Tyrosinase Gene Transcription and Its Control by Melanogenic Inhibitors

Ando, Shunsaku; Ando, Osamu; Suemoto, Yasuo; Mishima, Yutaka
February 1993
Journal of Investigative Dermatology;Feb93 Supplement, Vol. 100, p150S
Academic Journal
The levels of tyrosinase mRNA and tyrosinase activity were analyzed in two amelanotic melanoma cell lines, D1 178 (hamster) and G-361 (human). Neither tyrosinase mRNA nor tyrosinase activity were detected in D1 178 cells. On the other hand, both tyrosinase mRNA and weak tyrosinase activity were detected in G-361 cells. Assuming that the different types of melanogenic inhibitors affected melanogenesis in these two amelanotic melanoma cells in different manners, we performed a screening of melanogenic inhibitors in these two cell lines. As an isolated tyrosinase suppressive melanogenic inhibitor, ascorbic acid and glutathione were identified from D1 178 cells and G-361 cells, respectively. Furthermore, lactic acid was identified from D1 178 cells as an isolated tyrosinase non-suppressive melanogenic inhibitor. B-16 mouse melanotic melanoma cells were depigmented by treatment with lactic acid. The melanogenesis suppression by lactic acid in B-16 cells was found to be due to inhibition of tyrosinase gene expression.


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