have been recommended and proven efficient as a measure to protect African populations from malaria mosquito vector Anopheles spp. This study evaluates the consequences of bed nets use on vectors resistance to insecticides, their feeding behavior and...

  • STEMMING THE FLOW.  // Geographical (Geographical Magazine Ltd.);Dec2015, Vol. 87 Issue 12, p6 

    The article focuses on a study conducted in the University of New England in Australia regarding the high risk of people living near dam reservoirs in sub-Saharan Africa to suffer and contract malaria, and the spread of Anopheles mosquitoes.

  • Anopheles darlingi (Diptera: Culicidae) displays increased attractiveness to infected individuals with Plasmodium vivax gametocytes. Batista, Elis P. A.; Costa, Elizangela F. M.; Silva, Alexandre A. // Parasites & Vectors;2014, Vol. 7 Issue 1, p1 

    Background Most hematophagous insects use host odours as chemical cues. The odour components, some physiological parameters and host attractiveness are affected by several conditions, including infection by parasites, e.g., plasmodia and, therefore, change the epidemiological scenario. This...

  • No Difference in the Incidence of Malaria in Human-Landing Mosquito Catch Collectors and Non-Collectors in a Senegalese Village with Endemic Malaria. Wotodjo, Amélé N.; Trape, Jean-François; Richard, Vincent; Doucouré, Souleymane; Diagne, Nafissatou; Tall, Adama; Ndiath, Ousmane; Faye, Ngor; Gaudart, Jean; Rogier, Christophe; Sokhna, Cheikh // PLoS ONE;May2015, Vol. 10 Issue 5, p1 

    Background: The human landing catches is the gold standard method used to study the vectors of malaria and to estimate their aggressiveness. However, this method has raised safety concerns due to a possible increased risk of malaria or other mosquito-borne diseases among the mosquito...

  • Epidemiological Profile of Malaria: Analysis in District Amritsar. Mahajan, S. L. // Indian Journal of Public Health Research & Development;Jul-Sep2016, Vol. 7 Issue 3, p215 

    Introduction: Malaria is a mosquito-borne illness. In 2014, 97 countries and territories had ongoing malaria transmission. An estimated 3.3 billion people are at risk of malaria. It is a leading cause of death for children. The Anopheles mosquito transmits malaria parasites to humans, thrives in...

  • Men Traveling Away from Home Are More Likely to Bring Malaria into High Altitude Villages, Northwest Ethiopia. Alemu, Kassahun; Worku, Alemayehu; Berhane, Yemane; Kumie, Abera // PLoS ONE;Apr2014, Vol. 9 Issue 4, p1 

    Background: Information about malaria risk factors at high altitudes is scanty. Understanding the risk factors that determine the risk of malaria transmission at high altitude villages is important to facilitate implementing sustainable malaria control and prevention programs. Methods: An...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics