TITLE

Probing ion channel conformational dynamics using simultaneous single-molecule ultrafast spectroscopy and patch-clamp electric recording

AUTHOR(S)
Harms, Greg; Orr, Galya; Lu, H. Peter
PUB. DATE
March 2004
SOURCE
Applied Physics Letters;3/8/2004, Vol. 84 Issue 10, p1792
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An approach to probing single-molecule ion channel kinetics and conformational dynamics, patch-clamp confocal fluorescence microscopy (PCCFM), uses simultaneous ultrafast fluorescence spectroscopy and single-channel electric current recording. PCCFM is applied to determine single-channel conformational dynamics by probing single-pair fluorescence resonant energy transfer, fluorescence self-quenching, and anisotropy of the dye-labeled gramicidin ion channel incorporated in an artificial lipid bilayer. Hidden conformational changes were observed, which strongly suggests that multiple intermediate conformation states are involved in gramicidin ion channel dynamics. © 2004 American Institute of Physics.
ACCESSION #
12405685

 

Related Articles

  • Gating of an organic transistor through a bilayer lipid membrane with ion channels. Bernards, Daniel A.; Malliaras, George G.; Toombes, Gilman E. S.; Gruner, Sol M. // Applied Physics Letters;7/31/2006, Vol. 89 Issue 5, p053505 

    The authors use bilayer lipid membranes (BLMs) as a means to control the gating of organic electrochemical transistors (OECTs). Upon formation of a high quality BLM, the gating of an OECT can be fully suppressed. Gating is restored when gramicidin ion channels are incorporated into the BLM. The...

  • Lipid bilayer array for simultaneous recording of ion channel activities. Hirano-Iwata, Ayumi; Nasu, Tomohiro; Oshima, Azusa; Kimura, Yasuo; Niwano, Michio // Applied Physics Letters;7/9/2012, Vol. 101 Issue 2, p023702 

    This paper describes an array of stable and reduced-solvent bilayer lipid membranes (BLMs) formed in microfabricated silicon chips. BLMs were first vertically formed simultaneously and then turned 90° in order to realize a horizontal BLM array. Since the present BLMs are mechanically stable...

  • Sensing-Applications of Surface-Based Single Vesicle Arrays. Christensen, Sune M.; Stamou, Dimitrios G. // Sensors (14248220);2010, Vol. 10 Issue 12, p11352 

    A single lipid vesicle can be regarded as an autonomous ultra-miniaturised 3D biomimetic "scaffold" (Ø ≥ 13 nm) ideally suited for reconstitution and interrogation of biochemical processes. The enclosing lipid bilayer membrane of a vesicle can be applied for studying binding...

  • Investigation of Ion Channel Activities of Gramicidin A in the Presence of Ionic Liquids Using Model Cell Membranes. Ryu, Hyunil; Choi, Sangbaek; Jeon, Tae-Joon; Lee, Hwankyu; Iwata, Seigo; Maruta, Shinsaku; Ki Kim, Moon; Kim, Young-Rok; Min Kim, Sun // Scientific Reports;7/24/2015, p11935 

    Ionic liquids (ILs) are considered to be green solvents because of their non-volatility. Although ILs are relatively safe in the atmospheric environment, they may be toxic in other environments. Our previous research showed that the cytotoxicity of ILs to biological organisms is attributable to...

  • A Förster resonance energy transfer (FRET) approach for enhancing fluorescence contrast in phase-separated membranes. Carter Ramirez, Daniel M.; Ding, Jason; Guan, Jack; Vobornik, Dusan; Carnini, Anna; Ogilvie, William W.; Jakubek, Zygmunt J.; Johnston, Linda J. // Canadian Journal of Chemistry;Mar2011, Vol. 89 Issue 3, p423 

    The partitioning of the dye-labeled lipid probe, NBD-DHPE (1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt)), was examined by fluorescence microscopy in phase-separated lipid bilayers with mixtures of coexisting liquid-ordered and fluid...

  • Gating charge displacement in voltage-gated ion channels involves limited transmembrane movement. Chanda, Baron; Asamoah, Osei Kwame; Blunck, Rikard; Roux, Benoît; Bezanilla, Francisco // Nature;8/11/2005, Vol. 436 Issue 7052, p852 

    Voltage-gated ion channels are responsible for generating electrical impulses in nerves and other excitable cells. The fourth transmembrane helix (S4) in voltage-gated channels is the primary voltage-sensing unit that mediates the response to a changing membrane electric field. The molecular...

  • Gramicidin channel controversy ? revisited. Cross, T.A.; Arseniev, A.; Cornell, B.A.; Davis, J.H.; Killian, J.A.; Koeppe, R.E.; Nicholson, L.K.; Separovic, F.; Wallace, B.A. // Nature Structural Biology;Jul99, Vol. 6 Issue 7, p610 

    Suggests that the intertwined double-stranded gramicidin A structures do not correlate well with the experimental data obtained from a lipid environment, which uniquely defines the single-stranded structure. Structures of gramicidin A in membrane mimetic environments; Reason that the...

  • Gramicidin channel controversy ? reply. Burkhart, Brian M.; Duax, William L. // Nature Structural Biology;Jul99, Vol. 6 Issue 7, p611 

    Explains the rationale for the suggestion that the active conformation of gramicidin A may be the double-stranded double-helical dimer structure instead of the more traditional head-to-head single-stranded dimer model. Reason for the criticism against the crystal structure determinations;...

  • Gravity dependency of the gramicidin A channel conductivity: A model for gravity perception on the cellular level. Schatz, A.; Linke-Hommes, A.; Neubert, J. // European Biophysics Journal;1996, Vol. 25 Issue 1, p37 

    Theoretical investigations involving the membrane-solution interface have revealed that the density of the solution varies appreciably within interfacial layers adjacent to charged membrane surfaces. The hypothesis that gravity interacts with this configuration and modifies transport rates...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics