TITLE

Immunogenic properties of the human gut-associated archaeon Methanomassiliicoccus luminyensis and its susceptibility to antimicrobial peptides

AUTHOR(S)
Bang, Corinna; Vierbuchen, Tim; Gutsmann, Thomas; Heine, Holger; Schmitz, Ruth A.
PUB. DATE
October 2017
SOURCE
PLoS ONE;10/05/2017, Vol. 12 Issue 10, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The methanogenic archaeon Methanomassiliicoccus luminyensis strain B10T was isolated from human feces just a few years ago. Due to its remarkable metabolic properties, particularly the degradation of trimethylamines, this strain was supposed to be used as “Archaebiotic” during metabolic disorders of the human intestine. However, there is still no data published regarding adaptations to the natural habitat of M. luminyensis as it has been shown for the other two reported mucosa-associated methanoarchaea. This study aimed at unraveling susceptibility of M. luminyensis to antimicrobial peptides as well as its immunogenicity. By using the established microtiter plate assay adapted to the anaerobic growth requirements of methanogenic archaea, we demonstrated that M. luminyensis is highly sensitive against LL32, a derivative of human cathelicidin (MIC = 2 μM). However, the strain was highly resistant against the porcine lysin NK-2 (MIC = 10 μM) and the synthetic antilipopolysaccharide peptide (Lpep) (MIC>10 μM) and overall differed from the two other methanoarchaea, Methanobrevibacter smithii and Methanosphaera stadtmanae in respect to AMP sensitivity. Moreover, only weak immunogenic potential of M. luminyensis was demonstrated using peripheral blood mononuclear cells (PBMCs) and monocyte-derived dendritic cells (moDCs) by determining release of pro-inflammatory cytokines. Overall, our findings clearly demonstrate that the archaeal gut inhabitant M. luminyensis is susceptible to the release of human-derived antimicrobial peptides and exhibits low immunogenicity towards human immune cells in vitro–revealing characteristics of a typical commensal gut microbe.
ACCESSION #
125495838

 

Related Articles

  • Antimicrobial implications of vitamin D. Youssef, Dima A.; Miller, Christopher W.T.; El-Abbassi, Adel M.; Cutchins, Della C.; Cutchins, Coleman; Grant, William B.; Peiris, Alan N. // Dermato-Endocrinology;2011, Vol. 3 Issue 4, Special section p1 

    Evidence exists that vitamin D has a potential antimicrobial activity and its deficiency has deleterious effects on general well-being and longevity. Vitamin D may reduce the risk of infection through multiple mechanisms. Vitamin D boosts innate immunity by modulating production of...

  • Toll-like receptors in the skin. Miller, Lloyd; Modlin, Robert // Seminars in Immunopathology;2007, Vol. 29 Issue 1, p15 

    Toll-like receptors (TLRs) are important pattern-recognition receptors involved in host defense against a variety of pathogenic microorganisms. Activation of TLRs leads to the production of cytokines, chemokines, antimicrobial peptides, and upregulation costimulatory and adhesion molecules...

  • The Imd Pathway Is Involved in Antiviral Immune Responses in Drosophila. Costa, Alexandre; Jan, Eric; Sarnow, Peter; Schneider, David // PLoS ONE;2009, Vol. 4 Issue 10, p1 

    Cricket Paralysis virus (CrPV) is a member of the Dicistroviridae family of RNA viruses, which infect a broad range of insect hosts, including the fruit fly Drosophila melanogaster. Drosophila has emerged as an effective system for studying innate immunity because of its powerful genetic...

  • The role of immune system in the development of periodontal disease: a brief review. Mariano, Flávia Sammartino; de Cássia Orlandi Sardi, Janaina; Duque, Cristiane; Höfling, José Francisco; Gonçalves, Reginaldo Bruno // Revista Odonto Ciencia;2010, Vol. 25 Issue 3, p300 

    Periodontitis is a highly complex and multi-factorial disease. This review summarizes some immunological factors involved in the development and control of this oral disease, such as: the participation of inflammatory cells in local inflammation, the synthesis of chemotaxis proteins with...

  • Flora: Role in Colonisation Resistance and Other Effects; Production of Antimicrobial Peptides. Axelsson, Lars-Göran; Mahida, Yashwant // Microbial Ecology in Health & Disease;Dec2000 Supplement 2, Vol. 12, p216 

    This communication describes the innate immunity, activated upon exposure to foreign antigens without prior priming, and the role of antimicrobial peptides in this system. The focus will be on peptides produced by gastrointestinal cells (Paneth cells). Possible applications for the...

  • Immune system and Gut Flora Interactions Are Important Episodes in metabolic Diseases. Jain, Shalini; Marotta, Francesco; Catanzaro, Roberto; Yadav, Hariom // Journal of Gastrointestinal & Liver Diseases;Dec2012, Vol. 21 Issue 4, p347 

    The authors reflect on gut-flora-immune interactions as significant episodes in metabolic diseases. They say that metabolic diseases, such as cardiovascular diseases (CVDs), obesity, and diabetes, are linked with low grade chronic inflammation and inflammatory state is showed with increased...

  • A Fourier Transformation based Method to Mine Peptide Space for Antimicrobial Activity. Nagarajan, Vijayaraj; Kaushik, Navodit; Murali, Beddhu; Chaoyang Zhang; Lakhera, Sanyogita; Elasri, Mohamed O.; Youping Deng // BMC Bioinformatics;2006 Supplement 2, Vol. 7, pS2 

    Background: Naturally occurring antimicrobial peptides are currently being explored as potential candidate peptide drugs. Since antimicrobial peptides are part of the innate immune system of every living organism, it is possible to discover new candidate peptides using the available genomic and...

  • Cytokine responses of intraepithelial lymphocytes are regulated by histamine H2 receptor. Takagaki, Kosuke; Osawa, Satoshi; Horio, Yoshiaki; Yamada, Takanori; Hamaya, Yasushi; Takayanagi, Yasuhiro; Furuta, Takahisa; Hishida, Akira; Ikuma, Mutsuhiro // Journal of Gastroenterology;2009, Vol. 44 Issue 4, p285 

    Histamine participates in the immune regulation of several gastrointestinal diseases. However, the effect of histamine on intestinal intraepithelial lymphocytes (IELs), the front line of the intestinal mucosal immune system, is not well understood. We examined whether histamine has a direct...

  • Gastrointestinal Immune System and Brain Dialogue Implicated in Neuroinflammatory and Neurodegenerative Diseases. Deretzi, G.; Kountouras, J.; Polyzos, S. A.; Zavos, C.; Giartza-Taxidou, E.; Gavalas, E.; Tsiptsios, I. // Current Molecular Medicine;Nov2011, Vol. 11 Issue 8, p696 

    A common characteristic of the central nervous system (CNS) neurodegenerative disorders is neuroinflammation, marked by augmented numbers of activated and primed microglia, increased inflammatory cytokines and decreased anti-inflammatory molecules. CNS neuroinflammation is a critical component...

  • Immunonutrition with long-chain fatty acids prevents activation of macrophages in the gut wall. Eisner, Friederike; Jacob, Petra; Frick, Julia-Stefanie; Feilitzsch, Maximilian; Geisel, Julia; Mueller, Mario; Küper, Markus; Raybould, Helen; Königsrainer, Ingmar; Glatzle, Jörg; Mueller, Mario H; Küper, Markus A; Raybould, Helen E; Königsrainer, Ingmar; Glatzle, Jörg // Journal of Gastrointestinal Surgery;May2011, Vol. 15 Issue 5, p853 

    Background: Immune cells and inflammatory mediators are released from the gastrointestinal tract into the mesenteric lymph during sepsis causing distant organ dysfunction. Recently, it was demonstrated that macrophages in the gut wall are controlled by the vagus nerve, the so-called...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics