TGFβ-1 and TNFα Expression in the Epidermis of Patients with Epidermodysplasia Verruciformis

Majewski, Slawomir; Hunzelmann, Nicolas; Nischt, Roswitha; Eckes, Beate; Rudnicka, Lidia; Orth, Gerard; Krieg, Thomas; Jablonska, Stefania
November 1991
Journal of Investigative Dermatology;Nov91, Vol. 97 Issue 5, p862
Academic Journal
In epidermodysplasia verruciformis (EV), the infection with specific human papillomaviruses (HPV) might be under control of the local immunosurveillance mechanisms related to cytokines produced by epidermal cells. We have investigated by in situ hybridization the expression of mRNA coding for TGFβ-1 and TNFα in the skin of patients with EV (n = 4) as compared to the skin lesions of patients with other premalignant (actinic keratosis; n = 5) or malignant (squamous cell carcinoma; n = 4) skin lesions, and to the skin of healthy individuals (n = 5). The expression of TGFβ-1 and TNFα mRNA was higher in the epidermis of EV patients as compared to the control skin from healthy individuals. The increased expression of mRNA for both cytokines was confirmed by northern blot analysis of RNA isolated from the skin lesions of the patient with EV. No specific signals for TGFβ-1 and TNFα were detected in actinic keratosis, and in cases of squamous cell carcinomas only single neoplastic cells were positive for TGFβ-1. It is conceivable that in EV TGFβ-1 and TNFα can be involved in the regulation of the growth and differentiation of HPV-infected keratinocytes and in the persistence of HPV-induced skin lesions.


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