TITLE

Surfactants Cause In Vitro Chemotaxis and Chemokinesis of Human Neutrophils

AUTHOR(S)
Frosch, Peter J.; Czarnetzki, Beate M.
PUB. DATE
March 1987
SOURCE
Journal of Investigative Dermatology;Mar87 Supplement, Vol. 88, p52s
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The surfactant sodium lauryl sulfate (SLS) and alkyldimethylbenzylammonium chloride (ADB) cause erythema and leukocyte infiltration on epicutaneous application. To elucidate the mechanism of this inflammatory response, the in vitro effect of the same agents was studied on human neutrophil migration, basophil histamine release, and leukocyte lysosomal enzyme (β-glucuronidase) release. At concentrations of greater than 0.02%, both surfactants were cytotoxic, as was evident by decreased cosin exclusion, massive histamine and β-g1ucuronidase--release, and absent migration of cells. At dilutions of less than 0.002% of both surfactants, viability of cells was normal, and small amounts of histamine and β-glucuronidase were released at a dilution of 0.001 %. The most striking finding was a dose-dependent chemotactic and chemokinetic response at dilutions from 10-3 to 10-8% These observations offer a possible explanation for the pathomechanisms of irritant dermatitis due to surfactants.
ACCESSION #
12468943

 

Related Articles

  • ASSESSMENT OF THE PHAGOCYTIC FUNCTION AND OXIDATIVE BURST IN BLOOD LEUKOCYTES BY FLOW CYTOMETRY. Sibinovska, Olgica; Trajkov, Dejan; Petlichkovski, Aleksandar; Strezova, Ana; Efinska-Mladenovska, Olivija; Hristomanova, Slavica; Djulejic, Eli; Petrov, Jordan; Spiroski, Mirko // Macedonian Journal of Medical Sciences;2008 Supplement 1, Vol. 1, pS47 

    Phagocytosis by polymorphonuclear neutrophils and monocytes constitutes an essential arm of the first-line defense against bacterial or fungal infections. The phagocytic process consists of several subsequent steps such as: chemotaxis, attachment of particles to the cell surface of phagocytes,...

  • CHEMOTAXIS OF HUMAN BLOOD MONOCYTES STUDIED USING A UNIQUE 8μm "SPARSE-PORE" POLYCARBONATE MEMBRANE IN MICRO-BOYDEN CHAMBERS.  // Immunology & Cell Biology;Dec1993, Vol. 71 Issue 6, special section pS20 

    The article presents an abstract of the research paper "Chemotaxis of Human Blood Monocytes Studied Using a Unique 8µm "Sparse-Pore" Porepolycarbonate Membrane in Micro-Boyden Chambers," by D.G. Harkin and L.P. Bignold. Monocyte chemotaxis in vitro has been investigated using the same general...

  • Evaluation of human leukocyte N-formylpeptide receptor (FPR1) SNPs in aggressive periodontitis patients. Zhang, Y; Syed, R; Uygar, C; Pallos, D; Gorry, M C; Firatli, E; Cortelli, J R; VanDyke, T E; Hart, P S; Feingold, E; Hart, T C // Genes & Immunity;Jan2003, Vol. 4 Issue 1, p22 

    Polymorphonuclear neutrophils (PMNs) are attracted to sites of infection by N-formylpeptide (fMLP) chemoattractants. The high-affinity fMLP receptor (FPR1) of phagocytic cells interacts with bacterial fMLP and mediates chemotaxis, degranulation, and superoxide production. These cellular...

  • PI(3)Kγ has an important context-dependent role in neutrophil chemokinesis. Ferguson, G. John; Milne, Laura; Kulkarni, Suhasini; Sasaki, Takehiko; Walker, Simon; Andrews, Simon; Crabbe, Tom; Finan, Peter; Jones, Gareth; Jackson, Shaun; Camps, Montserrat; Rommel, Christian; Wymann, Matthias; Hirsch, Emilio; Hawkins, Phillip; Stephens, Len // Nature Cell Biology;Jan2007, Vol. 9 Issue 1, p86 

    The directional movement of cells in a gradient of external stimulus is termed chemotaxis and is important in many aspects of development and differentiated cell function. Phophoinositide 3-kinases (PI(3)Ks) are thought to have critical roles within the gradient-sensing machinery of a variety of...

  • High Fidelity Processing and Activation of the Human a- Defensin HNP1 Precursor by Neutrophil Elastase and Proteinase 3. Tongaonkar, Prasad; Golji, Amir E.; Tran, Patti; Ouellette, AndréJ.; Selsted, Michael E. // PLoS ONE;Mar2012, Vol. 7 Issue 3, p1 

    The azurophilic granules of human neutrophils contain four a-defensins called human neutrophil peptides (HNPs 1-4). HNPs are tridisulfide-linked antimicrobial peptides involved in the intracellular killing of organisms phagocytosed by neutrophils. The peptides are produced as inactive precursors...

  • neutrophil:. Huber, Jeffrey T.; Gillaspy, Mary L. // Encyclopedic Dictionary of AIDS-Related Terminology;2000, p161 

    This article presents an encyclopedia entry for "neutrophil." The term refers to a granular leukocyte with the properties of adherence to immune complexes, chemotaxis and phagocytosis. They are readily stainable with neutral dyes. The term also refers to any cell, structure, or histologic...

  • neutrophil, neutrophile.  // Taber's Cyclopedic Medical Dictionary;2005, p1465 

    A definition of the term "neutrophil or neutrophile" is presented. It refers to granular white blood cell (WBC), which is responsible for body's protection against infection. Neutrophils destroy foreign antigens (chemotaxis) through phagocytosis. Inadequate number of neutrophils renders the body...

  • Leukocyte Subset Changes in Response to a 164-km Road Cycle Ride in a Hot Environment. HUI-YING LUK; MCKENZIE, AMY L.; DUPLANTY, ANTHONY A.; BUDNAR, RONALD G.; LEVITT, DANIELLE; FERNANDEZ, ALEX; LEE, ELAINE C.; ARMSTRONG, LAWRENCE E.; VINGREN, JAKOB L. // International Journal of Exercise Science;2016, Vol. 9 Issue 1, p34 

    The purpose of this observational study was to determine the circulating leukocyte subset response to completing the 2013 Hotter'N Hell Hundred recreational 164-km road cycle event in a hot and humid environmental condition. Twenty-eight men and four women were included in this study. Whole...

  • Neutrophil Studies in Psoriatics: In Vivo Migration, Phagocytosis and Bacterial Killing. Dubertret, L.; Lebreton, C.; Touraine, H. // Journal of Investigative Dermatology;Aug82, Vol. 79 Issue 2, p74 

    A reproducible method for sequential study of migration out of human skin, phagocytosis and bactericidal activity of neutrophils is described. Untreated psoriatics exhibit an early increase of chemotactic activity (0-8 hr, p < 0.02) and subsequently a strong inhibition of chemotaxis...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics