TITLE

Tyrosinase Gene Transcription and Its Control by Melanogenic Inhibitors

AUTHOR(S)
Ando, Shunsaku; Ando, Osamu; Suemoto, Yasuo; Mishima, Yutaka
PUB. DATE
February 1993
SOURCE
Journal of Investigative Dermatology;Feb93 Supplement, Vol. 100, p150S
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The levels of tyrosinase mRNA and tyrosinase activity were analyzed in two amelanotic melanoma cell lines, D1 178 (hamster) and G-361 (human). Neither tyrosinase mRNA nor tyrosinase activity were detected in D1 178 cells. On the other hand, both tyrosinase mRNA and weak tyrosinase activity were detected in G-361 cells. Assuming that the different types of melanogenic inhibitors affected melanogenesis in these two amelanotic melanoma cells in different manners, we performed a screening of melanogenic inhibitors in these two cell lines. As an isolated tyrosinase suppressive melanogenic inhibitor, ascorbic acid and glutathione were identified from D1 178 cells and G-361 cells, respectively. Furthermore, lactic acid was identified from D1 178 cells as an isolated tyrosinase non-suppressive melanogenic inhibitor. B-16 mouse melanotic melanoma cells were depigmented by treatment with lactic acid. The melanogenesis suppression by lactic acid in B-16 cells was found to be due to inhibition of tyrosinase gene expression.
ACCESSION #
12465056

 

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